Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (rubiaceae family) leaves

Suhanya Parthasarathy, Juzaili Bin Azizi, Surash Ramanathan, Sabariah Ismail, Sreenivasan Sasidharan, Mohd Ikram Mohd, Sharif Mahsufi Mansor

    Research output: Contribution to journalArticle

    55 Citations (Scopus)

    Abstract

    Studies on the antioxidant and antimicrobial activities of Mitragyna speciosa leaf extracts are lacking. In this study the antioxidant properties of water, methanolic and alkaloid M. speciosa leaf extracts were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. The amount of total phenolics and flavanoid contents were also estimated. The DPPH IC 50 values of the aqueous, alkaloid and methanolic extracts were 213.4, 104.81 and 37.08 μg/mL, respectively. The total phenolic content of the aqueous, alkaloid and methanolic extracts were 66.0 mg, 88.4, 105.6 mg GAE/g, respectively, while the total flavanoid were 28.2, 20.0 and 91.1 mg CAE/g respectively. The antioxidant activities were correlated with the total phenolic content. This result suggests that the relatively high antioxidant activity of the methanolic extract compared to aqueous and alkaloid extract could be possibly be due to its high phenolic content. The aqueous, alkaloid and methanolic extracts were screened for antimicrobial activity. The extracts showed antimicrobial activity against Salmonella typhi and Bacillus subtilis. The minimum inhibitory concentrations (MICs) of extracts determined by the broth dilution method ranged from 3.12 to 6.25 mg/mL. The alkaloid extract was found to be most effective against all of the tested organisms.

    Original languageEnglish
    Pages (from-to)3964-3974
    Number of pages11
    JournalMolecules
    Volume14
    Issue number10
    DOIs
    Publication statusPublished - Oct 2009

    Fingerprint

    Mitragyna
    Family Leave
    Rubiaceae
    alkaloids
    antioxidants
    Alkaloids
    leaves
    Antioxidants
    evaluation
    broths
    salmonella
    Salmonella typhi
    Salmonella
    Bacillus
    Computer aided engineering
    Scavenging
    scavenging
    Microbial Sensitivity Tests
    Bacilli
    Bacillus subtilis

    Keywords

    • Antibacterial
    • Antioxidant
    • Mitragyna speciosa

    ASJC Scopus subject areas

    • Organic Chemistry

    Cite this

    Parthasarathy, S., Azizi, J. B., Ramanathan, S., Ismail, S., Sasidharan, S., Mohd, M. I., & Mansor, S. M. (2009). Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (rubiaceae family) leaves. Molecules, 14(10), 3964-3974. https://doi.org/10.3390/molecules14103964

    Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (rubiaceae family) leaves. / Parthasarathy, Suhanya; Azizi, Juzaili Bin; Ramanathan, Surash; Ismail, Sabariah; Sasidharan, Sreenivasan; Mohd, Mohd Ikram; Mansor, Sharif Mahsufi.

    In: Molecules, Vol. 14, No. 10, 10.2009, p. 3964-3974.

    Research output: Contribution to journalArticle

    Parthasarathy, S, Azizi, JB, Ramanathan, S, Ismail, S, Sasidharan, S, Mohd, MI & Mansor, SM 2009, 'Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (rubiaceae family) leaves', Molecules, vol. 14, no. 10, pp. 3964-3974. https://doi.org/10.3390/molecules14103964
    Parthasarathy, Suhanya ; Azizi, Juzaili Bin ; Ramanathan, Surash ; Ismail, Sabariah ; Sasidharan, Sreenivasan ; Mohd, Mohd Ikram ; Mansor, Sharif Mahsufi. / Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (rubiaceae family) leaves. In: Molecules. 2009 ; Vol. 14, No. 10. pp. 3964-3974.
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