Evaluation of polymerase chain reaction (PCR) method and hybrid capture II (HCII) assay for the detection of human papillomavirus in cervical scrapings

R. Saini, T. H. Shen, N. H. Othman, Jacinta Santhanam, N. Othman, Thean Hock Tang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In order to investigate the reliability of detecting HPV DNA in cervical smears, we compared the performance of nested MY/GP PCR and FDA approved-Hybrid Capture II (HCII) using clinical cervical scrapings from 40 patients. It was found that PCR was more sensitive (81.8%) in comparison to HCII (36.4%) in detecting HPV although specificity of HCII was much higher (96.6%) than PCR (58.6%). The Negative Predictive Value (NPV) of both the techniques were quite similar but Positive Predictive Value (PPV) of HCII was much higher (80.0%) compared to PCR (42.9%). While the HCII method showed good specificity for HPV detection, its lack of sensitivity as compared to PCR may be a drawback for diagnostic use.

Original languageEnglish
Pages (from-to)206-209
Number of pages4
JournalMedical Journal of Malaysia
Volume62
Issue number3
Publication statusPublished - Aug 2007

Fingerprint

Polymerase Chain Reaction
Vaginal Smears
DNA

Keywords

  • HPV
  • Hybrid capture II
  • PCR

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Evaluation of polymerase chain reaction (PCR) method and hybrid capture II (HCII) assay for the detection of human papillomavirus in cervical scrapings. / Saini, R.; Shen, T. H.; Othman, N. H.; Santhanam, Jacinta; Othman, N.; Tang, Thean Hock.

In: Medical Journal of Malaysia, Vol. 62, No. 3, 08.2007, p. 206-209.

Research output: Contribution to journalArticle

@article{2d424bc26abd42e99eea460b0da415d7,
title = "Evaluation of polymerase chain reaction (PCR) method and hybrid capture II (HCII) assay for the detection of human papillomavirus in cervical scrapings",
abstract = "In order to investigate the reliability of detecting HPV DNA in cervical smears, we compared the performance of nested MY/GP PCR and FDA approved-Hybrid Capture II (HCII) using clinical cervical scrapings from 40 patients. It was found that PCR was more sensitive (81.8{\%}) in comparison to HCII (36.4{\%}) in detecting HPV although specificity of HCII was much higher (96.6{\%}) than PCR (58.6{\%}). The Negative Predictive Value (NPV) of both the techniques were quite similar but Positive Predictive Value (PPV) of HCII was much higher (80.0{\%}) compared to PCR (42.9{\%}). While the HCII method showed good specificity for HPV detection, its lack of sensitivity as compared to PCR may be a drawback for diagnostic use.",
keywords = "HPV, Hybrid capture II, PCR",
author = "R. Saini and Shen, {T. H.} and Othman, {N. H.} and Jacinta Santhanam and N. Othman and Tang, {Thean Hock}",
year = "2007",
month = "8",
language = "English",
volume = "62",
pages = "206--209",
journal = "Medical Journal of Malaysia",
issn = "0300-5283",
publisher = "Malaysian Medical Association",
number = "3",

}

TY - JOUR

T1 - Evaluation of polymerase chain reaction (PCR) method and hybrid capture II (HCII) assay for the detection of human papillomavirus in cervical scrapings

AU - Saini, R.

AU - Shen, T. H.

AU - Othman, N. H.

AU - Santhanam, Jacinta

AU - Othman, N.

AU - Tang, Thean Hock

PY - 2007/8

Y1 - 2007/8

N2 - In order to investigate the reliability of detecting HPV DNA in cervical smears, we compared the performance of nested MY/GP PCR and FDA approved-Hybrid Capture II (HCII) using clinical cervical scrapings from 40 patients. It was found that PCR was more sensitive (81.8%) in comparison to HCII (36.4%) in detecting HPV although specificity of HCII was much higher (96.6%) than PCR (58.6%). The Negative Predictive Value (NPV) of both the techniques were quite similar but Positive Predictive Value (PPV) of HCII was much higher (80.0%) compared to PCR (42.9%). While the HCII method showed good specificity for HPV detection, its lack of sensitivity as compared to PCR may be a drawback for diagnostic use.

AB - In order to investigate the reliability of detecting HPV DNA in cervical smears, we compared the performance of nested MY/GP PCR and FDA approved-Hybrid Capture II (HCII) using clinical cervical scrapings from 40 patients. It was found that PCR was more sensitive (81.8%) in comparison to HCII (36.4%) in detecting HPV although specificity of HCII was much higher (96.6%) than PCR (58.6%). The Negative Predictive Value (NPV) of both the techniques were quite similar but Positive Predictive Value (PPV) of HCII was much higher (80.0%) compared to PCR (42.9%). While the HCII method showed good specificity for HPV detection, its lack of sensitivity as compared to PCR may be a drawback for diagnostic use.

KW - HPV

KW - Hybrid capture II

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=34548612545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548612545&partnerID=8YFLogxK

M3 - Article

C2 - 18246908

AN - SCOPUS:34548612545

VL - 62

SP - 206

EP - 209

JO - Medical Journal of Malaysia

JF - Medical Journal of Malaysia

SN - 0300-5283

IS - 3

ER -