Evaluation of the Ex vivo antimalarial activity of organotin (IV) ethylphenyldithiocarbamate on erythrocytes infected with Plasmodium berghei Nk 65

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Malaria is the most destructive and dangerous parasitic disease. The commonness of this disease is getting worse mainly due to the increasing resistance ofPlasmodium falciparum against antimalarial drugs. Therefore, the search for new antimalarial drug is urgently needed. This study was carried out to evaluate the effects of dibutyltin (IV) ethylphenyldithiocarbamate (DBEP), diphenyltin(IV) ethylphenyldithiocarbamate (DPEP) and triphenyltin (IV) ethylphenyldithiocarbamate (TPEP) compounds as antimalarial agents. These compounds were evaluated against erythrocytes infected with Plasmodium berghei NK65 via ex vivo. Organotin (IV) ethylphenyldithiocarbamate, [RnSn(C9H10NS2)4.n] with R = C4H9 and CJT5 for n = 2; R = CJT5 for n = 3 is chemically synthesised for its potential activities. pLDH assay was employed for determination of the concentration that inhibited 50% of the Plasmodium's activity (IC50) after 24 h treatment at concentration range of 10-0.0000001 mg mL-1. Plasmodium berghei NK65 was cultured in vitro to determine the different morphology of trophozoite and schizont. Only DPEP and TPEP compounds have antimalarial activity towards P. bergheiNK65 at IC50 0.094±0.011 and 0.892±0.088 mg mL1, respectively. The IC50 of DPEP and TPEP were lowest at 30% parasitemia with IC50 0.001±0.00009 and 0.0009±0.0001 mg mL1, respectively. In vitro culture showed that TPEP was effective towards/3, berghei ~NK65 in trophozoite and schizont morphology with IC500.0001±0.00005 and 0.00009±0.00003 jag mL1respectively. In conclusion, DPEP and TPEP have antimalarial effect on erythrocytes infected with P. berghei NK65 and have potential as antimalarial and schizonticidal agents.

Original languageEnglish
Pages (from-to)836-842
Number of pages7
JournalPakistan Journal of Biological Sciences
Volume17
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Plasmodium berghei
antimalarials
erythrocytes
inhibitory concentration 50
schizonts
trophozoites
parasitemia
Plasmodium
parasitoses
in vitro culture
malaria
assays

Keywords

  • Antimalaria
  • Organotin (IV) ethylphenyldithiocarbamate
  • Plasmodium berghei
  • pLDH assay
  • Triphenyltin (IV) ethylphenyldithiocarbamate

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{3521d3add48440afb546eb15911e2f40,
title = "Evaluation of the Ex vivo antimalarial activity of organotin (IV) ethylphenyldithiocarbamate on erythrocytes infected with Plasmodium berghei Nk 65",
abstract = "Malaria is the most destructive and dangerous parasitic disease. The commonness of this disease is getting worse mainly due to the increasing resistance ofPlasmodium falciparum against antimalarial drugs. Therefore, the search for new antimalarial drug is urgently needed. This study was carried out to evaluate the effects of dibutyltin (IV) ethylphenyldithiocarbamate (DBEP), diphenyltin(IV) ethylphenyldithiocarbamate (DPEP) and triphenyltin (IV) ethylphenyldithiocarbamate (TPEP) compounds as antimalarial agents. These compounds were evaluated against erythrocytes infected with Plasmodium berghei NK65 via ex vivo. Organotin (IV) ethylphenyldithiocarbamate, [RnSn(C9H10NS2)4.n] with R = C4H9 and CJT5 for n = 2; R = CJT5 for n = 3 is chemically synthesised for its potential activities. pLDH assay was employed for determination of the concentration that inhibited 50{\%} of the Plasmodium's activity (IC50) after 24 h treatment at concentration range of 10-0.0000001 mg mL-1. Plasmodium berghei NK65 was cultured in vitro to determine the different morphology of trophozoite and schizont. Only DPEP and TPEP compounds have antimalarial activity towards P. bergheiNK65 at IC50 0.094±0.011 and 0.892±0.088 mg mL1, respectively. The IC50 of DPEP and TPEP were lowest at 30{\%} parasitemia with IC50 0.001±0.00009 and 0.0009±0.0001 mg mL1, respectively. In vitro culture showed that TPEP was effective towards/3, berghei ~NK65 in trophozoite and schizont morphology with IC500.0001±0.00005 and 0.00009±0.00003 jag mL1respectively. In conclusion, DPEP and TPEP have antimalarial effect on erythrocytes infected with P. berghei NK65 and have potential as antimalarial and schizonticidal agents.",
keywords = "Antimalaria, Organotin (IV) ethylphenyldithiocarbamate, Plasmodium berghei, pLDH assay, Triphenyltin (IV) ethylphenyldithiocarbamate",
author = "Normah Awang and Hafizah Jumat and Shak, {Shafariatul Akmar} and {Nurul Farahana}, Kamaludin",
year = "2014",
doi = "10.3923/pjbs.2014.836.842",
language = "English",
volume = "17",
pages = "836--842",
journal = "Pakistan Journal of Biological Sciences",
issn = "1028-8880",
publisher = "Asian Network for Scientific Information",
number = "6",

