Expression and purification of a recombinant scFv towards the exotoxin of the pathogen, Burkholderia pseudomallei

Kue Peng Lim, HongBin Li, Sheila Nathan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A single chain variable fragment (scFv) specific towards B. pseudomallei exotoxin had previously been generated from an existing hybridoma cell line (6E6AF83B) and cloned into the phage display vector pComb3H. In this study, the scFv was subcloned into the pComb3X vector to facilitate the detection and purification of expressed antibodies. Detection was facilitated by the presence of a hemagglutinin (HA) tag, and purification was facilitated by the presence of a histidine tag. The culture was grown at 30°C until log phase was achieved and then induced with 1 mM IPTG in the absence of any additional carbon source. Induction was continued at 30°C for five h. The scFv was discerned by dual processes-direct enzyme-linked immunosorbent assays (ELISA), and Western blotting. When compared to E. coli strains ER2537 and HB2151, scFv expression was observed to be highest in the E. coli strain Top10F′. The expressed scFv protein was purified via nickel-mediated affinity chromatography and results indicated that two proteins a 52 kDa protein, and a 30 kDa protein were co-purified. These antibodies, when blotted against immobilized exotoxin, exhibited significant specificity towards the exotoxin, compared to other B. pseudomallei antigens. Thus, these antibodies should serve as suitable reagents for future affinity purification of the exotoxin.

Original languageEnglish
Pages (from-to)126-132
Number of pages7
JournalJournal of Microbiology
Volume42
Issue number2
Publication statusPublished - Jun 2004

Fingerprint

Burkholderia pseudomallei
Exotoxins
Antibodies
Proteins
Escherichia coli
Isopropyl Thiogalactoside
Single-Chain Antibodies
Hybridomas
Hemagglutinins
Nickel
Affinity Chromatography
Histidine
Bacteriophages
Carbon
Western Blotting
Enzyme-Linked Immunosorbent Assay
Antigens
Cell Line

Keywords

  • Affinity chromatography
  • Exotoxin
  • Monoclonal antibody
  • Phage display
  • scFv

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Expression and purification of a recombinant scFv towards the exotoxin of the pathogen, Burkholderia pseudomallei. / Lim, Kue Peng; Li, HongBin; Nathan, Sheila.

In: Journal of Microbiology, Vol. 42, No. 2, 06.2004, p. 126-132.

Research output: Contribution to journalArticle

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