Evaluating the efficacy of Trichoderma spp and Bacillus substilis as biocontrol agents against Magnaporthe grisea in rice

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Rice blast causes yield losses to rice farmers worldwide. Although this problem is currently being addressed through the use of resistant rice varieties, fungicide and rotation farming, these methods alone do not form a durable, long lasting solution in mitigating disease. Here we obtained Trichoderma isolates from soil and tested their efficacy as biocontrol agents against Magnaporthe grisea. Twenty-two Trichoderma isolates were identified and used in dual culture assays to determine antagonistic ability before isolates showing promise were administered to rice plants grown in greenhouse conditions where the efficacy of these isolates was tested individually or as dual inoculums with Bacillus substilis UKM1. The results showed that the dual inoculation of biocontrol agents caused significant (p ≤0.05) inhibition of M. grisea as compared to a single agent. In addition, we observed the inhibitory effect of Trichoderma T2 under SEM where the hypha of the biocontrol agent coiled and possibly enzymatically degraded the pathogen. Trichoderma T2 in combination with B. substilis UKM1 gave the highest reduction in pre-post damping off, disease infection and disease severity caused by M. grisea in greenhouse conditions. From the greenhouse experimentations, the biocontrol agents were more efficient in inhibiting rice blast when applied prior to transplantation as compared to when applications were made at seed setting stage.

Original languageEnglish
Pages (from-to)1324-1335
Number of pages12
JournalAustralian Journal of Crop Science
Volume8
Issue number9
Publication statusPublished - 2014

Fingerprint

Magnaporthe grisea
Trichoderma
biological control agents
rice
blast disease
greenhouses
damping off
greenhouse experimentation
disease severity
hyphae
fungicides
farming systems
farmers
pathogens
assays
seeds
infection
soil
methodology

Keywords

  • Bacillus substilis UKM1
  • Biocontrol
  • Dual plate culture
  • Hyphal coiling
  • Magnaporthe grisea
  • Trichoderma spp

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{5f79d8ed74a24f5a85c96009324b0735,
title = "Evaluating the efficacy of Trichoderma spp and Bacillus substilis as biocontrol agents against Magnaporthe grisea in rice",
abstract = "Rice blast causes yield losses to rice farmers worldwide. Although this problem is currently being addressed through the use of resistant rice varieties, fungicide and rotation farming, these methods alone do not form a durable, long lasting solution in mitigating disease. Here we obtained Trichoderma isolates from soil and tested their efficacy as biocontrol agents against Magnaporthe grisea. Twenty-two Trichoderma isolates were identified and used in dual culture assays to determine antagonistic ability before isolates showing promise were administered to rice plants grown in greenhouse conditions where the efficacy of these isolates was tested individually or as dual inoculums with Bacillus substilis UKM1. The results showed that the dual inoculation of biocontrol agents caused significant (p ≤0.05) inhibition of M. grisea as compared to a single agent. In addition, we observed the inhibitory effect of Trichoderma T2 under SEM where the hypha of the biocontrol agent coiled and possibly enzymatically degraded the pathogen. Trichoderma T2 in combination with B. substilis UKM1 gave the highest reduction in pre-post damping off, disease infection and disease severity caused by M. grisea in greenhouse conditions. From the greenhouse experimentations, the biocontrol agents were more efficient in inhibiting rice blast when applied prior to transplantation as compared to when applications were made at seed setting stage.",
keywords = "Bacillus substilis UKM1, Biocontrol, Dual plate culture, Hyphal coiling, Magnaporthe grisea, Trichoderma spp",
author = "Hamdia Ali and {K. Nadarajah}, Kalaivani",
year = "2014",
language = "English",
volume = "8",
pages = "1324--1335",
journal = "Australian Journal of Crop Science",
issn = "1835-2693",
publisher = "Southern Cross Publishing and Printing Pty Ltd",
number = "9",

}

TY - JOUR

T1 - Evaluating the efficacy of Trichoderma spp and Bacillus substilis as biocontrol agents against Magnaporthe grisea in rice

AU - Ali, Hamdia

AU - K. Nadarajah, Kalaivani

PY - 2014

Y1 - 2014

N2 - Rice blast causes yield losses to rice farmers worldwide. Although this problem is currently being addressed through the use of resistant rice varieties, fungicide and rotation farming, these methods alone do not form a durable, long lasting solution in mitigating disease. Here we obtained Trichoderma isolates from soil and tested their efficacy as biocontrol agents against Magnaporthe grisea. Twenty-two Trichoderma isolates were identified and used in dual culture assays to determine antagonistic ability before isolates showing promise were administered to rice plants grown in greenhouse conditions where the efficacy of these isolates was tested individually or as dual inoculums with Bacillus substilis UKM1. The results showed that the dual inoculation of biocontrol agents caused significant (p ≤0.05) inhibition of M. grisea as compared to a single agent. In addition, we observed the inhibitory effect of Trichoderma T2 under SEM where the hypha of the biocontrol agent coiled and possibly enzymatically degraded the pathogen. Trichoderma T2 in combination with B. substilis UKM1 gave the highest reduction in pre-post damping off, disease infection and disease severity caused by M. grisea in greenhouse conditions. From the greenhouse experimentations, the biocontrol agents were more efficient in inhibiting rice blast when applied prior to transplantation as compared to when applications were made at seed setting stage.

AB - Rice blast causes yield losses to rice farmers worldwide. Although this problem is currently being addressed through the use of resistant rice varieties, fungicide and rotation farming, these methods alone do not form a durable, long lasting solution in mitigating disease. Here we obtained Trichoderma isolates from soil and tested their efficacy as biocontrol agents against Magnaporthe grisea. Twenty-two Trichoderma isolates were identified and used in dual culture assays to determine antagonistic ability before isolates showing promise were administered to rice plants grown in greenhouse conditions where the efficacy of these isolates was tested individually or as dual inoculums with Bacillus substilis UKM1. The results showed that the dual inoculation of biocontrol agents caused significant (p ≤0.05) inhibition of M. grisea as compared to a single agent. In addition, we observed the inhibitory effect of Trichoderma T2 under SEM where the hypha of the biocontrol agent coiled and possibly enzymatically degraded the pathogen. Trichoderma T2 in combination with B. substilis UKM1 gave the highest reduction in pre-post damping off, disease infection and disease severity caused by M. grisea in greenhouse conditions. From the greenhouse experimentations, the biocontrol agents were more efficient in inhibiting rice blast when applied prior to transplantation as compared to when applications were made at seed setting stage.

KW - Bacillus substilis UKM1

KW - Biocontrol

KW - Dual plate culture

KW - Hyphal coiling

KW - Magnaporthe grisea

KW - Trichoderma spp

UR - http://www.scopus.com/inward/record.url?scp=84918500417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84918500417&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84918500417

VL - 8

SP - 1324

EP - 1335

JO - Australian Journal of Crop Science

JF - Australian Journal of Crop Science

SN - 1835-2693

IS - 9

ER -