Estimation of genetic diversity and identification of potential rice lines for two-line hybrid based on microsatellite marker and phenotypic trait analyses

M. Nur Azreen, S. Siju, M. A R Bhuiyan, R Wickneswari V Ratnam

Research output: Contribution to journalArticle

Abstract

The genetic relatedness among 31 advanced breeding lines derived from crosses between O. rufipogon and MR219, three Malaysian rice varieties (MR219, MR253 and MR263) and two thermosensitive genic male sterile (TGMS) lines (IR73827- 23-26-15-7S and IR77271–42–5–4–36S) were determined using different microsatellite (SSR) markers. A total of 81 alleles were detected with the 26 SSR markers, with an average of 3.12 alleles per locus and a PIC value varying from 0.028 to 0.450. UPGMA cluster analysis separated the entire accessions into seven major groups. Group I accommodated twenty two advanced breeding lines along with the parental variety, MR219. Eight advanced breeding lines formed the Group III while Group II, IV, V, VI and VII contained either a single improved line or variety. Group II was constituted by the improved line G33, while Group IV and V were constituted by the released varieties MR253 and MR263, respectively. On the other hand, two TGMS lines viz. IR73827-23-26-15-7S and IR77271–42–5–4–36S were clustered under Group VI and Group VII, respectively. Based on the genetic distance (GD) data derived from the analysis, three combinations with maximum GD viz., MR253 × G33 (GD= 0.61), MR253 × G02 (GD= 0.61) and MR253 × G16 (GD=0.56) are suggested to be used as parental lines in two line hybrid rice breeding system. Besides GD, the suggested lines possess promising yield and yield related traits. The released variety, MR253 is suggested to be developed as a TGMS line. These suggested parental lines are expected to produce highest hybrid vigour and will be useful for future breeding programmes.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalMalaysian Applied Biology
Volume43
Issue number2
Publication statusPublished - 1 Dec 2014

Fingerprint

Microsatellite Repeats
Breeding
genetic distance
microsatellite repeats
rice
genetic variation
breeding lines
Alleles
Hybrid Vigor
alleles
breeding
Cluster Analysis
heterosis
genetic relationships
Oryza
cluster analysis
loci

Keywords

  • Agronomic traits
  • Cluster analysis
  • Genetic distance
  • Heterosis
  • Microsatellite markers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Estimation of genetic diversity and identification of potential rice lines for two-line hybrid based on microsatellite marker and phenotypic trait analyses. / Nur Azreen, M.; Siju, S.; Bhuiyan, M. A R; V Ratnam, R Wickneswari.

In: Malaysian Applied Biology, Vol. 43, No. 2, 01.12.2014, p. 81-88.

Research output: Contribution to journalArticle

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AB - The genetic relatedness among 31 advanced breeding lines derived from crosses between O. rufipogon and MR219, three Malaysian rice varieties (MR219, MR253 and MR263) and two thermosensitive genic male sterile (TGMS) lines (IR73827- 23-26-15-7S and IR77271–42–5–4–36S) were determined using different microsatellite (SSR) markers. A total of 81 alleles were detected with the 26 SSR markers, with an average of 3.12 alleles per locus and a PIC value varying from 0.028 to 0.450. UPGMA cluster analysis separated the entire accessions into seven major groups. Group I accommodated twenty two advanced breeding lines along with the parental variety, MR219. Eight advanced breeding lines formed the Group III while Group II, IV, V, VI and VII contained either a single improved line or variety. Group II was constituted by the improved line G33, while Group IV and V were constituted by the released varieties MR253 and MR263, respectively. On the other hand, two TGMS lines viz. IR73827-23-26-15-7S and IR77271–42–5–4–36S were clustered under Group VI and Group VII, respectively. Based on the genetic distance (GD) data derived from the analysis, three combinations with maximum GD viz., MR253 × G33 (GD= 0.61), MR253 × G02 (GD= 0.61) and MR253 × G16 (GD=0.56) are suggested to be used as parental lines in two line hybrid rice breeding system. Besides GD, the suggested lines possess promising yield and yield related traits. The released variety, MR253 is suggested to be developed as a TGMS line. These suggested parental lines are expected to produce highest hybrid vigour and will be useful for future breeding programmes.

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