Evaluation of variable parameters in neutrophil function test using chemiluminescence assay.

R. M. Noah, M. R. Jais, Lokman Mohd. Noh

Research output: Contribution to journalArticle

Abstract

Variable parameters in chemiluminescence assay, one of the methods used to assess the functional capacity of neutrophils, were evaluated for suitable adaptation locally. The use of pooled normal human serum as compared to single normal human serum in opsonizing particles for phagocytosis was found to exhibit lower chemiluminescence activity (reduction range of 30%-50%). A similar degree of depression was observed when the particles were opsonized using normal human serum in comparison to that using autologous serum. Different intensity of chemiluminescence was also noted when the opsonized particle used was the Oxford strain of Staphylococcus aureus (NCTC 6571) in contrast to a strain of Staphylococcus aureus isolated from a patient. The results obtained warrant clinicians to deliver appropriate samples as best they can when the chemiluminescence assay is requested.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalThe Malaysian journal of pathology
Volume16
Issue number2
Publication statusPublished - Dec 1994

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Luminescence
Neutrophils
Serum
Staphylococcus aureus
Phagocytosis

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Evaluation of variable parameters in neutrophil function test using chemiluminescence assay. / Noah, R. M.; Jais, M. R.; Mohd. Noh, Lokman.

In: The Malaysian journal of pathology, Vol. 16, No. 2, 12.1994, p. 157-160.

Research output: Contribution to journalArticle

Noah, R. M. ; Jais, M. R. ; Mohd. Noh, Lokman. / Evaluation of variable parameters in neutrophil function test using chemiluminescence assay. In: The Malaysian journal of pathology. 1994 ; Vol. 16, No. 2. pp. 157-160.
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