Enhanced butanol production by Clostridium acetobutylicum NCIMB 13357 grown on date fruit as carbon source in P2 medium

Emran I. Khamaiseh, Aidil Abdul Hamid, Peyman Abdeshahian, Wan Mohtar Wan Yusoff, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The production of biobutanol was studied by the cultivation of Clostridium acetobutylicum NCIMB 13557 in P2 medium including date fruit as the sole substrate. The effect of P2 medium and the effect of different concentrations of date fruit ranging from 10 to 100 g/L on biobutanol production were investigated. Anaerobic batch culture was carried out at 35°C incubation temperature and pH 7.0 ± 0.2 for 72 h. Experimental results showed that the lowest yield of biobutanol and acetone-butanol-ethanol (ABE) was 0.32 and 0.35 gram per gram of carbohydrate consumed (g/g), respectively, when an initial date fruit concentration of 10 g/L was utilized. At this fruit date concentration a biobutanol production value of 1.56 g/L was obtained. On the other hand, the maximum yield of biobutanol (0.48 g/g) and ABE (0.63 g/g) was produced at 50 g/L date fruit concentration with a biobutanol production value as high as 11 g/L. However, when a higher initial date fruit concentration was used, biobutanol and ABE production decreased to reach the yield of 0.22 g/g and 0.35 g/g, respectively, where 100 g/L date fruit was used. Similar results also revealed that 10.03 g/L biobutanol was produced using 100 g/L date fruit.

Original languageEnglish
Article number395754
JournalThe Scientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014

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Clostridium acetobutylicum
Clostridium
Butanols
Fruits
Carbon
fruit
carbon
Acetone
acetone
ethanol
Ethanol
Batch Cell Culture Techniques
Phoeniceae
carbohydrate
incubation
Carbohydrates
substrate
Temperature

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Enhanced butanol production by Clostridium acetobutylicum NCIMB 13357 grown on date fruit as carbon source in P2 medium. / Khamaiseh, Emran I.; Abdul Hamid, Aidil; Abdeshahian, Peyman; Wan Yusoff, Wan Mohtar; Kalil, Mohd. Sahaid.

In: The Scientific World Journal, Vol. 2014, 395754, 2014.

Research output: Contribution to journalArticle

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