}

TY - JOUR

T1 - Evaluation of the Ex vivo antimalarial activity of organotin (IV) ethylphenyldithiocarbamate on erythrocytes infected with Plasmodium berghei Nk 65

AU - Awang, Normah

AU - Jumat, Hafizah

AU - Shak, Shafariatul Akmar

AU - Nurul Farahana, Kamaludin

PY - 2014

Y1 - 2014

N2 - Malaria is the most destructive and dangerous parasitic disease. The commonness of this disease is getting worse mainly due to the increasing resistance ofPlasmodium falciparum against antimalarial drugs. Therefore, the search for new antimalarial drug is urgently needed. This study was carried out to evaluate the effects of dibutyltin (IV) ethylphenyldithiocarbamate (DBEP), diphenyltin(IV) ethylphenyldithiocarbamate (DPEP) and triphenyltin (IV) ethylphenyldithiocarbamate (TPEP) compounds as antimalarial agents. These compounds were evaluated against erythrocytes infected with Plasmodium berghei NK65 via ex vivo. Organotin (IV) ethylphenyldithiocarbamate, [RnSn(C9H10NS2)4.n] with R = C4H9 and CJT5 for n = 2; R = CJT5 for n = 3 is chemically synthesised for its potential activities. pLDH assay was employed for determination of the concentration that inhibited 50% of the Plasmodium's activity (IC50) after 24 h treatment at concentration range of 10-0.0000001 mg mL-1. Plasmodium berghei NK65 was cultured in vitro to determine the different morphology of trophozoite and schizont. Only DPEP and TPEP compounds have antimalarial activity towards P. bergheiNK65 at IC50 0.094±0.011 and 0.892±0.088 mg mL1, respectively. The IC50 of DPEP and TPEP were lowest at 30% parasitemia with IC50 0.001±0.00009 and 0.0009±0.0001 mg mL1, respectively. In vitro culture showed that TPEP was effective towards/3, berghei ~NK65 in trophozoite and schizont morphology with IC500.0001±0.00005 and 0.00009±0.00003 jag mL1respectively. In conclusion, DPEP and TPEP have antimalarial effect on erythrocytes infected with P. berghei NK65 and have potential as antimalarial and schizonticidal agents.

AB - Malaria is the most destructive and dangerous parasitic disease. The commonness of this disease is getting worse mainly due to the increasing resistance ofPlasmodium falciparum against antimalarial drugs. Therefore, the search for new antimalarial drug is urgently needed. This study was carried out to evaluate the effects of dibutyltin (IV) ethylphenyldithiocarbamate (DBEP), diphenyltin(IV) ethylphenyldithiocarbamate (DPEP) and triphenyltin (IV) ethylphenyldithiocarbamate (TPEP) compounds as antimalarial agents. These compounds were evaluated against erythrocytes infected with Plasmodium berghei NK65 via ex vivo. Organotin (IV) ethylphenyldithiocarbamate, [RnSn(C9H10NS2)4.n] with R = C4H9 and CJT5 for n = 2; R = CJT5 for n = 3 is chemically synthesised for its potential activities. pLDH assay was employed for determination of the concentration that inhibited 50% of the Plasmodium's activity (IC50) after 24 h treatment at concentration range of 10-0.0000001 mg mL-1. Plasmodium berghei NK65 was cultured in vitro to determine the different morphology of trophozoite and schizont. Only DPEP and TPEP compounds have antimalarial activity towards P. bergheiNK65 at IC50 0.094±0.011 and 0.892±0.088 mg mL1, respectively. The IC50 of DPEP and TPEP were lowest at 30% parasitemia with IC50 0.001±0.00009 and 0.0009±0.0001 mg mL1, respectively. In vitro culture showed that TPEP was effective towards/3, berghei ~NK65 in trophozoite and schizont morphology with IC500.0001±0.00005 and 0.00009±0.00003 jag mL1respectively. In conclusion, DPEP and TPEP have antimalarial effect on erythrocytes infected with P. berghei NK65 and have potential as antimalarial and schizonticidal agents.

KW - Antimalaria

KW - Organotin (IV) ethylphenyldithiocarbamate

KW - Plasmodium berghei

KW - pLDH assay

KW - Triphenyltin (IV) ethylphenyldithiocarbamate

UR - http://www.scopus.com/inward/record.url?scp=84893414030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893414030&partnerID=8YFLogxK

U2 - 10.3923/pjbs.2014.836.842

DO - 10.3923/pjbs.2014.836.842

M3 - Article

C2 - 26035957

AN - SCOPUS:84893414030

VL - 17

SP - 836

EP - 842

JO - Pakistan Journal of Biological Sciences

JF - Pakistan Journal of Biological Sciences

SN - 1028-8880

IS - 6

ER -