Evaluation of bitter melon (Momordica charantia) extract administration in the antioxidant and free radical scavenging activities of plasma and liver in male rat

K. Amira, Aminah Abdullah, A. Zuhair

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of this study was to determine the antioxidant activities of dietary bitter melon fruit supplemented in male rat model. In this study, three common tests for measuring antioxidant activity of the bitter melon were evaluated using 2, 2-diphenyl-l-picrylhydrazyl assay (DPPH assay), total phenolic acid assay (TPC assay) and Ferric reducing ability of plasma assa. (FRAP assay). Results showed that the extracting solvent significantly (P<0.05) altered the antioxidant property estimations of bitter melon fruit. Pure solvents were inefficient extraction media for antioxidant. Enhanced extraction yields were obtained from solvent containing higher water concentrations and 50% acetone is a recommended solvent for extracting antioxidants compounds from bitter melon fruit. High levels of antioxidant activities were detected in plasma and liver in treatment group compared to the control group, indicating that the fruit may serve as an excellent dietary source of natural antioxidants. The correlation between total phenolics and the antioxidant activities of bitter melon showed significant positive correlations (r2). These findings support the notion that the bitter melon's a good source of bioactive compounds. From the study it was observed that there was a significantly increased (p ≤ 0.05) in plasma total antioxidant capacity in rat after the consumption of bitter melon.

Original languageEnglish
Pages (from-to)319-323
Number of pages5
JournalInternational Food Research Journal
Volume20
Issue number1
Publication statusPublished - 2013

Fingerprint

Momordica charantia
Free Radicals
Antioxidants
antioxidants
liver
Liver
rats
extracts
antioxidant activity
Fruit
fruits
assays
phenolic acids
biphenyl
acetone
Acetone
animal models

Keywords

  • Bitter melon
  • DPPH radical scavenging activity
  • Extraction solvent
  • Phenolic content
  • Plasma antioxidant capability

ASJC Scopus subject areas

  • Food Science

Cite this

@article{713eb374a19345b29fbe742185f44e2d,
title = "Evaluation of bitter melon (Momordica charantia) extract administration in the antioxidant and free radical scavenging activities of plasma and liver in male rat",
abstract = "The aim of this study was to determine the antioxidant activities of dietary bitter melon fruit supplemented in male rat model. In this study, three common tests for measuring antioxidant activity of the bitter melon were evaluated using 2, 2-diphenyl-l-picrylhydrazyl assay (DPPH assay), total phenolic acid assay (TPC assay) and Ferric reducing ability of plasma assa. (FRAP assay). Results showed that the extracting solvent significantly (P<0.05) altered the antioxidant property estimations of bitter melon fruit. Pure solvents were inefficient extraction media for antioxidant. Enhanced extraction yields were obtained from solvent containing higher water concentrations and 50{\%} acetone is a recommended solvent for extracting antioxidants compounds from bitter melon fruit. High levels of antioxidant activities were detected in plasma and liver in treatment group compared to the control group, indicating that the fruit may serve as an excellent dietary source of natural antioxidants. The correlation between total phenolics and the antioxidant activities of bitter melon showed significant positive correlations (r2). These findings support the notion that the bitter melon's a good source of bioactive compounds. From the study it was observed that there was a significantly increased (p ≤ 0.05) in plasma total antioxidant capacity in rat after the consumption of bitter melon.",
keywords = "Bitter melon, DPPH radical scavenging activity, Extraction solvent, Phenolic content, Plasma antioxidant capability",
author = "K. Amira and Aminah Abdullah and A. Zuhair",
year = "2013",
language = "English",
volume = "20",
pages = "319--323",
journal = "International Food Research Journal",
issn = "1985-4668",
publisher = "Universiti Putra Malaysia",
number = "1",

}

TY - JOUR

T1 - Evaluation of bitter melon (Momordica charantia) extract administration in the antioxidant and free radical scavenging activities of plasma and liver in male rat

AU - Amira, K.

AU - Abdullah, Aminah

AU - Zuhair, A.

PY - 2013

Y1 - 2013

N2 - The aim of this study was to determine the antioxidant activities of dietary bitter melon fruit supplemented in male rat model. In this study, three common tests for measuring antioxidant activity of the bitter melon were evaluated using 2, 2-diphenyl-l-picrylhydrazyl assay (DPPH assay), total phenolic acid assay (TPC assay) and Ferric reducing ability of plasma assa. (FRAP assay). Results showed that the extracting solvent significantly (P<0.05) altered the antioxidant property estimations of bitter melon fruit. Pure solvents were inefficient extraction media for antioxidant. Enhanced extraction yields were obtained from solvent containing higher water concentrations and 50% acetone is a recommended solvent for extracting antioxidants compounds from bitter melon fruit. High levels of antioxidant activities were detected in plasma and liver in treatment group compared to the control group, indicating that the fruit may serve as an excellent dietary source of natural antioxidants. The correlation between total phenolics and the antioxidant activities of bitter melon showed significant positive correlations (r2). These findings support the notion that the bitter melon's a good source of bioactive compounds. From the study it was observed that there was a significantly increased (p ≤ 0.05) in plasma total antioxidant capacity in rat after the consumption of bitter melon.

AB - The aim of this study was to determine the antioxidant activities of dietary bitter melon fruit supplemented in male rat model. In this study, three common tests for measuring antioxidant activity of the bitter melon were evaluated using 2, 2-diphenyl-l-picrylhydrazyl assay (DPPH assay), total phenolic acid assay (TPC assay) and Ferric reducing ability of plasma assa. (FRAP assay). Results showed that the extracting solvent significantly (P<0.05) altered the antioxidant property estimations of bitter melon fruit. Pure solvents were inefficient extraction media for antioxidant. Enhanced extraction yields were obtained from solvent containing higher water concentrations and 50% acetone is a recommended solvent for extracting antioxidants compounds from bitter melon fruit. High levels of antioxidant activities were detected in plasma and liver in treatment group compared to the control group, indicating that the fruit may serve as an excellent dietary source of natural antioxidants. The correlation between total phenolics and the antioxidant activities of bitter melon showed significant positive correlations (r2). These findings support the notion that the bitter melon's a good source of bioactive compounds. From the study it was observed that there was a significantly increased (p ≤ 0.05) in plasma total antioxidant capacity in rat after the consumption of bitter melon.

KW - Bitter melon

KW - DPPH radical scavenging activity

KW - Extraction solvent

KW - Phenolic content

KW - Plasma antioxidant capability

UR - http://www.scopus.com/inward/record.url?scp=84871482429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871482429&partnerID=8YFLogxK

M3 - Article

VL - 20

SP - 319

EP - 323

JO - International Food Research Journal

JF - International Food Research Journal

SN - 1985-4668

IS - 1

ER -