Enhancing the biocompatibility of the polyurethane methacrylate and off-stoichiometry thiol-ene polymers by argon and nitrogen plasma treatment

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Abstract

Our studies focused on improving the biocompatibility properties of two microfluidic prototyping substrates i.e. polyurethane methacrylate (PUMA) and off-stoichiometry thiol-ene (OSTE-80) polymer by Ar and N2 plasma treatment. The contact angle (CA) measurement showed that both plasma treatments inserted oxygen and nitrogen moieties increased the surface energy and hydrophilicity of PUMA and OSTE-80 polymer which corresponded to an increase of nitrogen to carbon ratios (N/C), as measured by XPS, to provide a conducive environment for cell attachments and proliferation. Under the SEM observation, the surface topography of PUMA and OSTE-80 polymer showed minimal changes after the plasma treatments. Furthermore, ageing studies showed that plasma-treated PUMA and OSTE-80 polymer had stable hydrophilicity and nitrogen composition during storage in ambient air for 15 days. After in vitro cell culture of human umbilical vein endothelial cells (HUVECs) on these surfaces for 24 h and 72 h, both trypan blue and alamar blue assays indicated that PUMA and OSTE-80 polymer treated with N2 plasma had the highest viability and proliferation. The polar nitrogen moieties, specifically amide groups, encouraged the HUVECs adhesion on the plasma-treated PUMA and OSTE-80 surfaces. Interestingly, PUMA polymer treated with Ar and N2 plasma showed different HUVECs morphology which was spindle and cobblestone-shaped respectively after 72 h of incubation. On the contrary, a monolayer of well-spread HUVECs formed on the Ar and N2 plasma-treated OSTE-80 polymers. These variable morphologies observed can be ascribed to the adherence HUVECs on the different elastic moduli of these surfaces whereby further investigation might be needed. Overall, Ar and N2 plasma treatment had successfully altered the surface properties of PUMA and OSTE-80 polymer by increasing its surface energy, hydrophilicity and chemical functionalities to create a biocompatible surface for HUVECs adhesion and proliferation.

Original languageEnglish
Pages (from-to)613-621
Number of pages9
JournalMaterials Science and Engineering C
Volume79
DOIs
Publication statusPublished - 1 Oct 2017

Fingerprint

Nitrogen plasma
Polyurethanes
nitrogen plasma
Methacrylates
Argon
argon plasma
biocompatibility
Biocompatibility
Sulfhydryl Compounds
thiols
Stoichiometry
stoichiometry
Polymers
Endothelial cells
Plasmas
veins
polymers
Hydrophilicity
Nitrogen
nitrogen

Keywords

  • Biocompatibility
  • Cell proliferation
  • Hydrophilicity
  • Microfluidics
  • Plasma treatment
  • Surface ageing

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

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title = "Enhancing the biocompatibility of the polyurethane methacrylate and off-stoichiometry thiol-ene polymers by argon and nitrogen plasma treatment",
abstract = "Our studies focused on improving the biocompatibility properties of two microfluidic prototyping substrates i.e. polyurethane methacrylate (PUMA) and off-stoichiometry thiol-ene (OSTE-80) polymer by Ar and N2 plasma treatment. The contact angle (CA) measurement showed that both plasma treatments inserted oxygen and nitrogen moieties increased the surface energy and hydrophilicity of PUMA and OSTE-80 polymer which corresponded to an increase of nitrogen to carbon ratios (N/C), as measured by XPS, to provide a conducive environment for cell attachments and proliferation. Under the SEM observation, the surface topography of PUMA and OSTE-80 polymer showed minimal changes after the plasma treatments. Furthermore, ageing studies showed that plasma-treated PUMA and OSTE-80 polymer had stable hydrophilicity and nitrogen composition during storage in ambient air for 15 days. After in vitro cell culture of human umbilical vein endothelial cells (HUVECs) on these surfaces for 24 h and 72 h, both trypan blue and alamar blue assays indicated that PUMA and OSTE-80 polymer treated with N2 plasma had the highest viability and proliferation. The polar nitrogen moieties, specifically amide groups, encouraged the HUVECs adhesion on the plasma-treated PUMA and OSTE-80 surfaces. Interestingly, PUMA polymer treated with Ar and N2 plasma showed different HUVECs morphology which was spindle and cobblestone-shaped respectively after 72 h of incubation. On the contrary, a monolayer of well-spread HUVECs formed on the Ar and N2 plasma-treated OSTE-80 polymers. These variable morphologies observed can be ascribed to the adherence HUVECs on the different elastic moduli of these surfaces whereby further investigation might be needed. Overall, Ar and N2 plasma treatment had successfully altered the surface properties of PUMA and OSTE-80 polymer by increasing its surface energy, hydrophilicity and chemical functionalities to create a biocompatible surface for HUVECs adhesion and proliferation.",
keywords = "Biocompatibility, Cell proliferation, Hydrophilicity, Microfluidics, Plasma treatment, Surface ageing",
author = "Chen, {Tiam Foo} and Siow, {Kim Shyong} and Ng, {Pei Yuen} and {Yeop Majlis}, Burhanuddin",
year = "2017",
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language = "English",
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T1 - Enhancing the biocompatibility of the polyurethane methacrylate and off-stoichiometry thiol-ene polymers by argon and nitrogen plasma treatment

