Expression and mutational analysis of GATA3 in Malaysian breast carcinomas

Pau Ni Ivyna Bong, Zubaidah Zakaria, Rohaizak Muhammad, Norlia Abdullah, Naqiyah Ibrahim, Nor Aina Emran, Sharifah Noor Akmal Syed Hussain

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The GATA3 gene is a potential tumour marker and putative tumour suppressor gene in breast cancer. Its expression is associated with better prognosis and disease free survival in breast cancer patients. We aimed to evaluate GATA3 transcriptome expression and mutation in breast carcinomas and correlate its expression with oestrogen receptor (ER), progesterone receptor (PR), lymph node (LN) status, tumour grade and c-erbB-2 expression. Twenty-two breast infi ltrating ductal carcinomas and paired normal tissues were used in Branch DNA assay to detect GATA3 mRNA expression. Normalized data for GATA3 mRNA expression were grouped according to the ER, PR and LN status, tumour grade and c-erbB-2 expression of the tumours. Statistical signifi cance was tested using t-test and ANOVA at 95% confi dence interval level. Mutational analysis of GATA3 was performed by direct sequencing of the coding regions of GATA3 mRNA. Our findings showed that GATA3 gene were over-expressed and under-expressed by >2 fold change in 12 and 4 tested samples, respectively. Eighty per cent of ER positive breast carcinomas were GATA3 positive. There was a statistically signifi cant correlation between GATA3 expression and ER at 95% confi dence interval level between the study groups. On the contrary, GATA3 expression was not statistically signifi cant with PR, LN, tumour grade and c-erbB-2 expression in our study. In addition, we observed that there was no mutation in mRNA coding region in 16 breast carcinomas that showed GATA3 differential gene expression. Our preliminary results suggested that GATA3 is linked to the ER. This scenario suggests that GATA3 may play a crucial role in oestrogen receptor positive breast cancer patients. Whether GATA3 expression is involved in regulating tumour cell growth in oestrogen responsive breast cancer is a key question that remains to be answered.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalMalaysian Journal of Pathology
Volume32
Issue number2
Publication statusPublished - Dec 2010

Fingerprint

Breast Neoplasms
Progesterone Receptors
Messenger RNA
Lymph Nodes
Neoplasms
Estrogen Receptors
Ductal Carcinoma
Mutation
Tumor Biomarkers
Tumor Suppressor Genes
Transcriptome
Genes
Disease-Free Survival
Analysis of Variance
Estrogens
Breast
Gene Expression
DNA
Growth

Keywords

  • Breast cancer
  • Differential expression
  • Mutation
  • Oestrogen receptor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Ivyna Bong, P. N., Zakaria, Z., Muhammad, R., Abdullah, N., Ibrahim, N., Emran, N. A., & Syed Hussain, S. N. A. (2010). Expression and mutational analysis of GATA3 in Malaysian breast carcinomas. Malaysian Journal of Pathology, 32(2), 117-122.

Expression and mutational analysis of GATA3 in Malaysian breast carcinomas. / Ivyna Bong, Pau Ni; Zakaria, Zubaidah; Muhammad, Rohaizak; Abdullah, Norlia; Ibrahim, Naqiyah; Emran, Nor Aina; Syed Hussain, Sharifah Noor Akmal.

In: Malaysian Journal of Pathology, Vol. 32, No. 2, 12.2010, p. 117-122.

Research output: Contribution to journalArticle

Ivyna Bong, PN, Zakaria, Z, Muhammad, R, Abdullah, N, Ibrahim, N, Emran, NA & Syed Hussain, SNA 2010, 'Expression and mutational analysis of GATA3 in Malaysian breast carcinomas', Malaysian Journal of Pathology, vol. 32, no. 2, pp. 117-122.
Ivyna Bong, Pau Ni ; Zakaria, Zubaidah ; Muhammad, Rohaizak ; Abdullah, Norlia ; Ibrahim, Naqiyah ; Emran, Nor Aina ; Syed Hussain, Sharifah Noor Akmal. / Expression and mutational analysis of GATA3 in Malaysian breast carcinomas. In: Malaysian Journal of Pathology. 2010 ; Vol. 32, No. 2. pp. 117-122.
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abstract = "The GATA3 gene is a potential tumour marker and putative tumour suppressor gene in breast cancer. Its expression is associated with better prognosis and disease free survival in breast cancer patients. We aimed to evaluate GATA3 transcriptome expression and mutation in breast carcinomas and correlate its expression with oestrogen receptor (ER), progesterone receptor (PR), lymph node (LN) status, tumour grade and c-erbB-2 expression. Twenty-two breast infi ltrating ductal carcinomas and paired normal tissues were used in Branch DNA assay to detect GATA3 mRNA expression. Normalized data for GATA3 mRNA expression were grouped according to the ER, PR and LN status, tumour grade and c-erbB-2 expression of the tumours. Statistical signifi cance was tested using t-test and ANOVA at 95{\%} confi dence interval level. Mutational analysis of GATA3 was performed by direct sequencing of the coding regions of GATA3 mRNA. Our findings showed that GATA3 gene were over-expressed and under-expressed by >2 fold change in 12 and 4 tested samples, respectively. Eighty per cent of ER positive breast carcinomas were GATA3 positive. There was a statistically signifi cant correlation between GATA3 expression and ER at 95{\%} confi dence interval level between the study groups. On the contrary, GATA3 expression was not statistically signifi cant with PR, LN, tumour grade and c-erbB-2 expression in our study. In addition, we observed that there was no mutation in mRNA coding region in 16 breast carcinomas that showed GATA3 differential gene expression. Our preliminary results suggested that GATA3 is linked to the ER. This scenario suggests that GATA3 may play a crucial role in oestrogen receptor positive breast cancer patients. Whether GATA3 expression is involved in regulating tumour cell growth in oestrogen responsive breast cancer is a key question that remains to be answered.",
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