Expression and characterization of a cutinase (AnCUT2) from Aspergillus Niger

Khadijah Ahmed Al-Tammar, Othman Omar, Abdul Munir Abd. Murad, Farah Diba Abu Bakar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cutin hydrolase (EC 3.1.1.74), an extracellular polyesterase found in pollens, bacteria and fungi, is an efficient catalyst that exhibits hydrolytic activity on a variety of water-soluble esters, synthetic fibers, plastics and triglycerides. Thus, cutinase can be used in various applications such as ester synthesis, bio-scouring, food and detergent industries. Ancut2 is one of five genes encoding cutinases present in the Aspergillus Niger ATCC 10574 genome. The cDNA of Ancut2 comprising of an open reading frame of 816 bp encoding a protein of 271 amino acid residues, was isolated and expressed in Pichia pastoris. The partially purified recombinant cutinase exhibited a molecular mass of approximately 40 kDa. The enzyme showed highest activity at 40°C with a preference for acidic pH (5.0-6.0). AnCUT2 showed hydrolytic activity towards various p-nitrophenyl esters with preference towards shorter chain esters such as p-nitrophenyl butyrate (C4). Scanning Electron Microscopy demonstrated that AnCUT2 was capable of modifying surfaces of synthetic polycaprolactone and polyethylene terephthalate plastics. The properties of this enzyme suggest that it may be applied in synthetic fiber modification and fruit processing industries.

Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalOpen Life Sciences
Volume11
Issue number1
DOIs
Publication statusPublished - 1 Mar 2016

Fingerprint

cutinase
Aspergillus niger
Aspergillus
Esters
esters
Synthetic fibers
synthetic fibers
Plastics
plastics
Polyethylene Terephthalates
Gene encoding
Pichia
Food Industry
Molecular mass
industry
Hydrolases
Enzymes
Fruits
Pollen
Fungi

Keywords

  • heterologous expression
  • p-nitrophenyl esters
  • Pichia pastoris
  • polycaprolactone
  • polyethylene terephthalate
  • scanning electron microscopy
  • Serine esterase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Expression and characterization of a cutinase (AnCUT2) from Aspergillus Niger. / Ahmed Al-Tammar, Khadijah; Omar, Othman; Abd. Murad, Abdul Munir; Abu Bakar, Farah Diba.

In: Open Life Sciences, Vol. 11, No. 1, 01.03.2016, p. 29-38.

Research output: Contribution to journalArticle

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