Eurycoma longifolia, a malaysian medicinal herb, significantly upregulates proliferation and differentiation in pre-osteoblasts (MC3T3-E1)

An in Vitro model

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: Eurycoma longifolia (EL), a well-recognized Malaysian medicinal herb, has gained widespread popularity due to its ability to protect against bone calcium loss in androgen-deficient osteoporosis. Nevertheless numerous animal studies have proved the bone protective effect of EL; however, the exact mechanism is not well-explained yet. Thus, the present study was aimed to explore the in vitro basis of bone protective effects of EL by using mouse pre-osteoblast cell line (MC3T3-E1). Methods: The cytotoxicity and proliferative potential of EL were evaluated by lactate dehydrogenase (LDH) and cell counting methods. Despite cell growth, the ability of EL to promote osteogenic differentiation of bone-forming cells was assessed by quantifying collagen (early differentiation marker) and calcium (late differentiation marker) in EL-treated bone forming cells. Results: Resulting data obtained from dose optimization study revealed that EL at 5 to 50 μg/ml concentration showed marked effects in significantly promoting cell growth in MC3T3-E1 cells. As such, resulting data also demonstrated the superior potential of EL in up regulating collagen synthesis and mineralization (calcium deposition) in MC3T3-E1 cells at 25 μg/ml, in comparison to untreated (negative control) and dihydrotestosterone (5α-DHT)-treated cells (positive control). Conclusion: These pronounced effects of EL on osteoblasts provide an in vitro basis for the bone protective potential of EL and thus can be considered as an alternative regimen for the treatment of androgen-deficient male osteoporosis.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume8
Issue number11
DOIs
Publication statusPublished - 2016

Fingerprint

Eurycoma
Medicinal Plants
Osteoblasts
Up-Regulation
Bone and Bones
Differentiation Antigens
Calcium
Androgens
Osteoporosis
Collagen
In Vitro Techniques
Dihydrotestosterone
Growth
L-Lactate Dehydrogenase

Keywords

  • 5α-dihydrotestosterone
  • Androgen-deficient osteoporosis
  • Cell proliferation
  • Collagen deposition
  • Eurycoma longifolia
  • Mineralization

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

@article{17102a8dc028411190eb939eb84cb961,
title = "Eurycoma longifolia, a malaysian medicinal herb, significantly upregulates proliferation and differentiation in pre-osteoblasts (MC3T3-E1): An in Vitro model",
abstract = "Objective: Eurycoma longifolia (EL), a well-recognized Malaysian medicinal herb, has gained widespread popularity due to its ability to protect against bone calcium loss in androgen-deficient osteoporosis. Nevertheless numerous animal studies have proved the bone protective effect of EL; however, the exact mechanism is not well-explained yet. Thus, the present study was aimed to explore the in vitro basis of bone protective effects of EL by using mouse pre-osteoblast cell line (MC3T3-E1). Methods: The cytotoxicity and proliferative potential of EL were evaluated by lactate dehydrogenase (LDH) and cell counting methods. Despite cell growth, the ability of EL to promote osteogenic differentiation of bone-forming cells was assessed by quantifying collagen (early differentiation marker) and calcium (late differentiation marker) in EL-treated bone forming cells. Results: Resulting data obtained from dose optimization study revealed that EL at 5 to 50 μg/ml concentration showed marked effects in significantly promoting cell growth in MC3T3-E1 cells. As such, resulting data also demonstrated the superior potential of EL in up regulating collagen synthesis and mineralization (calcium deposition) in MC3T3-E1 cells at 25 μg/ml, in comparison to untreated (negative control) and dihydrotestosterone (5α-DHT)-treated cells (positive control). Conclusion: These pronounced effects of EL on osteoblasts provide an in vitro basis for the bone protective potential of EL and thus can be considered as an alternative regimen for the treatment of androgen-deficient male osteoporosis.",
keywords = "5α-dihydrotestosterone, Androgen-deficient osteoporosis, Cell proliferation, Collagen deposition, Eurycoma longifolia, Mineralization",
author = "Thu, {Hnin Ei} and {Naina Mohamed}, Isa and Zahid Hussain and Norazlina Mohamed and Shuid, {Ahmad Nazrun}",
year = "2016",
doi = "10.22159/ijpps.2016v8i11.14518",
language = "English",
volume = "8",
pages = "199--204",
journal = "International Journal of Pharmacy and Pharmaceutical Sciences",
issn = "0975-1491",
publisher = "IJPPS",
number = "11",

