Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens

Abang Masli Dayang Izawati, Mat Yunus Abdul Masani, Ismanizan Ismail, Ghulam Kadir Ahmad Parveez

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

DOGR1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOGR1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l1 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOGR1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOGR1 gene and 2-DOG for regenerating transgenic oil palm.

Original languageEnglish
Article number727
JournalFrontiers in Plant Science
Volume6
Issue numberSeptember
DOIs
Publication statusPublished - 23 Sep 2015

Fingerprint

2-deoxyglucose
Elaeis guineensis
Agrobacterium radiobacter
callus
phosphates
plantlets
genes
biosafety
Southern blotting
transgenes
transgenic plants
genetically modified organisms
genetic markers
genome
soil

Keywords

  • 2-deoxyglucose
  • Agrobacterium-mediated transformation
  • DOG<sup>R</sup> 1 gene
  • Oil palm embryogenic calli

ASJC Scopus subject areas

  • Plant Science

Cite this

Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens. / Izawati, Abang Masli Dayang; Masani, Mat Yunus Abdul; Ismail, Ismanizan; Parveez, Ghulam Kadir Ahmad.

In: Frontiers in Plant Science, Vol. 6, No. September, 727, 23.09.2015.

Research output: Contribution to journalArticle

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