Evaluation of Morphological Changes of Staphylococcus aureus and Escherichia coli Induced with the Antimicrobial Peptide AN5-1

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The mechanisms of action of AN5-1 against Gram-negative and Gram-positive bacteria were investigated by evaluations of the intracellular content leakage and by microscopic observations of the treated cells. Escherichia coli and Staphylococcus aureus were used for this investigation. Measurements of DNA, RNA, proteins, and β-galactosidase were taken, and the results showed a significant increase in the cultivation media after treatment with AN5-1 compared with the untreated cells. The morphological changes of treated cells were shown using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The observations showed that AN5-1 acts against E. coli and against S. aureus in similar ways, by targeting the cell wall, causing disruptions; at a high concentration (80 AU/ml), these disruptions led to cell lysis. The 3D AFM imaging system showed that at a low concentration of 20 AU/ml, the effect of AN5-1 is restricted to pore formation only. Moreover, a separation between the cell wall and the cytoplasm was observed when Gram-negative bacteria were treated with a low concentration (20 AU/ml) of AN5-1.

Original languageEnglish
Pages (from-to)1868-1878
Number of pages11
JournalApplied Biochemistry and Biotechnology
Volume175
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Escherichia coli
Peptides
Staphylococcus aureus
Cells
Atomic Force Microscopy
Atomic force microscopy
Bacteria
Cell Wall
Galactosidases
RNA
Imaging systems
Gram-Positive Bacteria
Gram-Negative Bacteria
DNA
Transmission Electron Microscopy
Transmission electron microscopy
Proteins
Cytoplasm
gastrofenzin

Keywords

  • Antimicrobial peptide AN5-1
  • Atomic force microscope
  • Mode action
  • Paenibacillus alvei AN5
  • Transmission electron microscope

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

@article{a84576632300463a900fbb19805eb71c,
title = "Evaluation of Morphological Changes of Staphylococcus aureus and Escherichia coli Induced with the Antimicrobial Peptide AN5-1",
abstract = "The mechanisms of action of AN5-1 against Gram-negative and Gram-positive bacteria were investigated by evaluations of the intracellular content leakage and by microscopic observations of the treated cells. Escherichia coli and Staphylococcus aureus were used for this investigation. Measurements of DNA, RNA, proteins, and β-galactosidase were taken, and the results showed a significant increase in the cultivation media after treatment with AN5-1 compared with the untreated cells. The morphological changes of treated cells were shown using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The observations showed that AN5-1 acts against E. coli and against S. aureus in similar ways, by targeting the cell wall, causing disruptions; at a high concentration (80 AU/ml), these disruptions led to cell lysis. The 3D AFM imaging system showed that at a low concentration of 20 AU/ml, the effect of AN5-1 is restricted to pore formation only. Moreover, a separation between the cell wall and the cytoplasm was observed when Gram-negative bacteria were treated with a low concentration (20 AU/ml) of AN5-1.",
keywords = "Antimicrobial peptide AN5-1, Atomic force microscope, Mode action, Paenibacillus alvei AN5, Transmission electron microscope",
author = "Bassam Alkotaini and Nurina Anuar and Kadhum, {Abdul Amir H.}",
year = "2014",
doi = "10.1007/s12010-014-1410-4",
language = "English",
volume = "175",
pages = "1868--1878",
journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",
issn = "0273-2289",
publisher = "Humana Press",
number = "4",

}

TY - JOUR

T1 - Evaluation of Morphological Changes of Staphylococcus aureus and Escherichia coli Induced with the Antimicrobial Peptide AN5-1

AU - Alkotaini, Bassam

AU - Anuar, Nurina

AU - Kadhum, Abdul Amir H.

PY - 2014

Y1 - 2014

N2 - The mechanisms of action of AN5-1 against Gram-negative and Gram-positive bacteria were investigated by evaluations of the intracellular content leakage and by microscopic observations of the treated cells. Escherichia coli and Staphylococcus aureus were used for this investigation. Measurements of DNA, RNA, proteins, and β-galactosidase were taken, and the results showed a significant increase in the cultivation media after treatment with AN5-1 compared with the untreated cells. The morphological changes of treated cells were shown using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The observations showed that AN5-1 acts against E. coli and against S. aureus in similar ways, by targeting the cell wall, causing disruptions; at a high concentration (80 AU/ml), these disruptions led to cell lysis. The 3D AFM imaging system showed that at a low concentration of 20 AU/ml, the effect of AN5-1 is restricted to pore formation only. Moreover, a separation between the cell wall and the cytoplasm was observed when Gram-negative bacteria were treated with a low concentration (20 AU/ml) of AN5-1.

AB - The mechanisms of action of AN5-1 against Gram-negative and Gram-positive bacteria were investigated by evaluations of the intracellular content leakage and by microscopic observations of the treated cells. Escherichia coli and Staphylococcus aureus were used for this investigation. Measurements of DNA, RNA, proteins, and β-galactosidase were taken, and the results showed a significant increase in the cultivation media after treatment with AN5-1 compared with the untreated cells. The morphological changes of treated cells were shown using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The observations showed that AN5-1 acts against E. coli and against S. aureus in similar ways, by targeting the cell wall, causing disruptions; at a high concentration (80 AU/ml), these disruptions led to cell lysis. The 3D AFM imaging system showed that at a low concentration of 20 AU/ml, the effect of AN5-1 is restricted to pore formation only. Moreover, a separation between the cell wall and the cytoplasm was observed when Gram-negative bacteria were treated with a low concentration (20 AU/ml) of AN5-1.

KW - Antimicrobial peptide AN5-1

KW - Atomic force microscope

KW - Mode action

KW - Paenibacillus alvei AN5

KW - Transmission electron microscope

UR - http://www.scopus.com/inward/record.url?scp=84922439121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922439121&partnerID=8YFLogxK

U2 - 10.1007/s12010-014-1410-4

DO - 10.1007/s12010-014-1410-4

M3 - Article

VL - 175

SP - 1868

EP - 1878

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

SN - 0273-2289

IS - 4

ER -