Enhancement of Biobutanol production by butyric acid addition using Clostridium saccharoperbutytacetonicum N1-4 (ATCC 13564)

N. K N Al-Shorgani, Mohd. Sahaid Kalil, E. Ali, W. M W Yusofl, Aidil Abdul Hamid

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Butanol is an important industrial chemical and has gained attention as an important fuel because of its advantages of being less corrosive and water tolerant as compared to the ethanol. This study revealed the effects of butyric acid as an additive on growth and Acetone-butanol-ethanol (ABE) production using batch culture of Clostridium saccharoperbutylacetonicum Ni-4. Different combinations of glucose and butyric acid were studied to finalize the best productive ratio for ABE and butanol production. The highest ABE and butanol production was obtained when 4 g L-1of butyric acid was used in the presence of 30 g L of glucose. The inhibitory effects of butyric acid on bacterial growth were also investigated using C. saccharoperbutylacetonicum Ni-4 and mild inhibitory effects were found at high butyric acid concentration. On the other hand, no linear correlation between butyric acid and butanol production was observed. Production ofl7.76gL -1butanolwithaproducthityofoi5gL h 'from 4g L ' of butyric acid proedthe ability of C. saccharoperbutylacetonicum N1-4 to be tolerant to the certain concentration of butyric acid for the enhanced butanol production Butyric acid was not only contributing as an additive or stimulating agent to the butanol pathway but also was being utilized as a co-substrate. Enhanced butanol production using growing cells of C. saceharoperbutylacetonicum NL4 in the presence of specific concentration of butyric acid (4 g L -1butyric acid) as a co-substrate with glucose can be carried out without any remarkable inhibition to bacterial growth.

Original languageEnglish
Pages (from-to)326-332
Number of pages7
JournalBiotechnology
Volume11
Issue number6
DOIs
Publication statusPublished - 2012

Fingerprint

Clostridium
Butanols
Butyric Acid
Ethanol
Acetone
Glucose
Growth
Industrial chemicals
Caustics
Batch Cell Culture Techniques
Substrates

Keywords

  • Batch culture
  • Biobutanol fermentation
  • Butyric acid
  • Clostridium saccizaroperbulylacetonicum N-4

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Enhancement of Biobutanol production by butyric acid addition using Clostridium saccharoperbutytacetonicum N1-4 (ATCC 13564). / Al-Shorgani, N. K N; Kalil, Mohd. Sahaid; Ali, E.; Yusofl, W. M W; Abdul Hamid, Aidil.

In: Biotechnology, Vol. 11, No. 6, 2012, p. 326-332.

Research output: Contribution to journalArticle

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AU - Abdul Hamid, Aidil

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AB - Butanol is an important industrial chemical and has gained attention as an important fuel because of its advantages of being less corrosive and water tolerant as compared to the ethanol. This study revealed the effects of butyric acid as an additive on growth and Acetone-butanol-ethanol (ABE) production using batch culture of Clostridium saccharoperbutylacetonicum Ni-4. Different combinations of glucose and butyric acid were studied to finalize the best productive ratio for ABE and butanol production. The highest ABE and butanol production was obtained when 4 g L-1of butyric acid was used in the presence of 30 g L of glucose. The inhibitory effects of butyric acid on bacterial growth were also investigated using C. saccharoperbutylacetonicum Ni-4 and mild inhibitory effects were found at high butyric acid concentration. On the other hand, no linear correlation between butyric acid and butanol production was observed. Production ofl7.76gL -1butanolwithaproducthityofoi5gL h 'from 4g L ' of butyric acid proedthe ability of C. saccharoperbutylacetonicum N1-4 to be tolerant to the certain concentration of butyric acid for the enhanced butanol production Butyric acid was not only contributing as an additive or stimulating agent to the butanol pathway but also was being utilized as a co-substrate. Enhanced butanol production using growing cells of C. saceharoperbutylacetonicum NL4 in the presence of specific concentration of butyric acid (4 g L -1butyric acid) as a co-substrate with glucose can be carried out without any remarkable inhibition to bacterial growth.

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