Evaluation of mutagenic profile of shrimp paste extracts by using ames test

Nur Hafiza Mohammad, Nurlaily Ezzaty Mohamad Yusoff, Siti Shahara Zulfakar, Razinah Sharif @ Mohd Sharif

Research output: Contribution to journalArticle

Abstract

Shrimp paste (belacan) has been used as a condiment in many Malaysian cooking. This study aimed to determine the mutagenicity of aqueous extract of shrimp paste from three different places in Pulau Pinang, Malaysia (Balik Pulau, Juru and Pulau Aman) by using Ames test. The test was performed using Salmonella typhimurium strain, TA98 and TA100, in presence and absence of S9 metabolic activation system. Five concentrations of the samples tested were 50, 25, 12.5, 6.25 and 3.125 mg/ml. Macronutrients and mineral content of shrimp paste tested were complied with the Malaysian food act 1983 and Recommended Nutrient Intake (RNI) 2005. Ames test result of shrimp paste from all three places showed number of revertant colonies did not exceed the value of double-fold of negative control either in presence and absence of S9. No significant difference (p>0.05) was found for each concentration tested on the number of revertant colonies between TA 98 and TA 100.

Original languageEnglish
Pages (from-to)170-175
Number of pages6
JournalPakistan Journal of Nutrition
Volume15
Issue number2
DOIs
Publication statusPublished - 2016

Fingerprint

Ames test
Ointments
shrimp
extracts
Condiments
Amantadine
Food
condiments
mutagenicity
Malaysia
Cooking
Salmonella typhimurium
Salmonella Typhimurium
nutrient intake
mineral content
Minerals
cooking
water
testing
sampling

Keywords

  • Mineral content
  • Mutagenicity
  • Shrimp paste

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics
  • Public Health, Environmental and Occupational Health

Cite this

Evaluation of mutagenic profile of shrimp paste extracts by using ames test. / Mohammad, Nur Hafiza; Yusoff, Nurlaily Ezzaty Mohamad; Zulfakar, Siti Shahara; Sharif @ Mohd Sharif, Razinah.

In: Pakistan Journal of Nutrition, Vol. 15, No. 2, 2016, p. 170-175.

Research output: Contribution to journalArticle

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