Evaluation of DNA and RNA extraction methods

C. S. Edwin Shiaw, M. S. Shiran, Yoke Kqueen Cheah, Geok Chin Tan, A. R. Sabariah

Research output: Contribution to journalArticle

Abstract

This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex-100 extraction method (Method B), heat-induced retrieval in alkaline solution extraction method (Methods C and D) and one commercial FFPE DNA Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2 extraction protocols were evaluated including the enzymatic extraction method (Method 1), and Chelex-100 RNA extraction method (Method 2). Results show that the modified enzymatic extraction method (Method A) is an efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalMedical Journal of Malaysia
Volume65
Issue number2
Publication statusPublished - Jun 2010
Externally publishedYes

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Keywords

  • Crosslinking
  • DNA extraction
  • FFPE
  • RNA extraction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Edwin Shiaw, C. S., Shiran, M. S., Cheah, Y. K., Tan, G. C., & Sabariah, A. R. (2010). Evaluation of DNA and RNA extraction methods. Medical Journal of Malaysia, 65(2), 133-137.

Evaluation of DNA and RNA extraction methods. / Edwin Shiaw, C. S.; Shiran, M. S.; Cheah, Yoke Kqueen; Tan, Geok Chin; Sabariah, A. R.

In: Medical Journal of Malaysia, Vol. 65, No. 2, 06.2010, p. 133-137.

Research output: Contribution to journalArticle

Edwin Shiaw, CS, Shiran, MS, Cheah, YK, Tan, GC & Sabariah, AR 2010, 'Evaluation of DNA and RNA extraction methods', Medical Journal of Malaysia, vol. 65, no. 2, pp. 133-137.
Edwin Shiaw CS, Shiran MS, Cheah YK, Tan GC, Sabariah AR. Evaluation of DNA and RNA extraction methods. Medical Journal of Malaysia. 2010 Jun;65(2):133-137.
Edwin Shiaw, C. S. ; Shiran, M. S. ; Cheah, Yoke Kqueen ; Tan, Geok Chin ; Sabariah, A. R. / Evaluation of DNA and RNA extraction methods. In: Medical Journal of Malaysia. 2010 ; Vol. 65, No. 2. pp. 133-137.
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