}

TY - JOUR

T1 - Eurycoma longifolia, a malaysian medicinal herb, significantly upregulates proliferation and differentiation in pre-osteoblasts (MC3T3-E1)

T2 - An in Vitro model

AU - Thu, Hnin Ei

AU - Naina Mohamed, Isa

AU - Hussain, Zahid

AU - Mohamed, Norazlina

AU - Shuid, Ahmad Nazrun

PY - 2016

Y1 - 2016

N2 - Objective: Eurycoma longifolia (EL), a well-recognized Malaysian medicinal herb, has gained widespread popularity due to its ability to protect against bone calcium loss in androgen-deficient osteoporosis. Nevertheless numerous animal studies have proved the bone protective effect of EL; however, the exact mechanism is not well-explained yet. Thus, the present study was aimed to explore the in vitro basis of bone protective effects of EL by using mouse pre-osteoblast cell line (MC3T3-E1). Methods: The cytotoxicity and proliferative potential of EL were evaluated by lactate dehydrogenase (LDH) and cell counting methods. Despite cell growth, the ability of EL to promote osteogenic differentiation of bone-forming cells was assessed by quantifying collagen (early differentiation marker) and calcium (late differentiation marker) in EL-treated bone forming cells. Results: Resulting data obtained from dose optimization study revealed that EL at 5 to 50 μg/ml concentration showed marked effects in significantly promoting cell growth in MC3T3-E1 cells. As such, resulting data also demonstrated the superior potential of EL in up regulating collagen synthesis and mineralization (calcium deposition) in MC3T3-E1 cells at 25 μg/ml, in comparison to untreated (negative control) and dihydrotestosterone (5α-DHT)-treated cells (positive control). Conclusion: These pronounced effects of EL on osteoblasts provide an in vitro basis for the bone protective potential of EL and thus can be considered as an alternative regimen for the treatment of androgen-deficient male osteoporosis.

AB - Objective: Eurycoma longifolia (EL), a well-recognized Malaysian medicinal herb, has gained widespread popularity due to its ability to protect against bone calcium loss in androgen-deficient osteoporosis. Nevertheless numerous animal studies have proved the bone protective effect of EL; however, the exact mechanism is not well-explained yet. Thus, the present study was aimed to explore the in vitro basis of bone protective effects of EL by using mouse pre-osteoblast cell line (MC3T3-E1). Methods: The cytotoxicity and proliferative potential of EL were evaluated by lactate dehydrogenase (LDH) and cell counting methods. Despite cell growth, the ability of EL to promote osteogenic differentiation of bone-forming cells was assessed by quantifying collagen (early differentiation marker) and calcium (late differentiation marker) in EL-treated bone forming cells. Results: Resulting data obtained from dose optimization study revealed that EL at 5 to 50 μg/ml concentration showed marked effects in significantly promoting cell growth in MC3T3-E1 cells. As such, resulting data also demonstrated the superior potential of EL in up regulating collagen synthesis and mineralization (calcium deposition) in MC3T3-E1 cells at 25 μg/ml, in comparison to untreated (negative control) and dihydrotestosterone (5α-DHT)-treated cells (positive control). Conclusion: These pronounced effects of EL on osteoblasts provide an in vitro basis for the bone protective potential of EL and thus can be considered as an alternative regimen for the treatment of androgen-deficient male osteoporosis.

KW - 5α-dihydrotestosterone

KW - Androgen-deficient osteoporosis

KW - Cell proliferation

KW - Collagen deposition

KW - Eurycoma longifolia

KW - Mineralization

UR - http://www.scopus.com/inward/record.url?scp=84995503418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84995503418&partnerID=8YFLogxK

U2 - 10.22159/ijpps.2016v8i11.14518

DO - 10.22159/ijpps.2016v8i11.14518

M3 - Article

VL - 8

SP - 199

EP - 204

JO - International Journal of Pharmacy and Pharmaceutical Sciences

JF - International Journal of Pharmacy and Pharmaceutical Sciences

SN - 0975-1491

IS - 11

ER -