Enrichment, performance, and microbial diversity of a thermophilic mediatorless microbial fuel cell

Bor Chyan Jong, Byung Hong Kim, In Seop Chang, Pauline Woan Ying Liew, Yeng Fung Choo, Gi Su Kang

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

A thermophilic mediatorless microbial fuel cell (ML-MFC) was developed for continuous electricity production while treating artificial wastewater concurrently. A maximum power density of 1030 ± 340 mW/m2 was generated continuously at 55 °C with an anode retention time of 27 min (11 mL h-1) and continuous pumping of air-saturated phosphate buffer into the cathode compartment at the retention time of 0.7 min (450 mL h -1). Meanwhile, about 80% of the electrons available from acetate oxidation were recovered as current. Denaturing gradient gel electrophoresis (DGGE) and direct 16S-rRNA gene analysis revealed that the bacterial diversity in this ML-MFC system was lower than the inoculum. Direct 16S rDNA analysis showed that the dominant bacteria representing 57.8% of total population in anode compartment was phylogenetically very closely related to an uncultured clone, clone E4. Two sheets of graphite used as the anode showed different dominant bacterial population. For the first time, it is shown that thermophilic electrochemically active bacteria can be enriched to concurrently generate electricity and treat artificial wastewater in a thermophilic ML-MFC.

Original languageEnglish
Pages (from-to)6449-6454
Number of pages6
JournalEnvironmental Science and Technology
Volume40
Issue number20
DOIs
Publication statusPublished - 15 Oct 2006
Externally publishedYes

Fingerprint

Microbial fuel cells
fuel cell
Anodes
clone
Bacteria
electricity
Wastewater
Electricity
wastewater
bacterium
Graphite
Ribosomal DNA
Electrophoresis
graphite
pumping
electrokinesis
acetate
Buffers
Phosphates
Acetates

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Jong, B. C., Kim, B. H., Chang, I. S., Liew, P. W. Y., Choo, Y. F., & Kang, G. S. (2006). Enrichment, performance, and microbial diversity of a thermophilic mediatorless microbial fuel cell. Environmental Science and Technology, 40(20), 6449-6454. https://doi.org/10.1021/es0613512

Enrichment, performance, and microbial diversity of a thermophilic mediatorless microbial fuel cell. / Jong, Bor Chyan; Kim, Byung Hong; Chang, In Seop; Liew, Pauline Woan Ying; Choo, Yeng Fung; Kang, Gi Su.

In: Environmental Science and Technology, Vol. 40, No. 20, 15.10.2006, p. 6449-6454.

Research output: Contribution to journalArticle

Jong, Bor Chyan ; Kim, Byung Hong ; Chang, In Seop ; Liew, Pauline Woan Ying ; Choo, Yeng Fung ; Kang, Gi Su. / Enrichment, performance, and microbial diversity of a thermophilic mediatorless microbial fuel cell. In: Environmental Science and Technology. 2006 ; Vol. 40, No. 20. pp. 6449-6454.
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