Environment contaminant of Bacillus cereus isolated from ready to eat meat curry collected at various locations in Malaysia

A. E. Ali, M. J. Msarah, Sahilah Abd. Mutalib

Research output: Contribution to journalArticle

Abstract

The aim of the study was to isolate and identify Bacillus cereus from meat curry and to subtype the isolated B. cereus using RAPD-PCR and antibiotic resistance pattern. Ready to eat (RTE) meat curry samples purchased from 12 different restaurants at Kajang, Serdang and KL Sentral regions located in Selangor and Kuala Lumpur, Malaysia. Twenty-four isolates biochemically identified as B. cereus. Antimicrobial resistance analysis demonstrated that B. cereus isolates were highly resistance to ceftriaxone (100%), vancomycin (87.5%), clindamycin (91.6%) and nalidixic acid (100%). None of the B. cereus isolates were resistance towards ciprofloxacin (100%), streptomycin (91.6%) and chloramphenicol (83.4%). The B. cereus isolates were examined for randomly amplified polymorphic DNA-polymerase chain reaction (RAPDPCR) using primer S30 (5'-GTGATCGCAG-3') and discriminated into nine profiles. The antimicrobial analysis showed seven patterns and phenotypically less heterogeneous when compared to RAPD-PCR. A total number of nineteen types of B. cereus have produced by a combination of phenotype and genotype methods. These results demonstrated that both typing method provides evidence of the presence of similarity and diversity of the B. cereus strains from RTE meat curry.

Original languageEnglish
Pages (from-to)2640-2644
Number of pages5
JournalInternational Food Research Journal
Volume24
Issue number6
Publication statusPublished - 1 Dec 2017

Fingerprint

curry
Bacillus cereus
Malaysia
Meat
pollution
meat
Random Amplified Polymorphic DNA Technique
antibiotic resistance
ceftriaxone
clindamycin
Restaurants
Nalidixic Acid
vancomycin
nalidixic acid
Clindamycin
Ceftriaxone
restaurants
ciprofloxacin
DNA-directed DNA polymerase
Streptomycin

Keywords

  • Antimicrobial resistance
  • Biochemical test
  • RAPD-PCR

ASJC Scopus subject areas

  • Food Science

Cite this

Environment contaminant of Bacillus cereus isolated from ready to eat meat curry collected at various locations in Malaysia. / Ali, A. E.; Msarah, M. J.; Abd. Mutalib, Sahilah.

In: International Food Research Journal, Vol. 24, No. 6, 01.12.2017, p. 2640-2644.

Research output: Contribution to journalArticle

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