Enhanced sugar recovery from oil palm trunk biomass by repeated enzymatic hydrolysis with surfactant addition

Nurul Adela Bukhari, Soh Kheang Loh, Nasrin Abu Bakar, Jamaliah Md Jahim

Research output: Contribution to journalArticle

Abstract

In bioconversion, low sugar recovery is often faced during enzymatic hydrolysis process of agro-industrial biomass due to their complex crystalline structures. However, high enzyme loadings make large-scale processing economically unfeasible. Addition of surfactants to enzymatic hydrolysis has been found to enhance the conversion of cellulose to sugar. In this study, we have attempted to enhance sugar recovery from oil palm trunk biomass (OPTB) by investigating the co-existence of surfactants in the enzyme employed. The study revealed that addition of non-ionic surfactant i.e. Triton X-100 at 0.1% (v/v) facilitated hydrolysis and improved the sugar recovery from the pretreated OPTB by 2-fold within 48 hr. In addition, a repeated enzymatic hydrolysis (REH) was employed where the hydrolysate from the initial process was recycled and reused for subsequent cycle. From the two-cycle of REH, a total recovered sugar of 36.9±0.8 g/L (72.6% yield) was obtained within 96 hr, while the three-cycle REH recovered 55.1±1.7 g/L total sugar (72.2% yield) within a shorter reaction time of 72 hr. Recycling and reusing the hydrolysate combined with supplementing surfactant has reduced the enzyme usage by half, thereby resulted in substantial savings for improved process feasibility.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalMalaysian Applied Biology
Volume47
Issue number5
Publication statusPublished - 1 Nov 2018

Fingerprint

Elaeis guineensis
enzymatic hydrolysis
Surface-Active Agents
surfactants
Biomass
Oils
Hydrolysis
sugars
biomass
hydrolysates
Enzymes
enzymes
nonionic surfactants
Octoxynol
Recycling
crystal structure
biotransformation
Cellulose
recycling
cellulose

Keywords

  • Enzymatic saccharification
  • Fermentable sugar
  • Lignocellulosic biomass
  • Oil palm trunk
  • Triton X-100

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Enhanced sugar recovery from oil palm trunk biomass by repeated enzymatic hydrolysis with surfactant addition. / Bukhari, Nurul Adela; Loh, Soh Kheang; Bakar, Nasrin Abu; Md Jahim, Jamaliah.

In: Malaysian Applied Biology, Vol. 47, No. 5, 01.11.2018, p. 165-172.

Research output: Contribution to journalArticle

Bukhari, Nurul Adela ; Loh, Soh Kheang ; Bakar, Nasrin Abu ; Md Jahim, Jamaliah. / Enhanced sugar recovery from oil palm trunk biomass by repeated enzymatic hydrolysis with surfactant addition. In: Malaysian Applied Biology. 2018 ; Vol. 47, No. 5. pp. 165-172.
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