Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase

Effects of reaction conditions for optimum hemicellulose hydrolysis

Nur Izyan Wan Azelee, Jamaliah Md Jahim, Ahmad Fauzi Ismail, Siti Fatimah Zaharah Mohamad Fuzi, Roshanida A. Rahman, Nazlee Faisal Ghazali, Rosli Md Illias

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of various ranges of reaction conditions (substrate loading, xylanase loading, pH, temperature and time) were systematically and thoroughly studied. The study of hemicellulosic kenaf conversion using single enzyme was proven to effectively yield up to 59% of conversion after 48 h incubation using 3% (w/v) of substrate loading and 400 U of xylanase loading at 50°C in pH 4.0 buffer systems. The use of a single xylanase on the pre-treated kenaf was a key parameter in understanding the action of the main hemicellulose degrading enzyme towards hydrolysing the hemicellulose backbone of the pre-treated kenaf. Enzyme feeding strategy was also conducted and the results revealed that a batch feeding strategy of 400 U xylanase loading yielded the highest hemicellulose hydrolysis compared to the other fed batch enzyme feeding strategy. It was concluded that each reaction conditions and enzyme feeding strategy gave a significant impact towards the enzymatic hydrolysis and the use of optimum reaction conditions resulted in a high product yield at the end of the reaction. With the discovery of the importance of each reaction conditions involved in a hydrolysis, this study highlights the need for a comprehensive investigation on the reaction conditions using a mixture of enzymes towards achieving a complete hydrolysis with a higher product yield.

Original languageEnglish
Pages (from-to)54-66
Number of pages13
JournalAmerican Journal of Agricultural and Biological Science
Volume11
Issue number2
DOIs
Publication statusPublished - 28 Apr 2016

Fingerprint

Hibiscus
kenaf
xylanases
enzymatic hydrolysis
hemicellulose
feeding methods
Hydrolysis
hydrolysis
enzymes
Enzymes
xylooligosaccharides
Hibiscus cannabinus
food additives
Food Additives
functional foods
buffers
Dietary Supplements
industry
Industry
Buffers

Keywords

  • Enzymatic hydrolysis
  • Hemicellulose
  • Kenaf
  • Reaction conditions
  • Xylanase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase : Effects of reaction conditions for optimum hemicellulose hydrolysis. / Wan Azelee, Nur Izyan; Md Jahim, Jamaliah; Ismail, Ahmad Fauzi; Fuzi, Siti Fatimah Zaharah Mohamad; Rahman, Roshanida A.; Ghazali, Nazlee Faisal; Illias, Rosli Md.

In: American Journal of Agricultural and Biological Science, Vol. 11, No. 2, 28.04.2016, p. 54-66.

Research output: Contribution to journalArticle

Wan Azelee, Nur Izyan ; Md Jahim, Jamaliah ; Ismail, Ahmad Fauzi ; Fuzi, Siti Fatimah Zaharah Mohamad ; Rahman, Roshanida A. ; Ghazali, Nazlee Faisal ; Illias, Rosli Md. / Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase : Effects of reaction conditions for optimum hemicellulose hydrolysis. In: American Journal of Agricultural and Biological Science. 2016 ; Vol. 11, No. 2. pp. 54-66.
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