Establishment of an immunofluorescence assay to detect IgM antibodies to Nipah virus using HeLa cells expressing recombinant nucleoprotein

Yoshihiro Kaku, Eun sil Park, Akira Noguchi, Satoshi Inoue, Ross Lunt, Fedelino F. Malbas, Catalino Demetria, Hui Min Neoh, A. Rahman A. Jamal, Shigeru Morikawa

Research output: Contribution to journalArticle

Abstract

A novel indirect fluorescent antibody test (IFAT) for detection of IgM against Nipah virus (NiV) was developed using HeLa 229 cells expressing recombinant NiV nucleocapsid protein (NiV-N). The NiV IFAT was evaluated using three panels of sera: a) experimentally produced sera from NiV-N-immunized/pre-immunized macaques, b) post-infection human sera associated with a Nipah disease outbreak in the Philippines in 2014, and c) human sera from a non-exposed Malaysian population. Immunized macaque sera showed a characteristic granular staining pattern of the NiV-N expressed antigen in HeLa 229 cells, which was readily distinguished from negative-binding results of the pre-immunized macaque sera. The IgM antibody titers in sequential serum samples (n = 7) obtained from three Nipah patients correlated well with previously published results using conventional IgM capture ELISA and SNT serology. The 90 human serum samples from unexposed persons were unreactive by IFAT. The IFAT utilizing NiV-N-expressing HeLa 229 cells to detect IgM antibody in an early stage of NiV infection is an effective approach, which could be utilized readily in local laboratories to complement other capabilities in NiV-affected countries.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalJournal of Virological Methods
Volume269
DOIs
Publication statusPublished - 1 Jul 2019

Fingerprint

Nipah Virus
Nucleoproteins
HeLa Cells
Fluorescent Antibody Technique
Immunoglobulin M
Antibodies
Serum
Macaca
Nucleocapsid Proteins
Philippines
Virus Diseases
Serology
Disease Outbreaks
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Antigens

Keywords

  • IgM
  • Nipah virus
  • Serological assay

ASJC Scopus subject areas

  • Virology

Cite this

Establishment of an immunofluorescence assay to detect IgM antibodies to Nipah virus using HeLa cells expressing recombinant nucleoprotein. / Kaku, Yoshihiro; Park, Eun sil; Noguchi, Akira; Inoue, Satoshi; Lunt, Ross; Malbas, Fedelino F.; Demetria, Catalino; Neoh, Hui Min; A. Jamal, A. Rahman; Morikawa, Shigeru.

In: Journal of Virological Methods, Vol. 269, 01.07.2019, p. 83-87.

Research output: Contribution to journalArticle

Kaku, Yoshihiro ; Park, Eun sil ; Noguchi, Akira ; Inoue, Satoshi ; Lunt, Ross ; Malbas, Fedelino F. ; Demetria, Catalino ; Neoh, Hui Min ; A. Jamal, A. Rahman ; Morikawa, Shigeru. / Establishment of an immunofluorescence assay to detect IgM antibodies to Nipah virus using HeLa cells expressing recombinant nucleoprotein. In: Journal of Virological Methods. 2019 ; Vol. 269. pp. 83-87.
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