Abstract
DOGR1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOGR1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l−1 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOGR1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOGR1 gene and 2-DOG for regenerating transgenic oil palm.
| Original language | English |
|---|---|
| Article number | 727 |
| Journal | Frontiers in Plant Science |
| Volume | 6 |
| Issue number | September |
| DOIs | |
| Publication status | Published - 23 Sep 2015 |
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Keywords
- 2-deoxyglucose
- Agrobacterium-mediated transformation
- DOG<sup>R</sup> 1 gene
- Oil palm embryogenic calli
ASJC Scopus subject areas
- Plant Science
Cite this
Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens. / Izawati, Abang Masli Dayang; Masani, Mat Yunus Abdul; Ismail, Ismanizan; Parveez, Ghulam Kadir Ahmad.
In: Frontiers in Plant Science, Vol. 6, No. September, 727, 23.09.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens
AU - Izawati, Abang Masli Dayang
AU - Masani, Mat Yunus Abdul
AU - Ismail, Ismanizan
AU - Parveez, Ghulam Kadir Ahmad
PY - 2015/9/23
Y1 - 2015/9/23
N2 - DOGR1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOGR1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l−1 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOGR1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOGR1 gene and 2-DOG for regenerating transgenic oil palm.
AB - DOGR1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOGR1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l−1 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOGR1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOGR1 gene and 2-DOG for regenerating transgenic oil palm.
KW - 2-deoxyglucose
KW - Agrobacterium-mediated transformation
KW - DOG<sup>R</sup> 1 gene
KW - Oil palm embryogenic calli
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UR - http://www.scopus.com/inward/citedby.url?scp=84942848133&partnerID=8YFLogxK
U2 - 10.3389/fpls.2015.00727
DO - 10.3389/fpls.2015.00727
M3 - Article
VL - 6
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
IS - September
M1 - 727
ER -