Enhanced tolerance to salinity stress and ABA is regulated by Oryza sativa STRESS ASSOCIATED PROTEIN 8 (OsSAP8)

Nur Farhana Roslan, Nor Syafiqah Abd Rashid, Intan Elya Suka, Nadiatul Ain Nabila Ahmad Taufik, Nur Syahirah Abdullah, Mohd Bajuri Asruri, Bernadette Toni, Noor Liyana Sukiran, Zamri Zainal, Nurulhikma Md Isa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Increased tolerance to multiple abiotic stresses in plants is a key target for food security. OsSAP8 is a member of the stress-associated protein (SAP) gene family in rice and is characterized by the presence of two zinc finger domains, A20 and AN1. In this research, we aim to understand the regulatory mechanism of OsSAP8 in response to multiple stresses for rice survival. We used the Arabidopsis transformation system to understand the gene's function. Two Arabidopsis lines were used; OsSAP8 overexpression line and Arabidopsis atsap2 knockout loss-of-function mutation in comparison to wild type Columbia-0 (Col-0). The protein sequence of Arabidopsis atsap2 has been shown to be highly identical to the OsSAP8. An atsap2 knockout showed a sensitive phenotype to high concentration of NaCl and PEG with reduced germination percentage. OsSAP8 overexpression showed a tolerance phenotype to 150 mM NaCl and 20% PEG compared to atsap2. OsSAP8 also showed an insensitive germination phenotype to high concentration of ABA compared to Col-0 and atsap2. The insensitive phenotype of OsSAP8 on the high concentration of ABA (12 μM) and the sensitive phenotype of its orthologue atsap2 in Arabidopsis suggests that OsSAP8 may have a role in part through ABA signaling as a putative transcriptional regulator for ABA during stress response.

Original languageEnglish
Pages (from-to)853-860
Number of pages8
JournalAustralian Journal of Crop Science
Volume11
Issue number7
DOIs
Publication statusPublished - 2017

Fingerprint

salt stress
Oryza sativa
Arabidopsis
phenotype
germination
rice
zinc finger motif
food security
abiotic stress
stress response
genes
amino acid sequences
transcription factors
proteins

Keywords

  • Abiotic stress
  • Arabidopsis
  • Atsap2 gene
  • Oryza sativa
  • OsSAP8 gene
  • Seed germination

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Enhanced tolerance to salinity stress and ABA is regulated by Oryza sativa STRESS ASSOCIATED PROTEIN 8 (OsSAP8). / Roslan, Nur Farhana; Rashid, Nor Syafiqah Abd; Suka, Intan Elya; Taufik, Nadiatul Ain Nabila Ahmad; Abdullah, Nur Syahirah; Asruri, Mohd Bajuri; Toni, Bernadette; Sukiran, Noor Liyana; Zainal, Zamri; Md Isa, Nurulhikma.

In: Australian Journal of Crop Science, Vol. 11, No. 7, 2017, p. 853-860.

Research output: Contribution to journalArticle

Roslan, Nur Farhana ; Rashid, Nor Syafiqah Abd ; Suka, Intan Elya ; Taufik, Nadiatul Ain Nabila Ahmad ; Abdullah, Nur Syahirah ; Asruri, Mohd Bajuri ; Toni, Bernadette ; Sukiran, Noor Liyana ; Zainal, Zamri ; Md Isa, Nurulhikma. / Enhanced tolerance to salinity stress and ABA is regulated by Oryza sativa STRESS ASSOCIATED PROTEIN 8 (OsSAP8). In: Australian Journal of Crop Science. 2017 ; Vol. 11, No. 7. pp. 853-860.
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