Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold

B. Hemabarathy Bharatham, Siti Fathiah Masre, Leo Hwee Xien, Nurnadiah Ahmad

Research output: Contribution to journalArticle

Abstract

In vivo stability of biomaterial-based bone scaffolds often present a significant drawback in the development of materials for tissue engineering purpose. Previously developed nanobiocomposite bone scaffold using alginate and nano cockle shell powder has shown ideal characteristics. However, it showed high degradation rate and reduced stability in an in vivo setting. In this study, we aim to observe the effect of cross-linking glutaraldehyde (GA) in three different concentrations of 0.5%, 1% and 2% during the fabrication process as a potential factor in increasing scaffold stability. Microstructure observations of scaffolds using scanning electron microscope (SEM) showed all scaffolds crossed linked with GA and control had an ideal pore size ranging from 166.8-203.5 µm. Increase in porosity compared to the control scaffolds was observed in scaffolds cross-linked with 2% GA which also presented better structural integrity as scored through semi-quantitative methods. Tested pH values during the degradation period showed that scaffolds from all groups remained within the range of 7.73-8.76. In vitro studies using osteoblast showed no significant changes in cell viability but a significant increase in ALP enzyme levels in scaffold cross-linked with 2% GA. The calcium content released from all scaffold showed significant differences within and between the groups. It can be concluded that the use of GA in the preparation stage of the scaffold did not affect the growth and proliferation of osteoblast and use of 2% GA showed improved scaffold structural integrity and porosity.

Original languageEnglish
Pages (from-to)2557-2563
Number of pages7
JournalSains Malaysiana
Volume47
Issue number10
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Glutaral
Bone and Bones
Porosity
Osteoblasts
Cardiidae
Biocompatible Materials
Tissue Engineering
Powders
Cell Survival
Electrons
Calcium
Enzymes
Growth

Keywords

  • Bone scaffold
  • Glutaraldehyde
  • Nanobiocomposite
  • Osteoblast

ASJC Scopus subject areas

  • General

Cite this

Evaluating physical and biological characteristics of glutaraldehyde (GA) cross-linked nano-biocomposite bone scaffold. / Bharatham, B. Hemabarathy; Masre, Siti Fathiah; Xien, Leo Hwee; Ahmad, Nurnadiah.

In: Sains Malaysiana, Vol. 47, No. 10, 01.10.2018, p. 2557-2563.

Research output: Contribution to journalArticle

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