Enhancement of docosahexaenoic acid production by Schizochytrium SW1 using response surface methodology

Mohd Yusuf Mohd Nazir, Najeeb Kaid Nasser Al-Shorgani, Mohd. Sahaid Kalil, Aidil Abdul Hamid

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, three factors (fructose concentration, agitation speed and monosodium glutamate (MSG) concentration) were optimized to enhance DHA production by Schizochytrium SW1 using response surface methodology (RSM). Central composite design was applied as the experimental design and analysis of variance (ANOVA) was used to analyze the data. The experiments were conducted using 500 mL flask with 100 mL working volume at 30°C for 96 hours. ANOVA analysis revealed that the process was adequately represented significantly by the quadratic model (p<0.0001) and two of the factors namely agitation speed and MSG concentration significantly affect DHA production (p<0.005). Level of influence for each variable and quadratic polynomial equation were obtained for DHA production by multiple regression analyses. The estimated optimum conditions for maximizing DHA production by SW1 were 70 g/L fructose, 250 rpm agitation speed and 12 g/L MSG. Consequently, the quadratic model was validated by applying of the estimated optimum conditions, which confirmed the model validity and 52.86% of DHA was produced.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

glutamates
agitation
methodology
analysis of variance
acids
augmentation
flasks
regression analysis
polynomials
composite materials

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Nazir, M. Y. M., Al-Shorgani, N. K. N., Kalil, M. S., & Abdul Hamid, A. (2015). Enhancement of docosahexaenoic acid production by Schizochytrium SW1 using response surface methodology. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [030009] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931230

Enhancement of docosahexaenoic acid production by Schizochytrium SW1 using response surface methodology. / Nazir, Mohd Yusuf Mohd; Al-Shorgani, Najeeb Kaid Nasser; Kalil, Mohd. Sahaid; Abdul Hamid, Aidil.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 030009.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nazir, MYM, Al-Shorgani, NKN, Kalil, MS & Abdul Hamid, A 2015, Enhancement of docosahexaenoic acid production by Schizochytrium SW1 using response surface methodology. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 030009, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931230
Nazir MYM, Al-Shorgani NKN, Kalil MS, Abdul Hamid A. Enhancement of docosahexaenoic acid production by Schizochytrium SW1 using response surface methodology. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 030009 https://doi.org/10.1063/1.4931230
Nazir, Mohd Yusuf Mohd ; Al-Shorgani, Najeeb Kaid Nasser ; Kalil, Mohd. Sahaid ; Abdul Hamid, Aidil. / Enhancement of docosahexaenoic acid production by Schizochytrium SW1 using response surface methodology. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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