Enzymatic degradation of lignin extracted from oil palm empty fruit bunch using laccase and cutinase

Satriani Aga Pasma, Rusli Daik, Suria Ramli, Mohamad Yusof Maskat, Mohd Hanif Zulfakar

Research output: Contribution to journalArticle

Abstract

Enzymatic degradation products of lignin, having potential for added value, were obtained by extraction and subsequent enzymatic treatments of oil palm empty fruit bunch (OPEFB). The objective was to optimize the production of OPEFB lignin degradation products and study the effects of different enzymes and reaction media. Powder of OPEFB lignin was recovered from organsolv black liquor by using methanol, acidified water, and deionized water, respectively. OPEFB lignin was later subjected to enzymatic hydrolysis in an incubator shaker for 24 h using laccase and cutinase in various reaction media, including phenol, water, and acetate buffer. Nine compounds were recovered as OPEFB lignin degradation products, namely hydroxybenzoic acid, hydroxybenzaldehyde, vanillic acid, vanillin, syringic acid, syringaldehyde, coumaric acid, ferulic acid, and guaiacyl alcohol. When laccase was used in water, the product with the highest concentration was syringaldehyde (4061.1 ± 89.9 mg/L), and followed by hydroxybenzoic acid (1029.8 ± 50.2 mg/L). Vanillic acid was the product with the highest concentration (126 ± 97.5 g/L) found when laccase was used in phenol. When cutinase was used in water, products with the highest concentrations in the medium were syringaldehyde (4837.6 ± 156.4 mg/L) and syringic acid (2387.7 ± 105.3 mg/L). High performance liquid chromatography (HPLC) was used to quantify the OPEFB lignin degradation products.

Original languageEnglish
Pages (from-to)8879-8891
Number of pages13
JournalBioResources
Volume14
Issue number4
Publication statusPublished - 1 Jan 2019

Fingerprint

Laccase
Palm oil
Lignin
Fruits
lignin
fruit
Degradation
degradation
Acids
oil
acid
Vanillic Acid
Hydroxybenzoates
ferulic acid
Water
Phenol
Phenols
phenol
Coumaric Acids
Enzymatic hydrolysis

Keywords

  • Biodegradable
  • Biotransformation
  • Cellulose
  • Laccase
  • Lignin
  • Renewable

ASJC Scopus subject areas

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Cite this

Pasma, S. A., Daik, R., Ramli, S., Maskat, M. Y., & Zulfakar, M. H. (2019). Enzymatic degradation of lignin extracted from oil palm empty fruit bunch using laccase and cutinase. BioResources, 14(4), 8879-8891.

Enzymatic degradation of lignin extracted from oil palm empty fruit bunch using laccase and cutinase. / Pasma, Satriani Aga; Daik, Rusli; Ramli, Suria; Maskat, Mohamad Yusof; Zulfakar, Mohd Hanif.

In: BioResources, Vol. 14, No. 4, 01.01.2019, p. 8879-8891.

Research output: Contribution to journalArticle

Pasma, SA, Daik, R, Ramli, S, Maskat, MY & Zulfakar, MH 2019, 'Enzymatic degradation of lignin extracted from oil palm empty fruit bunch using laccase and cutinase', BioResources, vol. 14, no. 4, pp. 8879-8891.
Pasma, Satriani Aga ; Daik, Rusli ; Ramli, Suria ; Maskat, Mohamad Yusof ; Zulfakar, Mohd Hanif. / Enzymatic degradation of lignin extracted from oil palm empty fruit bunch using laccase and cutinase. In: BioResources. 2019 ; Vol. 14, No. 4. pp. 8879-8891.
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