Gliserolisis berenzim oleh metil laurat menggunakan Lipase b Candida antarctica

Translated title of the contribution: Enzymatic glycerolysis of methyl laurate utilizing Candida Antarctica lipase b

Norul Naziraa Ahmad Jamlus, Jumat Salimon, Darfizzi Derawi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Enzymatic glycerolysis using Candida antarctica lipase b (CALB) as catalyst was studied. Production of monolaurin was optimized based on two selected types of CALB, varying enzyme concentrations and reaction time. Novozyme-435 (N-435) and Aspergillus oryzae were used in this study. About 47.6% of monolaurin was synthesized using N-435 at a 5% enzyme concentration within 24 hours of reaction time. Characterizations were performed using different techniques namely gas chromatography-flame ionization detector (GC-FID), thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) analysis.

Original languageMalay
Pages (from-to)1365-1372
Number of pages8
JournalMalaysian Journal of Analytical Sciences
Volume20
Issue number6
DOIs
Publication statusPublished - 2016

Fingerprint

Candida
Lipase
Thin layer chromatography
Aspergillus
Enzymes
Gas chromatography
Ionization
Detectors
Catalysts
monolaurin
lauric acid methyl ester
Novozyme 435

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Gliserolisis berenzim oleh metil laurat menggunakan Lipase b Candida antarctica. / Jamlus, Norul Naziraa Ahmad; Salimon, Jumat; Derawi, Darfizzi.

In: Malaysian Journal of Analytical Sciences, Vol. 20, No. 6, 2016, p. 1365-1372.

Research output: Contribution to journalArticle

@article{4e34b4db3f7c45f187a45e65894c8363,
title = "Gliserolisis berenzim oleh metil laurat menggunakan Lipase b Candida antarctica",
abstract = "Enzymatic glycerolysis using Candida antarctica lipase b (CALB) as catalyst was studied. Production of monolaurin was optimized based on two selected types of CALB, varying enzyme concentrations and reaction time. Novozyme-435 (N-435) and Aspergillus oryzae were used in this study. About 47.6{\%} of monolaurin was synthesized using N-435 at a 5{\%} enzyme concentration within 24 hours of reaction time. Characterizations were performed using different techniques namely gas chromatography-flame ionization detector (GC-FID), thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) analysis.",
keywords = "Aspergillus oryzae, Enzymatic glycerolysis, Methyl laurate, Monolaurin, Novozyme-435",
author = "Jamlus, {Norul Naziraa Ahmad} and Jumat Salimon and Darfizzi Derawi",
year = "2016",
doi = "10.17576/mjas-2016-2006-15",
language = "Malay",
volume = "20",
pages = "1365--1372",
journal = "Malaysian Journal of Analytical Sciences",
issn = "1394-2506",
publisher = "Faculty of Science and Technology, Universiti Kebangsaan Malaysia",
number = "6",

}

TY - JOUR

T1 - Gliserolisis berenzim oleh metil laurat menggunakan Lipase b Candida antarctica

AU - Jamlus, Norul Naziraa Ahmad

AU - Salimon, Jumat

AU - Derawi, Darfizzi

PY - 2016

Y1 - 2016

N2 - Enzymatic glycerolysis using Candida antarctica lipase b (CALB) as catalyst was studied. Production of monolaurin was optimized based on two selected types of CALB, varying enzyme concentrations and reaction time. Novozyme-435 (N-435) and Aspergillus oryzae were used in this study. About 47.6% of monolaurin was synthesized using N-435 at a 5% enzyme concentration within 24 hours of reaction time. Characterizations were performed using different techniques namely gas chromatography-flame ionization detector (GC-FID), thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) analysis.

AB - Enzymatic glycerolysis using Candida antarctica lipase b (CALB) as catalyst was studied. Production of monolaurin was optimized based on two selected types of CALB, varying enzyme concentrations and reaction time. Novozyme-435 (N-435) and Aspergillus oryzae were used in this study. About 47.6% of monolaurin was synthesized using N-435 at a 5% enzyme concentration within 24 hours of reaction time. Characterizations were performed using different techniques namely gas chromatography-flame ionization detector (GC-FID), thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) analysis.

KW - Aspergillus oryzae

KW - Enzymatic glycerolysis

KW - Methyl laurate

KW - Monolaurin

KW - Novozyme-435

UR - http://www.scopus.com/inward/record.url?scp=85003481211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85003481211&partnerID=8YFLogxK

U2 - 10.17576/mjas-2016-2006-15

DO - 10.17576/mjas-2016-2006-15

M3 - Article

VL - 20

SP - 1365

EP - 1372

JO - Malaysian Journal of Analytical Sciences

JF - Malaysian Journal of Analytical Sciences

SN - 1394-2506

IS - 6

ER -