Estrogenic activity of selected Myrsinaceae species in MCF-7 human breast cancer cells

Jamia Azdina Jamal, Noorlela Ramli, Johnson Stanslas, Khairana Husain

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Eight Myrsinaceae species (Ardisia kunstleri King & Gamble, A. oxyphylla Wall., A. villosa Roxb., Embelia coriacea Wall., Labisia pumila (Blume) Mez var. alata (purple-leafed), L. pumila (Blume) Mez var. alata (green-leafed), Maesa ramentacea Wall. and Rapanea porteriana (Wall. ex A.DC.) Mez) were investigated for their estrogenic activity in the estrogen-sensitive MCF-7 human breast cancer cells. A total of 30 extracts of different plant parts (leaves, twigs and roots) comprising of methanol (14) and water (16) extracts were assayed for cell proliferation activity at a concentration range of between 10 -10 and 10 -4 g/mL. 17β-Estradiol (10 -9 M) was used as a positive control. The aqueous extract of M. ramentaceae leaves showed the highest cell proliferation activity (136.9±2.5% at 10 -5 g/mL, p<0.01 compared to estrogen-free solvent control, 100%) with an RPE value of 121.6% comparative to 17β-estradiol (RPE = 100%), followed by the aqueous leaf extract of L. pumila var. alata (purple-leafed) (131.0±2.9% at 10 -8 g/mL, p<0.01; RPE = 83.1%). The methanol extracts of A. oxyphylla roots and M. ramentacea leaves, and the aqueous leaf extracts of R. porteriana were found to significantly stimulate the proliferation of MCF-7 cells. The results may suggest that the Myrsinaceae species can potentially be explored for estrogenic activity and for sources of phytoestrogens.

Original languageEnglish
Pages (from-to)547-553
Number of pages7
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume4
Issue numberSUPPL. 4
Publication statusPublished - Sep 2012

Fingerprint

Myrsinaceae
Breast Neoplasms
Methanol
Embelia
Ardisia
Estradiol
Estrogens
Cell Proliferation
Phytoestrogens
Plant Roots
Plant Leaves
MCF-7 Cells
Water

Keywords

  • Labisia pumila var. alata
  • Maesa ramentacea
  • MCF-7 proliferation assay
  • Relative proliferative effect

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Estrogenic activity of selected Myrsinaceae species in MCF-7 human breast cancer cells. / Jamal, Jamia Azdina; Ramli, Noorlela; Stanslas, Johnson; Husain, Khairana.

In: International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 4, No. SUPPL. 4, 09.2012, p. 547-553.

Research output: Contribution to journalArticle

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abstract = "Eight Myrsinaceae species (Ardisia kunstleri King & Gamble, A. oxyphylla Wall., A. villosa Roxb., Embelia coriacea Wall., Labisia pumila (Blume) Mez var. alata (purple-leafed), L. pumila (Blume) Mez var. alata (green-leafed), Maesa ramentacea Wall. and Rapanea porteriana (Wall. ex A.DC.) Mez) were investigated for their estrogenic activity in the estrogen-sensitive MCF-7 human breast cancer cells. A total of 30 extracts of different plant parts (leaves, twigs and roots) comprising of methanol (14) and water (16) extracts were assayed for cell proliferation activity at a concentration range of between 10 -10 and 10 -4 g/mL. 17β-Estradiol (10 -9 M) was used as a positive control. The aqueous extract of M. ramentaceae leaves showed the highest cell proliferation activity (136.9±2.5{\%} at 10 -5 g/mL, p<0.01 compared to estrogen-free solvent control, 100{\%}) with an RPE value of 121.6{\%} comparative to 17β-estradiol (RPE = 100{\%}), followed by the aqueous leaf extract of L. pumila var. alata (purple-leafed) (131.0±2.9{\%} at 10 -8 g/mL, p<0.01; RPE = 83.1{\%}). The methanol extracts of A. oxyphylla roots and M. ramentacea leaves, and the aqueous leaf extracts of R. porteriana were found to significantly stimulate the proliferation of MCF-7 cells. The results may suggest that the Myrsinaceae species can potentially be explored for estrogenic activity and for sources of phytoestrogens.",
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