Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing

Shiplu Roy Chowdhury, Lim Shaun Jing, Muhammad Noor Haziq Bin Zolkafli, Nur' Aqilah Binti Mohd Abu Zarin, Wan Alyani Binti Wan Abdullah, Nurul Aidah Binti Md Mothar, Manira Maarof, Abdullah Nur Atiqah Haizum

Research output: Contribution to journalArticle

Abstract

Skin ageing is associated with a decrease in collagen and delayed wound healing. The previous study has shown that supplementation of growth factors can enhance the production of extracellular matrix (ECM) and efficiency of wound healing. This study aimed to produce a Dermal Fibroblast Conditioned Medium (DFCM) and evaluate its potential on in vitro skin wound healing and anti-ageing properties. DFCM were obtained by culturing confluent human dermal fibroblasts (n=3) in keratinocyte-specific medium (DFCM-KM), fibroblast-specific medium (DFCM-FM) and fibroblast-specific medium with growth supplements (DFCM-GM). Protein concentration was determined by Bicinchoninic Acid (BCA) assay. To evaluate the wound healing and anti-ageing properties, human dermal fibroblasts were supplemented with three different DFCM. Fibroblasts cultured with F12:DMEM+10%FBS was used as a control. The growth rate of fibroblasts was evaluated by culturing cells until day 4. Migration rate was evaluated at 12 h after seeding. In vitro healing rate was evaluated via scratch assay. Anti-ageing potential of DFCM was evaluated via RT 2 Profiler PCR Array. Concentration of total protein was found to be significantly higher in DFCM-GM (2914.79±150.67mg/mL) compared to DFCM-KM and DFCM-FM. Out of the tested 12 anti-ageing genes, fibroblasts supplemented with DFCM-GM demonstrated a significantly higher expression of WRN compared to other conditions. In conclusion, DFCM-GM contains more proteins and found superior in modulating wound healing and anti-ageing properties.

Original languageEnglish
Pages (from-to)637-644
Number of pages8
JournalSains Malaysiana
Volume48
Issue number3
DOIs
Publication statusPublished - 1 Mar 2019

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Conditioned Culture Medium
Wound Healing
Fibroblasts
Skin
Skin Aging
Proteins
Growth
Keratinocytes

Keywords

  • Anti-ageing
  • Conditioned medium
  • Dermal fibroblast conditioned medium
  • Fibroblasts
  • Wound healing

ASJC Scopus subject areas

  • General

Cite this

Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing. / Chowdhury, Shiplu Roy; Jing, Lim Shaun; Zolkafli, Muhammad Noor Haziq Bin; Zarin, Nur' Aqilah Binti Mohd Abu; Abdullah, Wan Alyani Binti Wan; Md Mothar, Nurul Aidah Binti; Maarof, Manira; Nur Atiqah Haizum, Abdullah.

In: Sains Malaysiana, Vol. 48, No. 3, 01.03.2019, p. 637-644.

Research output: Contribution to journalArticle

Chowdhury, Shiplu Roy ; Jing, Lim Shaun ; Zolkafli, Muhammad Noor Haziq Bin ; Zarin, Nur' Aqilah Binti Mohd Abu ; Abdullah, Wan Alyani Binti Wan ; Md Mothar, Nurul Aidah Binti ; Maarof, Manira ; Nur Atiqah Haizum, Abdullah. / Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing. In: Sains Malaysiana. 2019 ; Vol. 48, No. 3. pp. 637-644.
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AU - Zolkafli, Muhammad Noor Haziq Bin

AU - Zarin, Nur' Aqilah Binti Mohd Abu

AU - Abdullah, Wan Alyani Binti Wan

AU - Md Mothar, Nurul Aidah Binti

AU - Maarof, Manira

AU - Nur Atiqah Haizum, Abdullah

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N2 - Skin ageing is associated with a decrease in collagen and delayed wound healing. The previous study has shown that supplementation of growth factors can enhance the production of extracellular matrix (ECM) and efficiency of wound healing. This study aimed to produce a Dermal Fibroblast Conditioned Medium (DFCM) and evaluate its potential on in vitro skin wound healing and anti-ageing properties. DFCM were obtained by culturing confluent human dermal fibroblasts (n=3) in keratinocyte-specific medium (DFCM-KM), fibroblast-specific medium (DFCM-FM) and fibroblast-specific medium with growth supplements (DFCM-GM). Protein concentration was determined by Bicinchoninic Acid (BCA) assay. To evaluate the wound healing and anti-ageing properties, human dermal fibroblasts were supplemented with three different DFCM. Fibroblasts cultured with F12:DMEM+10%FBS was used as a control. The growth rate of fibroblasts was evaluated by culturing cells until day 4. Migration rate was evaluated at 12 h after seeding. In vitro healing rate was evaluated via scratch assay. Anti-ageing potential of DFCM was evaluated via RT 2 Profiler PCR Array. Concentration of total protein was found to be significantly higher in DFCM-GM (2914.79±150.67mg/mL) compared to DFCM-KM and DFCM-FM. Out of the tested 12 anti-ageing genes, fibroblasts supplemented with DFCM-GM demonstrated a significantly higher expression of WRN compared to other conditions. In conclusion, DFCM-GM contains more proteins and found superior in modulating wound healing and anti-ageing properties.

AB - Skin ageing is associated with a decrease in collagen and delayed wound healing. The previous study has shown that supplementation of growth factors can enhance the production of extracellular matrix (ECM) and efficiency of wound healing. This study aimed to produce a Dermal Fibroblast Conditioned Medium (DFCM) and evaluate its potential on in vitro skin wound healing and anti-ageing properties. DFCM were obtained by culturing confluent human dermal fibroblasts (n=3) in keratinocyte-specific medium (DFCM-KM), fibroblast-specific medium (DFCM-FM) and fibroblast-specific medium with growth supplements (DFCM-GM). Protein concentration was determined by Bicinchoninic Acid (BCA) assay. To evaluate the wound healing and anti-ageing properties, human dermal fibroblasts were supplemented with three different DFCM. Fibroblasts cultured with F12:DMEM+10%FBS was used as a control. The growth rate of fibroblasts was evaluated by culturing cells until day 4. Migration rate was evaluated at 12 h after seeding. In vitro healing rate was evaluated via scratch assay. Anti-ageing potential of DFCM was evaluated via RT 2 Profiler PCR Array. Concentration of total protein was found to be significantly higher in DFCM-GM (2914.79±150.67mg/mL) compared to DFCM-KM and DFCM-FM. Out of the tested 12 anti-ageing genes, fibroblasts supplemented with DFCM-GM demonstrated a significantly higher expression of WRN compared to other conditions. In conclusion, DFCM-GM contains more proteins and found superior in modulating wound healing and anti-ageing properties.

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