Enhanced biocatalytic esterification with lipase-immobilized chitosan/graphene oxide beads

Siaw Cheng Lau, Hong Ngee Lim, Mahiran Basri, Hamid Reza Fard Masoumi, Asilah Ahmad Tajudin, Nay Ming Huang, Alagarsamy Pandikumar, Chin Hua Chia, Yoshito Andou

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this work, lipase from Candida rugosa was immobilized onto chitosan/graphene oxide beads. This was to provide an enzyme-immobilizing carrier with excellent enzyme immobilization activity for an enzyme group requiring hydrophilicity on the immobilizing carrier. In addition, this work involved a process for the preparation of an enzymatically active product insoluble in a reaction medium consisting of lauric acid and oleyl alcohol as reactants and hexane as a solvent. This product enabled the stability of the enzyme under the working conditions and allowed the enzyme to be readily isolated from the support. In particular, this meant that an enzymatic reaction could be stopped by the simple mechanical separation of the " insoluble" enzyme from the reaction medium. Chitosan was incorporated with graphene oxide because the latter was able to enhance the physical strength of the chitosan beads by its superior mechanical integrity and low thermal conductivity. The X-ray diffraction pattern showed that the graphene oxide was successfully embedded within the structure of the chitosan. Further, the lipase incorporation on the beads was confirmed by a thermo-gravimetric analysis. The lipase immobilization on the beads involved the functionalization with coupling agents, N-hydroxysulfosuccinimide sodium (NHS) and 1-ethyl-(3- dimethylaminopropyl) carbodiimide (EDC), and it possessed a high enzyme activity of 64 U. The overall esterification conversion of the prepared product was 78% at 60°C, and it attained conversions of 98% and 88% with commercially available lipozyme and novozyme, respectively, under similar experimental conditions.

Original languageEnglish
Article numbere104695
JournalPLoS One
Volume9
Issue number8
DOIs
Publication statusPublished - 15 Aug 2014

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Esterification
Chitosan
esterification
chitosan
Lipase
Oxides
Enzymes
lauric acid
enzymes
enzyme activity
Candida rugosa
thermogravimetry
enzyme stability
Immobilization
immobilized enzymes
dodecanoic acid
thermal conductivity
Enzyme immobilization
enzymatic reactions

ASJC Scopus subject areas

  • Medicine(all)
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lau, S. C., Lim, H. N., Basri, M., Fard Masoumi, H. R., Ahmad Tajudin, A., Huang, N. M., ... Andou, Y. (2014). Enhanced biocatalytic esterification with lipase-immobilized chitosan/graphene oxide beads. PLoS One, 9(8), [e104695]. https://doi.org/10.1371/journal.pone.0104695

Enhanced biocatalytic esterification with lipase-immobilized chitosan/graphene oxide beads. / Lau, Siaw Cheng; Lim, Hong Ngee; Basri, Mahiran; Fard Masoumi, Hamid Reza; Ahmad Tajudin, Asilah; Huang, Nay Ming; Pandikumar, Alagarsamy; Chia, Chin Hua; Andou, Yoshito.

In: PLoS One, Vol. 9, No. 8, e104695, 15.08.2014.

Research output: Contribution to journalArticle

Lau, SC, Lim, HN, Basri, M, Fard Masoumi, HR, Ahmad Tajudin, A, Huang, NM, Pandikumar, A, Chia, CH & Andou, Y 2014, 'Enhanced biocatalytic esterification with lipase-immobilized chitosan/graphene oxide beads', PLoS One, vol. 9, no. 8, e104695. https://doi.org/10.1371/journal.pone.0104695
Lau SC, Lim HN, Basri M, Fard Masoumi HR, Ahmad Tajudin A, Huang NM et al. Enhanced biocatalytic esterification with lipase-immobilized chitosan/graphene oxide beads. PLoS One. 2014 Aug 15;9(8). e104695. https://doi.org/10.1371/journal.pone.0104695
Lau, Siaw Cheng ; Lim, Hong Ngee ; Basri, Mahiran ; Fard Masoumi, Hamid Reza ; Ahmad Tajudin, Asilah ; Huang, Nay Ming ; Pandikumar, Alagarsamy ; Chia, Chin Hua ; Andou, Yoshito. / Enhanced biocatalytic esterification with lipase-immobilized chitosan/graphene oxide beads. In: PLoS One. 2014 ; Vol. 9, No. 8.
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