Abstract
Our studies focused on improving the biocompatibility properties of two microfluidic prototyping substrates i.e. polyurethane methacrylate (PUMA) and off-stoichiometry thiol-ene (OSTE-80) polymer by Ar and N2 plasma treatment. The contact angle (CA) measurement showed that both plasma treatments inserted oxygen and nitrogen moieties increased the surface energy and hydrophilicity of PUMA and OSTE-80 polymer which corresponded to an increase of nitrogen to carbon ratios (N/C), as measured by XPS, to provide a conducive environment for cell attachments and proliferation. Under the SEM observation, the surface topography of PUMA and OSTE-80 polymer showed minimal changes after the plasma treatments. Furthermore, ageing studies showed that plasma-treated PUMA and OSTE-80 polymer had stable hydrophilicity and nitrogen composition during storage in ambient air for 15 days. After in vitro cell culture of human umbilical vein endothelial cells (HUVECs) on these surfaces for 24 h and 72 h, both trypan blue and alamar blue assays indicated that PUMA and OSTE-80 polymer treated with N2 plasma had the highest viability and proliferation. The polar nitrogen moieties, specifically amide groups, encouraged the HUVECs adhesion on the plasma-treated PUMA and OSTE-80 surfaces. Interestingly, PUMA polymer treated with Ar and N2 plasma showed different HUVECs morphology which was spindle and cobblestone-shaped respectively after 72 h of incubation. On the contrary, a monolayer of well-spread HUVECs formed on the Ar and N2 plasma-treated OSTE-80 polymers. These variable morphologies observed can be ascribed to the adherence HUVECs on the different elastic moduli of these surfaces whereby further investigation might be needed. Overall, Ar and N2 plasma treatment had successfully altered the surface properties of PUMA and OSTE-80 polymer by increasing its surface energy, hydrophilicity and chemical functionalities to create a biocompatible surface for HUVECs adhesion and proliferation.
| Original language | English |
|---|---|
| Pages (from-to) | 613-621 |
| Number of pages | 9 |
| Journal | Materials Science and Engineering C |
| Volume | 79 |
| DOIs | |
| Publication status | Published - 1 Oct 2017 |
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Keywords
- Biocompatibility
- Cell proliferation
- Hydrophilicity
- Microfluidics
- Plasma treatment
- Surface ageing
ASJC Scopus subject areas
- Materials Science(all)
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering
Cite this
Enhancing the biocompatibility of the polyurethane methacrylate and off-stoichiometry thiol-ene polymers by argon and nitrogen plasma treatment. / Chen, Tiam Foo; Siow, Kim Shyong; Ng, Pei Yuen; Yeop Majlis, Burhanuddin.
In: Materials Science and Engineering C, Vol. 79, 01.10.2017, p. 613-621.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancing the biocompatibility of the polyurethane methacrylate and off-stoichiometry thiol-ene polymers by argon and nitrogen plasma treatment
AU - Chen, Tiam Foo
AU - Siow, Kim Shyong
AU - Ng, Pei Yuen
AU - Yeop Majlis, Burhanuddin
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Our studies focused on improving the biocompatibility properties of two microfluidic prototyping substrates i.e. polyurethane methacrylate (PUMA) and off-stoichiometry thiol-ene (OSTE-80) polymer by Ar and N2 plasma treatment. The contact angle (CA) measurement showed that both plasma treatments inserted oxygen and nitrogen moieties increased the surface energy and hydrophilicity of PUMA and OSTE-80 polymer which corresponded to an increase of nitrogen to carbon ratios (N/C), as measured by XPS, to provide a conducive environment for cell attachments and proliferation. Under the SEM observation, the surface topography of PUMA and OSTE-80 polymer showed minimal changes after the plasma treatments. Furthermore, ageing studies showed that plasma-treated PUMA and OSTE-80 polymer had stable hydrophilicity and nitrogen composition during storage in ambient air for 15 days. After in vitro cell culture of human umbilical vein endothelial cells (HUVECs) on these surfaces for 24 h and 72 h, both trypan blue and alamar blue assays indicated that PUMA and OSTE-80 polymer treated with N2 plasma had the highest viability and proliferation. The polar nitrogen moieties, specifically amide groups, encouraged the HUVECs adhesion on the plasma-treated PUMA and OSTE-80 surfaces. Interestingly, PUMA polymer treated with Ar and N2 plasma showed different HUVECs morphology which was spindle and cobblestone-shaped respectively after 72 h of incubation. On the contrary, a monolayer of well-spread HUVECs formed on the Ar and N2 plasma-treated OSTE-80 polymers. These variable morphologies observed can be ascribed to the adherence HUVECs on the different elastic moduli of these surfaces whereby further investigation might be needed. Overall, Ar and N2 plasma treatment had successfully altered the surface properties of PUMA and OSTE-80 polymer by increasing its surface energy, hydrophilicity and chemical functionalities to create a biocompatible surface for HUVECs adhesion and proliferation.
AB - Our studies focused on improving the biocompatibility properties of two microfluidic prototyping substrates i.e. polyurethane methacrylate (PUMA) and off-stoichiometry thiol-ene (OSTE-80) polymer by Ar and N2 plasma treatment. The contact angle (CA) measurement showed that both plasma treatments inserted oxygen and nitrogen moieties increased the surface energy and hydrophilicity of PUMA and OSTE-80 polymer which corresponded to an increase of nitrogen to carbon ratios (N/C), as measured by XPS, to provide a conducive environment for cell attachments and proliferation. Under the SEM observation, the surface topography of PUMA and OSTE-80 polymer showed minimal changes after the plasma treatments. Furthermore, ageing studies showed that plasma-treated PUMA and OSTE-80 polymer had stable hydrophilicity and nitrogen composition during storage in ambient air for 15 days. After in vitro cell culture of human umbilical vein endothelial cells (HUVECs) on these surfaces for 24 h and 72 h, both trypan blue and alamar blue assays indicated that PUMA and OSTE-80 polymer treated with N2 plasma had the highest viability and proliferation. The polar nitrogen moieties, specifically amide groups, encouraged the HUVECs adhesion on the plasma-treated PUMA and OSTE-80 surfaces. Interestingly, PUMA polymer treated with Ar and N2 plasma showed different HUVECs morphology which was spindle and cobblestone-shaped respectively after 72 h of incubation. On the contrary, a monolayer of well-spread HUVECs formed on the Ar and N2 plasma-treated OSTE-80 polymers. These variable morphologies observed can be ascribed to the adherence HUVECs on the different elastic moduli of these surfaces whereby further investigation might be needed. Overall, Ar and N2 plasma treatment had successfully altered the surface properties of PUMA and OSTE-80 polymer by increasing its surface energy, hydrophilicity and chemical functionalities to create a biocompatible surface for HUVECs adhesion and proliferation.
KW - Biocompatibility
KW - Cell proliferation
KW - Hydrophilicity
KW - Microfluidics
KW - Plasma treatment
KW - Surface ageing
UR - http://www.scopus.com/inward/record.url?scp=85019545170&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019545170&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2017.05.091
DO - 10.1016/j.msec.2017.05.091
M3 - Article
VL - 79
SP - 613
EP - 621
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -