Evaluation and Bulked Segregant Analysis of Major Yield QTL qtl12.1 Introgressed into Indigenous Elite Line for Low Water Availability under Water Stress

N. Manikanda Boopathi, Gat Swapnashri, P. Kavitha, S. Sathish, R. Nithya, R Wickneswari V Ratnam, Arvind Kumar

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Near isogenic lines carrying large-effect QTL (qtl12.1), which has a consistent influence on grain yield under upland drought stress conditions in a wide range of environments, were evaluated under water stress in the fields. The line which gave higher yield under drought was crossed with a local elite line, PMK3, and forwarded to F2:3 generation. Significant variation was found among the F2:3 lines for agronomic traits under water stress in the fields. Low to high broad sense heritability (H) for investigated traits was also found. Water stress indicators such as leaf rolling and leaf drying were negatively correlated with plant height, biomass and grain yield under stress. Bulked segregant analysis (BSA) was performed with the markers in the vicinity of qtl12.1, and RM27933 was found to be segregated perfectly well in individual components of drought resistant and drought susceptible bulks which were bulked based on yield under water stress among F2:3 lines. Hence, this simple and breeder friendly marker, RM27933, may be useful as a potentially valuable candidate marker for the transfer of the QTL qtl12.1 in the regional breeding program. Bioinformatic analysis of the DNA sequence of the qtl12.1 region was also done to identify and analyze positional candidate genes associated with this QTL and to ascertain the putative molecular basis of qtl12.1.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalRice Science
Volume20
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Droughts
Dehydration
water stress
Water
drought
water
grain yield
Genetic Association Studies
leaf rolling
Computational Biology
DNA Sequence Analysis
Biomass
Breeding
isogenic lines
bioinformatics
agronomic traits
heritability
highlands
drying
nucleotide sequences

Keywords

  • Bulked segregant analysis
  • Marker-assisted selection
  • Quantitative trait locus
  • Rice
  • Water stress

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Biotechnology
  • Plant Science

Cite this

Evaluation and Bulked Segregant Analysis of Major Yield QTL qtl12.1 Introgressed into Indigenous Elite Line for Low Water Availability under Water Stress. / Boopathi, N. Manikanda; Swapnashri, Gat; Kavitha, P.; Sathish, S.; Nithya, R.; V Ratnam, R Wickneswari; Kumar, Arvind.

In: Rice Science, Vol. 20, No. 1, 01.2013, p. 25-30.

Research output: Contribution to journalArticle

Boopathi, N. Manikanda ; Swapnashri, Gat ; Kavitha, P. ; Sathish, S. ; Nithya, R. ; V Ratnam, R Wickneswari ; Kumar, Arvind. / Evaluation and Bulked Segregant Analysis of Major Yield QTL qtl12.1 Introgressed into Indigenous Elite Line for Low Water Availability under Water Stress. In: Rice Science. 2013 ; Vol. 20, No. 1. pp. 25-30.
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