Peningkatan pengekstrakan minyak kelapa sawit melalui penggunaan enzim degradasi dinding sel

Translated title of the contribution: Enhancement of palm oil extraction using cell wall degrading enzyme formulation

Hemavathi Silvamany, Jamaliah Md Jahim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this recent work, application of aqueous enzymatic process to enhance recovery of palm oil was studied. Experiments were carried out to investigate the structural carbohydrate composition of oil palm mesocarp (Elaeis guineensis) and to analyze the effect of different combination of enzymes on the palm oil recovery and degree of digestibility and the respective correlation. The optimum combination of enzymes comprising of Cellic CTec2 (X1), Cellic HTec2 (X2) and Pectinex Ultra SP-L (X3) for Aqueous Enzymatic Oil Extraction Process (AEOEP), were determined using Simplex Lattice mixture design under fixed parameters. Maximum oil recovery of 88% was achieved with ratio of enzymes at 0.46: 0.34: 0.2 (X1:X2:X3), at enzyme loading of 30 mgprotein /10 gsubstrate, substrate loading of 50%w/v, pH 4.8, and 2 hours of incubation at 50 ºC. The conversion of reducing sugar at corresponding condition was measured to evaluate the effectiveness of enzymes in degrading fruit cell wall releasing trapped oil. Moreover, transmission electron microscopy (TEM) was utilized to indicate the increase in cell wall disintegration leading to higher release of oil with enzymatic treatment.

Original languageUndefined/Unknown
Pages (from-to)77-87
Number of pages11
JournalMalaysian Journal of Analytical Sciences
Volume19
Issue number1
Publication statusPublished - 2015

Fingerprint

Cells
Oils
Enzymes
Recovery
Palm oil
Disintegration
Fruits
Sugars
Carbohydrates
palm oil
Transmission electron microscopy
Substrates
Chemical analysis
Experiments

Keywords

  • Aqueous enzymatic process
  • Cellic CTec2
  • Cellic HTec2
  • Elaeis guineensis
  • Palm oil extraction
  • Pectinex Ultra SPL

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Peningkatan pengekstrakan minyak kelapa sawit melalui penggunaan enzim degradasi dinding sel. / Silvamany, Hemavathi; Md Jahim, Jamaliah.

In: Malaysian Journal of Analytical Sciences, Vol. 19, No. 1, 2015, p. 77-87.

Research output: Contribution to journalArticle

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