Tumbuhan etnoperubatan sebagai perumah aktinomiset endofit yang bersifat bioaktif

Translated title of the contribution: Ethnomedicinal plants as host of bioactive endophytic actinomycetes

Nurul Izzah Mohd Sarmin, Noraziah Mohamad Zin, Ng Kim Tien, Nik Marzuki Sidik, Onuma Kaewkla, Christopher M M Franco

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nine endophytic actinomycetes have been isolated from medicinal plants in several parts of the Peninsular Malaysia. The isolates were identified by morphological observation, amplification of 16S rRNA genes and 16S rRNA sequencing analysis. Preliminary screenings of antimicrobial activities were measured by streak plate method. The presence of substrate and aerial mycelium, mass spore colour, diffusible pigment and spore chain morphology of all isolates were identical to Streptomyces sp. and Microbispora sp. The phylogenetic tree of partial sequence of 16S rRNA showed that isolate SUK 08, SUK 10 and SUK 15 were closely related to Streptomyces eurythermus ATCC 14975T but yet they were isolated from different plants. All isolates demonstrated antimicrobial activities against tested bacteria and fungi. Four active isolates namely SUK 08, SUK 10, SUK 12 and SUK 15, have killing and inhibition activities up to 100% against one or more pathogenic organism such as Bacillus subtilis, Aspergillus fumigatus, Aspergillus niger, Fusarium solani, Rhizoctonia solani and Trichoderma viride. These results confirmed that ethnomedicinal plants harbor bioactive endophytic Actinomycetes which should represent as a novel source in the search of antibacterial and antimicotic agents.

Original languageUndefined/Unknown
Pages (from-to)547-551
Number of pages5
JournalSains Malaysiana
Volume41
Issue number5
Publication statusPublished - May 2012

Fingerprint

Actinobacteria
ribosomal RNA
Streptomyces eurythermus
Microbispora
spores
anti-infective agents
Trichoderma viride
Fusarium solani
Aspergillus fumigatus
Streptomyces
Thanatephorus cucumeris
Aspergillus niger
Bacillus subtilis
Malaysia
plant anatomy
mycelium
medicinal plants
pigments
screening
fungi

Keywords

  • 16S rRNA
  • Actinomycetes
  • Endophytes
  • Ethnomedicinal plant

ASJC Scopus subject areas

  • General

Cite this

Sarmin, N. I. M., Mohamad Zin, N., Tien, N. K., Sidik, N. M., Kaewkla, O., & Franco, C. M. M. (2012). Tumbuhan etnoperubatan sebagai perumah aktinomiset endofit yang bersifat bioaktif. Sains Malaysiana, 41(5), 547-551.

Tumbuhan etnoperubatan sebagai perumah aktinomiset endofit yang bersifat bioaktif. / Sarmin, Nurul Izzah Mohd; Mohamad Zin, Noraziah; Tien, Ng Kim; Sidik, Nik Marzuki; Kaewkla, Onuma; Franco, Christopher M M.

In: Sains Malaysiana, Vol. 41, No. 5, 05.2012, p. 547-551.

Research output: Contribution to journalArticle

Sarmin, NIM, Mohamad Zin, N, Tien, NK, Sidik, NM, Kaewkla, O & Franco, CMM 2012, 'Tumbuhan etnoperubatan sebagai perumah aktinomiset endofit yang bersifat bioaktif', Sains Malaysiana, vol. 41, no. 5, pp. 547-551.
Sarmin NIM, Mohamad Zin N, Tien NK, Sidik NM, Kaewkla O, Franco CMM. Tumbuhan etnoperubatan sebagai perumah aktinomiset endofit yang bersifat bioaktif. Sains Malaysiana. 2012 May;41(5):547-551.
Sarmin, Nurul Izzah Mohd ; Mohamad Zin, Noraziah ; Tien, Ng Kim ; Sidik, Nik Marzuki ; Kaewkla, Onuma ; Franco, Christopher M M. / Tumbuhan etnoperubatan sebagai perumah aktinomiset endofit yang bersifat bioaktif. In: Sains Malaysiana. 2012 ; Vol. 41, No. 5. pp. 547-551.
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