Evaluation of Escherichia coli Enumeration Methods in Poultry Dishes

Translated title of the contribution: Evaluation of escherichia coli enumeration methods in poultry dishes

Ratna Dewi Abdul Rahman, Norrakiah Abdullah Sani, Abdul Aziz Jemain

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The purpose of this study was to select the best from eleven methods for Escherichia coli enumeration in poultry dishes. The methods were pour, spread and drop platig, Petrifilm™, three types of direct plating and four types of most probable number (MPN) methods. Method comparison was carried out based on ISO 16140 procedure. Each type of E. coli strains (ATCC 25922, IMR 1/3 107B and IMR E243) were inoculated into five types of poultry dishes to obtain 10 5 cfu/g bacterial concentration. Heat treatment was done at 55°C for 4-6 min to mimic the environment as these foods were served right after cooking. The Ordinary Least-Squares (OLS) Regression statistical analysis was carried out to compare eleven methods for E. coli enumeration. The significance (p<0.05) of gradient value (m) for all OLS Graphs mean that all methods were similar in term of accuracy, correlation and relative accuracy. Thus, practical approaches were also considered in order to select the best from eleven methods for E. coli enumeration in poultry dishes. Pour plating, drop plating and Petrifilm™ methods were more practical than the other eight methods evaluated.

Original languageUndefined/Unknown
Pages (from-to)325-331
Number of pages7
JournalSains Malaysiana
Volume41
Issue number3
Publication statusPublished - Mar 2012

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poultry
Escherichia coli
methodology
least squares
cooking
statistical analysis
heat treatment

Keywords

  • Escherichia coli
  • ISO 16140 method
  • OLS regression
  • Poultry dishes

ASJC Scopus subject areas

  • General

Cite this

Evaluation of Escherichia coli Enumeration Methods in Poultry Dishes. / Rahman, Ratna Dewi Abdul; Abdullah Sani, Norrakiah; Jemain, Abdul Aziz.

In: Sains Malaysiana, Vol. 41, No. 3, 03.2012, p. 325-331.

Research output: Contribution to journalArticle

Rahman, Ratna Dewi Abdul ; Abdullah Sani, Norrakiah ; Jemain, Abdul Aziz. / Evaluation of Escherichia coli Enumeration Methods in Poultry Dishes. In: Sains Malaysiana. 2012 ; Vol. 41, No. 3. pp. 325-331.
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