AU - Chen, Tiam Foo

AU - Siow, Kim Shyong

AU - Ng, Pei Yuen

AU - Yeop Majlis, Burhanuddin

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Our studies focused on improving the biocompatibility properties of two microfluidic prototyping substrates i.e. polyurethane methacrylate (PUMA) and off-stoichiometry thiol-ene (OSTE-80) polymer by Ar and N2 plasma treatment. The contact angle (CA) measurement showed that both plasma treatments inserted oxygen and nitrogen moieties increased the surface energy and hydrophilicity of PUMA and OSTE-80 polymer which corresponded to an increase of nitrogen to carbon ratios (N/C), as measured by XPS, to provide a conducive environment for cell attachments and proliferation. Under the SEM observation, the surface topography of PUMA and OSTE-80 polymer showed minimal changes after the plasma treatments. Furthermore, ageing studies showed that plasma-treated PUMA and OSTE-80 polymer had stable hydrophilicity and nitrogen composition during storage in ambient air for 15 days. After in vitro cell culture of human umbilical vein endothelial cells (HUVECs) on these surfaces for 24 h and 72 h, both trypan blue and alamar blue assays indicated that PUMA and OSTE-80 polymer treated with N2 plasma had the highest viability and proliferation. The polar nitrogen moieties, specifically amide groups, encouraged the HUVECs adhesion on the plasma-treated PUMA and OSTE-80 surfaces. Interestingly, PUMA polymer treated with Ar and N2 plasma showed different HUVECs morphology which was spindle and cobblestone-shaped respectively after 72 h of incubation. On the contrary, a monolayer of well-spread HUVECs formed on the Ar and N2 plasma-treated OSTE-80 polymers. These variable morphologies observed can be ascribed to the adherence HUVECs on the different elastic moduli of these surfaces whereby further investigation might be needed. Overall, Ar and N2 plasma treatment had successfully altered the surface properties of PUMA and OSTE-80 polymer by increasing its surface energy, hydrophilicity and chemical functionalities to create a biocompatible surface for HUVECs adhesion and proliferation.

AB - Our studies focused on improving the biocompatibility properties of two microfluidic prototyping substrates i.e. polyurethane methacrylate (PUMA) and off-stoichiometry thiol-ene (OSTE-80) polymer by Ar and N2 plasma treatment. The contact angle (CA) measurement showed that both plasma treatments inserted oxygen and nitrogen moieties increased the surface energy and hydrophilicity of PUMA and OSTE-80 polymer which corresponded to an increase of nitrogen to carbon ratios (N/C), as measured by XPS, to provide a conducive environment for cell attachments and proliferation. Under the SEM observation, the surface topography of PUMA and OSTE-80 polymer showed minimal changes after the plasma treatments. Furthermore, ageing studies showed that plasma-treated PUMA and OSTE-80 polymer had stable hydrophilicity and nitrogen composition during storage in ambient air for 15 days. After in vitro cell culture of human umbilical vein endothelial cells (HUVECs) on these surfaces for 24 h and 72 h, both trypan blue and alamar blue assays indicated that PUMA and OSTE-80 polymer treated with N2 plasma had the highest viability and proliferation. The polar nitrogen moieties, specifically amide groups, encouraged the HUVECs adhesion on the plasma-treated PUMA and OSTE-80 surfaces. Interestingly, PUMA polymer treated with Ar and N2 plasma showed different HUVECs morphology which was spindle and cobblestone-shaped respectively after 72 h of incubation. On the contrary, a monolayer of well-spread HUVECs formed on the Ar and N2 plasma-treated OSTE-80 polymers. These variable morphologies observed can be ascribed to the adherence HUVECs on the different elastic moduli of these surfaces whereby further investigation might be needed. Overall, Ar and N2 plasma treatment had successfully altered the surface properties of PUMA and OSTE-80 polymer by increasing its surface energy, hydrophilicity and chemical functionalities to create a biocompatible surface for HUVECs adhesion and proliferation.

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KW - Hydrophilicity

KW - Microfluidics

KW - Plasma treatment

KW - Surface ageing

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