Enhanced topical delivery and ex vivo anti-inflammatory activity from a betamethasone dipropionate formulation containing fish oil

Mohd Hanif Zulfakar, Nassima Abdelouahab, Charles M. Heard

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objective and design: To probe ex vivo the influence of fish oil (FO) on the topical delivery and anti-inflammatory properties of betamethasone dipropionate (BD). Materials or subjects: Freshly excised porcine ear skin. Treatment: Ointment formulations containing BD + salicylic acid (SA), BD + SA + FO, or base as control, applied to the skin mounted in Franz cells. Methods: Comparative depth profiling; skin probed by immunohistochemistry for cyclooxygenase-2 (COX-2) and by ELISA for prostaglandin E2 (PGE2). Results: More BD was obtained in the first 30 layers and the remaining epidermis with BD + SA. However, more penetrants were recovered from the remaining skin treated with BD + SA + FO. Although BD + SA reduced COX-2 expression within the epidermis, greater reduction was observed with BD + SA + FO as indicated by reduced COX-2 expression. FO alone had a comparable effect on the expression of COX-2. Modulation of PGE2 production also supported the anti-inflammatory properties of fish oil, reducing PGE2 levels by an amount comparable to the reduction by BD. Combining FO and BD, however, did not provide the anticipated potentiation effect. Conclusions: Fish oil enhanced the delivery of BD and SA across skin. Addition of fish oil also enhanced the anti-inflammatory activity of BD, attributed to increased amounts of BD present in the skin and/or the intrinsic anti-inflammatory activity of fish oil.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalInflammation Research
Volume59
Issue number1
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

Fingerprint

Fish Oils
Anti-Inflammatory Agents
Cyclooxygenase 2
Skin
Dinoprostone
Epidermis
betamethasone-17,21-dipropionate
Ointments
salicylic acid drug combination betamethasone dipropionate
Ear
Swine
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

Keywords

  • COX
  • Fatty acids
  • Models
  • PGs
  • Skin inflammation

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

Enhanced topical delivery and ex vivo anti-inflammatory activity from a betamethasone dipropionate formulation containing fish oil. / Zulfakar, Mohd Hanif; Abdelouahab, Nassima; Heard, Charles M.

In: Inflammation Research, Vol. 59, No. 1, 01.2010, p. 23-30.

Research output: Contribution to journalArticle

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