Enzyme immunoassay for the detection of antibody to Pseudomonas pseudomallei exotoxin in mice

G. Ismail, Mohammed Noor Embi, O. Omar, N. Razak, J. C. Allen, C. J. Smith

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An enzyme immunoassay (enzyme-linked immunosorbent assay; ELISA) to detect mouse antibody to exotoxin of Pseudomonas pseudomallei was developed in which exotoxin preparations were used to coat the solid phase. The specificity of the assay was supported by inhibition assays with the toxin preparations. Sera from immunised mice and supernatants from antitoxin-producing hybridoma cells were tested by this technique, and the ELISA described appears to provide a sensitive, specific, and practical method for the determination of (a) antibody to exotoxin, and (b) concentration of exotoxin. Both systems may prove invaluable in the diagnosis of melioidosis and in epidemiological studies of melioidosis in endemic areas.

Original languageEnglish
Pages (from-to)27-31
Number of pages5
JournalFEMS Microbiology Letters
Volume40
Issue number1
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Burkholderia pseudomallei
Exotoxins
Immunoenzyme Techniques
Melioidosis
Antibodies
Enzyme-Linked Immunosorbent Assay
Antitoxins
Hybridomas
Epidemiologic Studies
Serum

Keywords

  • (Detection
  • Antibody
  • Enzyme immunoassay)
  • Exotoxin
  • Pseudomonas pseudomallei

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Ismail, G., Embi, M. N., Omar, O., Razak, N., Allen, J. C., & Smith, C. J. (1987). Enzyme immunoassay for the detection of antibody to Pseudomonas pseudomallei exotoxin in mice. FEMS Microbiology Letters, 40(1), 27-31.

Enzyme immunoassay for the detection of antibody to Pseudomonas pseudomallei exotoxin in mice. / Ismail, G.; Embi, Mohammed Noor; Omar, O.; Razak, N.; Allen, J. C.; Smith, C. J.

In: FEMS Microbiology Letters, Vol. 40, No. 1, 1987, p. 27-31.

Research output: Contribution to journalArticle

Ismail, G, Embi, MN, Omar, O, Razak, N, Allen, JC & Smith, CJ 1987, 'Enzyme immunoassay for the detection of antibody to Pseudomonas pseudomallei exotoxin in mice', FEMS Microbiology Letters, vol. 40, no. 1, pp. 27-31.
Ismail, G. ; Embi, Mohammed Noor ; Omar, O. ; Razak, N. ; Allen, J. C. ; Smith, C. J. / Enzyme immunoassay for the detection of antibody to Pseudomonas pseudomallei exotoxin in mice. In: FEMS Microbiology Letters. 1987 ; Vol. 40, No. 1. pp. 27-31.
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