Enzyme inhibitory and immunomodulatory activities of the depsidone lobaric acid extracted from the lichen Heterodermia sp

Vinitha M. Thadhani, Qamar Naaz, M. Iqbal Choudhary, Osman Mesaik Mohammed Ahmed Hassan, Veranja Karunaratne

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study evaluates the enzyme inhibitory and immunomodulatory activities of the lichen specific depsidone, lobaric acid. Lobaric acid was extracted with methanol from Heterodermia sp. found in Labukelle, Sri Lanka with a yield of 0.67 %. It was subjected to enzyme inhibition assays using acetyl and butyryl-cholinesterase, phosphodiesterase, β-glucuronidase, α-glucosidase and urease. In the β - glucuronidase inhibitory activity it showed an IC50 value of 3.28 ± 0.05 μM, which was significantly lower than that of the standard, D-saccharic acid 1, 4-lactone (IC50 = 48.4 ± 1.2 μM). Lobaric acid showed a significant inhibition of phosphodiesterase enzyme with an IC50 = 313.7 ± 2.2, when compared with the two standards EDTA (IC50 = 274.0 ± 0.1 μM) and cysteine (IC50 = 748.0 ± 0.1 μM). Lobaric acid showed a moderate actyl and butyryl -cholinesterase inhibitory activity while it showed no activity against the enzymes α-glucosidase and urease. In the immunomodulatory assay, lobaric acid exhibited a potent oxidative burst inhibitory activity in human polymorphonuclear (PMN) cells. It suppressed both the myloperozidase dependant and myloperozidase independent reactive oxygen species (ROS) production of PMNs. The results indicate the pharmacological potential of lobaric acid as a lead compound for further studies.

Original languageEnglish
Pages (from-to)193-196
Number of pages4
JournalJournal of the National Science Foundation of Sri Lanka
Volume42
Issue number2
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Enzymes
Glucosidases
Urease
Glucuronidase
Cholinesterases
Phosphoric Diester Hydrolases
Assays
Lead compounds
Enzyme inhibition
Edetic Acid
Cysteine
Methanol
Lichens
lobaric acid
depsidone
Reactive Oxygen Species

Keywords

  • Enzyme inhibitory activity
  • Heterodermia sp.
  • Immunomodulatory activity
  • Lobaric acid
  • Phosphodiesterase
  • β-glucuronidase

ASJC Scopus subject areas

  • General

Cite this

Enzyme inhibitory and immunomodulatory activities of the depsidone lobaric acid extracted from the lichen Heterodermia sp. / Thadhani, Vinitha M.; Naaz, Qamar; Iqbal Choudhary, M.; Mohammed Ahmed Hassan, Osman Mesaik; Karunaratne, Veranja.

In: Journal of the National Science Foundation of Sri Lanka, Vol. 42, No. 2, 2014, p. 193-196.

Research output: Contribution to journalArticle

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