Abstract
A thermophilic mediatorless microbial fuel cell (ML-MFC) was developed for continuous electricity production while treating artificial wastewater concurrently. A maximum power density of 1030 ± 340 mW/m2 was generated continuously at 55 °C with an anode retention time of 27 min (11 mL h-1) and continuous pumping of air-saturated phosphate buffer into the cathode compartment at the retention time of 0.7 min (450 mL h -1). Meanwhile, about 80% of the electrons available from acetate oxidation were recovered as current. Denaturing gradient gel electrophoresis (DGGE) and direct 16S-rRNA gene analysis revealed that the bacterial diversity in this ML-MFC system was lower than the inoculum. Direct 16S rDNA analysis showed that the dominant bacteria representing 57.8% of total population in anode compartment was phylogenetically very closely related to an uncultured clone, clone E4. Two sheets of graphite used as the anode showed different dominant bacterial population. For the first time, it is shown that thermophilic electrochemically active bacteria can be enriched to concurrently generate electricity and treat artificial wastewater in a thermophilic ML-MFC.
| Original language | English |
|---|---|
| Pages (from-to) | 6449-6454 |
| Number of pages | 6 |
| Journal | Environmental Science and Technology |
| Volume | 40 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 15 Oct 2006 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Environmental Engineering
- Environmental Science(all)
- Environmental Chemistry
Cite this
Enrichment, performance, and microbial diversity of a thermophilic mediatorless microbial fuel cell. / Jong, Bor Chyan; Kim, Byung Hong; Chang, In Seop; Liew, Pauline Woan Ying; Choo, Yeng Fung; Kang, Gi Su.
In: Environmental Science and Technology, Vol. 40, No. 20, 15.10.2006, p. 6449-6454.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enrichment, performance, and microbial diversity of a thermophilic mediatorless microbial fuel cell
AU - Jong, Bor Chyan
AU - Kim, Byung Hong
AU - Chang, In Seop
AU - Liew, Pauline Woan Ying
AU - Choo, Yeng Fung
AU - Kang, Gi Su
PY - 2006/10/15
Y1 - 2006/10/15
N2 - A thermophilic mediatorless microbial fuel cell (ML-MFC) was developed for continuous electricity production while treating artificial wastewater concurrently. A maximum power density of 1030 ± 340 mW/m2 was generated continuously at 55 °C with an anode retention time of 27 min (11 mL h-1) and continuous pumping of air-saturated phosphate buffer into the cathode compartment at the retention time of 0.7 min (450 mL h -1). Meanwhile, about 80% of the electrons available from acetate oxidation were recovered as current. Denaturing gradient gel electrophoresis (DGGE) and direct 16S-rRNA gene analysis revealed that the bacterial diversity in this ML-MFC system was lower than the inoculum. Direct 16S rDNA analysis showed that the dominant bacteria representing 57.8% of total population in anode compartment was phylogenetically very closely related to an uncultured clone, clone E4. Two sheets of graphite used as the anode showed different dominant bacterial population. For the first time, it is shown that thermophilic electrochemically active bacteria can be enriched to concurrently generate electricity and treat artificial wastewater in a thermophilic ML-MFC.
AB - A thermophilic mediatorless microbial fuel cell (ML-MFC) was developed for continuous electricity production while treating artificial wastewater concurrently. A maximum power density of 1030 ± 340 mW/m2 was generated continuously at 55 °C with an anode retention time of 27 min (11 mL h-1) and continuous pumping of air-saturated phosphate buffer into the cathode compartment at the retention time of 0.7 min (450 mL h -1). Meanwhile, about 80% of the electrons available from acetate oxidation were recovered as current. Denaturing gradient gel electrophoresis (DGGE) and direct 16S-rRNA gene analysis revealed that the bacterial diversity in this ML-MFC system was lower than the inoculum. Direct 16S rDNA analysis showed that the dominant bacteria representing 57.8% of total population in anode compartment was phylogenetically very closely related to an uncultured clone, clone E4. Two sheets of graphite used as the anode showed different dominant bacterial population. For the first time, it is shown that thermophilic electrochemically active bacteria can be enriched to concurrently generate electricity and treat artificial wastewater in a thermophilic ML-MFC.
UR - http://www.scopus.com/inward/record.url?scp=33750368884&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750368884&partnerID=8YFLogxK
U2 - 10.1021/es0613512
DO - 10.1021/es0613512
M3 - Article
C2 - 17120579
AN - SCOPUS:33750368884
VL - 40
SP - 6449
EP - 6454
JO - Environmental Science & Technology
JF - Environmental Science & Technology
SN - 0013-936X
IS - 20
ER -