Enhanced in vitro angiogenic behaviour of human umbilical vein endothelial cells on thermally oxidized TiO 2 nanofibrous surfaces

Ai Wen Tan, Ling Ling Liau, Chua Kien Hui, Roslina Ahmad, Sheikh Ali Akbar, Belinda Pingguan-Murphy

Research output: Contribution to journalArticle

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Abstract

One of the major challenges in bone grafting is the lack of sufficient bone vascularization. A rapid and stable bone vascularization at an early stage of implantation is essential for optimal functioning of the bone graft. To address this, the ability of in situ TiO 2 nanofibrous surfaces fabricated via thermal oxidation method to enhance the angiogenic potential of human umbilical vein endothelial cells (HUVECs) was investigated. The cellular responses of HUVECs on TiO 2 nanofibrous surfaces were studied through cell adhesion, cell proliferation, capillary-like tube formation, growth factors secretion (VEGF and BFGF), and angiogenic-endogenic-associated gene (VEGF, VEGFR2, BFGF, PGF, HGF, Ang-1, VWF, PECAM-1 and ENOS) expression analysis after 2 weeks of cell seeding. Our results show that TiO 2 nanofibrous surfaces significantly enhanced adhesion, proliferation, formation of capillary-like tube networks and growth factors secretion of HUVECs, as well as leading to higher expression level of all angiogenic-endogenic-associated genes, in comparison to unmodified control surfaces. These beneficial effects suggest the potential use of such surface nanostructures to be utilized as an advantageous interface for bone grafts as they can promote angiogenesis, which improves bone vascularization.

Original languageEnglish
Article number21828
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 17 Feb 2016

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Human Umbilical Vein Endothelial Cells
Bone and Bones
Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
CD31 Antigens
Transplants
Bone Transplantation
Nanostructures
Prostaglandins F
Cell Adhesion
Genes
Hot Temperature
Cell Proliferation
In Vitro Techniques

ASJC Scopus subject areas

  • General

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Enhanced in vitro angiogenic behaviour of human umbilical vein endothelial cells on thermally oxidized TiO 2 nanofibrous surfaces. / Tan, Ai Wen; Liau, Ling Ling; Kien Hui, Chua; Ahmad, Roslina; Akbar, Sheikh Ali; Pingguan-Murphy, Belinda.

In: Scientific Reports, Vol. 6, 21828, 17.02.2016.

Research output: Contribution to journalArticle

Tan, Ai Wen ; Liau, Ling Ling ; Kien Hui, Chua ; Ahmad, Roslina ; Akbar, Sheikh Ali ; Pingguan-Murphy, Belinda. / Enhanced in vitro angiogenic behaviour of human umbilical vein endothelial cells on thermally oxidized TiO 2 nanofibrous surfaces. In: Scientific Reports. 2016 ; Vol. 6.
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