Expression of glyoxylate cycle genes in cucumber roots responds to sugar supply and can be activated by shading or defoliation of the shoot

Ismanizan Ismail, Luigi De Bellis, Amedeo Alpi, Steven M. Smith

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

When cucumber roots are excised and incubated without a carbon source, isocitrate lyase (ICL) and malate synthase (MS) mRNAs increase significantly in amount. However, if sucrose is added to the excised roots, the mRNAs do not accumulate. Hairy roots obtained by transformation with Agrobacterium rhizogenes show the same response. Transgenic hairy roots containing the Icl and Ms gene promoters fused to the GUS reporter gene, have very low GUS activity which increases dramatically when roots are incubated in the absence of sugar, indicating regulation at the transcriptional level. Staining of sugar-deprived roots shows that GUS activity is concentrated mainly in root tips and lateral root primordia, where demand for carbohydrate is greatest. In order to determine if Icl and Ms genes are expressed in roots of whole plants under conditions which may occur in nature, cucumber plants were subjected to reduced light intensity or defoliation. In both cases increases were observed in ICL and MS mRNAs. These treatments also reduced root sugar content, consistent with the hypothesis that sugar supply could control expression of Icl and Ms genes in roots of whole plants.

Original languageEnglish
Pages (from-to)633-640
Number of pages8
JournalPlant Molecular Biology
Volume35
Issue number5
DOIs
Publication statusPublished - Nov 1997
Externally publishedYes

Fingerprint

glyoxylate cycle
Cucumis sativus
Malate Synthase
Isocitrate Lyase
defoliation
Sugars
cucumbers
shade
Plant Roots
Genes
sugars
Messenger RNA
shoots
Agrobacterium
genes
Meristem
Reporter Genes
malate synthase
isocitrate lyase
Sucrose

Keywords

  • Agrobacterium rhizogenes
  • Carbohydrate regulation
  • Cucumber (Cucumis sativus L.)
  • Gene expression
  • Glyoxylate cycle
  • Hairy roots

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Expression of glyoxylate cycle genes in cucumber roots responds to sugar supply and can be activated by shading or defoliation of the shoot. / Ismail, Ismanizan; De Bellis, Luigi; Alpi, Amedeo; Smith, Steven M.

In: Plant Molecular Biology, Vol. 35, No. 5, 11.1997, p. 633-640.

Research output: Contribution to journalArticle

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