Abstract
The mechanisms of action of AN5-1 against Gram-negative and Gram-positive bacteria were investigated by evaluations of the intracellular content leakage and by microscopic observations of the treated cells. Escherichia coli and Staphylococcus aureus were used for this investigation. Measurements of DNA, RNA, proteins, and β-galactosidase were taken, and the results showed a significant increase in the cultivation media after treatment with AN5-1 compared with the untreated cells. The morphological changes of treated cells were shown using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The observations showed that AN5-1 acts against E. coli and against S. aureus in similar ways, by targeting the cell wall, causing disruptions; at a high concentration (80 AU/ml), these disruptions led to cell lysis. The 3D AFM imaging system showed that at a low concentration of 20 AU/ml, the effect of AN5-1 is restricted to pore formation only. Moreover, a separation between the cell wall and the cytoplasm was observed when Gram-negative bacteria were treated with a low concentration (20 AU/ml) of AN5-1.
| Original language | English |
|---|---|
| Pages (from-to) | 1868-1878 |
| Number of pages | 11 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 175 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Antimicrobial peptide AN5-1
- Atomic force microscope
- Mode action
- Paenibacillus alvei AN5
- Transmission electron microscope
ASJC Scopus subject areas
- Biochemistry
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Molecular Biology
Cite this
Evaluation of Morphological Changes of Staphylococcus aureus and Escherichia coli Induced with the Antimicrobial Peptide AN5-1. / Alkotaini, Bassam; Anuar, Nurina; Kadhum, Abdul Amir H.
In: Applied Biochemistry and Biotechnology, Vol. 175, No. 4, 2014, p. 1868-1878.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of Morphological Changes of Staphylococcus aureus and Escherichia coli Induced with the Antimicrobial Peptide AN5-1
AU - Alkotaini, Bassam
AU - Anuar, Nurina
AU - Kadhum, Abdul Amir H.
PY - 2014
Y1 - 2014
N2 - The mechanisms of action of AN5-1 against Gram-negative and Gram-positive bacteria were investigated by evaluations of the intracellular content leakage and by microscopic observations of the treated cells. Escherichia coli and Staphylococcus aureus were used for this investigation. Measurements of DNA, RNA, proteins, and β-galactosidase were taken, and the results showed a significant increase in the cultivation media after treatment with AN5-1 compared with the untreated cells. The morphological changes of treated cells were shown using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The observations showed that AN5-1 acts against E. coli and against S. aureus in similar ways, by targeting the cell wall, causing disruptions; at a high concentration (80 AU/ml), these disruptions led to cell lysis. The 3D AFM imaging system showed that at a low concentration of 20 AU/ml, the effect of AN5-1 is restricted to pore formation only. Moreover, a separation between the cell wall and the cytoplasm was observed when Gram-negative bacteria were treated with a low concentration (20 AU/ml) of AN5-1.
AB - The mechanisms of action of AN5-1 against Gram-negative and Gram-positive bacteria were investigated by evaluations of the intracellular content leakage and by microscopic observations of the treated cells. Escherichia coli and Staphylococcus aureus were used for this investigation. Measurements of DNA, RNA, proteins, and β-galactosidase were taken, and the results showed a significant increase in the cultivation media after treatment with AN5-1 compared with the untreated cells. The morphological changes of treated cells were shown using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The observations showed that AN5-1 acts against E. coli and against S. aureus in similar ways, by targeting the cell wall, causing disruptions; at a high concentration (80 AU/ml), these disruptions led to cell lysis. The 3D AFM imaging system showed that at a low concentration of 20 AU/ml, the effect of AN5-1 is restricted to pore formation only. Moreover, a separation between the cell wall and the cytoplasm was observed when Gram-negative bacteria were treated with a low concentration (20 AU/ml) of AN5-1.
KW - Antimicrobial peptide AN5-1
KW - Atomic force microscope
KW - Mode action
KW - Paenibacillus alvei AN5
KW - Transmission electron microscope
UR - http://www.scopus.com/inward/record.url?scp=84922439121&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922439121&partnerID=8YFLogxK
U2 - 10.1007/s12010-014-1410-4
DO - 10.1007/s12010-014-1410-4
M3 - Article
C2 - 25427593
AN - SCOPUS:84922439121
VL - 175
SP - 1868
EP - 1878
JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
SN - 0273-2289
IS - 4
ER -