Evaluation of interleukin-17 and interleukin-23 in pterygium

Immunohistochemistry study

Kiew Ing Tiong, Aida Zairani Mohd Zahidin, S. Kala Sumugam, Joseph Uchang, Hazlita Mohd Isa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: to compare the interleukin-17 (il-17) and interleukin-23 (il-23) positive cell counts between pterygium and normal conjunctiva. Design: a case-control study. Methods: this study received ethical approval (nMrr research id 23957) and informed consent was obtained from all participants. it involved 20 participants with 20 samples of pterygium and 20 samples of normal conjunctiva that were obtained from the same eye of each participant. all the participants underwent history taking, slit lamp examination, and pterygium excision surgery. Both samples underwent im-munohistochemistry procedure. Pretreatment procedure was conducted using heat-induced epitope retrieval with Pt link, subsequently followed by EnVision FLEX staining procedure and incubation with anti?IL-17 antibody and anti?IL-23 antibody. Slides were examined in high-power fields (400x) for both samples in 3 different fields. total positive stained cell counts in all 3 fields with il-17 and il-23 between pterygium and normal conjunctiva were analyzed by using Wilcoxon signed rank test. Results: il-17 positive cell counts for normal conjunctiva showed mean 196.10 ± 80.487 but for pterygium was 331.10 ± 108.416. as for il-23, the mean for positive cell counts for normal conjunctiva was 62.10 ± 33.462 and il-23 positive cell counts for pterygium showed mean 102.95 ± 41.378. Both il-17 and il-23 were significantly increased in pterygium compared with normal conjunctiva (P < 0.001). Conclusions: Both il-17 and il-23 were found to be significantly higher in the pterygium group than in the normal conjunctiva group with P < 0.001 by Wilcoxon signed rank test.

Original languageEnglish
Pages (from-to)403-406
Number of pages4
JournalAsia-Pacific Journal of Ophthalmology
Volume6
Issue number5
DOIs
Publication statusPublished - 1 Oct 2017
Externally publishedYes

Fingerprint

Interleukin-23
Pterygium
Interleukin-17
Conjunctiva
Immunohistochemistry
Cell Count
Nonparametric Statistics
Informed Consent
Case-Control Studies
Epitopes
Anti-Idiotypic Antibodies
Hot Temperature
Staining and Labeling
Antibodies

Keywords

  • Interleukin-17
  • Interleukin-23
  • Pterygium

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Evaluation of interleukin-17 and interleukin-23 in pterygium : Immunohistochemistry study. / Tiong, Kiew Ing; Mohd Zahidin, Aida Zairani; Kala Sumugam, S.; Uchang, Joseph; Mohd Isa, Hazlita.

In: Asia-Pacific Journal of Ophthalmology, Vol. 6, No. 5, 01.10.2017, p. 403-406.

Research output: Contribution to journalArticle

Tiong, KI, Mohd Zahidin, AZ, Kala Sumugam, S, Uchang, J & Mohd Isa, H 2017, 'Evaluation of interleukin-17 and interleukin-23 in pterygium: Immunohistochemistry study', Asia-Pacific Journal of Ophthalmology, vol. 6, no. 5, pp. 403-406. https://doi.org/10.22608/aPo.2017134
Tiong, Kiew Ing ; Mohd Zahidin, Aida Zairani ; Kala Sumugam, S. ; Uchang, Joseph ; Mohd Isa, Hazlita. / Evaluation of interleukin-17 and interleukin-23 in pterygium : Immunohistochemistry study. In: Asia-Pacific Journal of Ophthalmology. 2017 ; Vol. 6, No. 5. pp. 403-406.
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T1 - Evaluation of interleukin-17 and interleukin-23 in pterygium

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AU - Tiong, Kiew Ing

AU - Mohd Zahidin, Aida Zairani

AU - Kala Sumugam, S.

AU - Uchang, Joseph

AU - Mohd Isa, Hazlita

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AB - Purpose: to compare the interleukin-17 (il-17) and interleukin-23 (il-23) positive cell counts between pterygium and normal conjunctiva. Design: a case-control study. Methods: this study received ethical approval (nMrr research id 23957) and informed consent was obtained from all participants. it involved 20 participants with 20 samples of pterygium and 20 samples of normal conjunctiva that were obtained from the same eye of each participant. all the participants underwent history taking, slit lamp examination, and pterygium excision surgery. Both samples underwent im-munohistochemistry procedure. Pretreatment procedure was conducted using heat-induced epitope retrieval with Pt link, subsequently followed by EnVision FLEX staining procedure and incubation with anti?IL-17 antibody and anti?IL-23 antibody. Slides were examined in high-power fields (400x) for both samples in 3 different fields. total positive stained cell counts in all 3 fields with il-17 and il-23 between pterygium and normal conjunctiva were analyzed by using Wilcoxon signed rank test. Results: il-17 positive cell counts for normal conjunctiva showed mean 196.10 ± 80.487 but for pterygium was 331.10 ± 108.416. as for il-23, the mean for positive cell counts for normal conjunctiva was 62.10 ± 33.462 and il-23 positive cell counts for pterygium showed mean 102.95 ± 41.378. Both il-17 and il-23 were significantly increased in pterygium compared with normal conjunctiva (P < 0.001). Conclusions: Both il-17 and il-23 were found to be significantly higher in the pterygium group than in the normal conjunctiva group with P < 0.001 by Wilcoxon signed rank test.

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KW - Interleukin-23

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