Engineering the lactococcal mevalonate pathway for increased sesquiterpene production

Adelene A. Song, Janna Ong Abdullah, Mohd P. Abdullah, Norazizah Shafee, Roohaida Othman, Normah Mohd. Noor, Raha A. Rahim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Isoprenoids are a large, diverse group of secondary metabolites which has recently raised a renewed research interest due to genetic engineering advances, allowing specific isoprenoids to be produced and characterized in heterologous hosts. Many researches on metabolic engineering of heterologous hosts for increased isoprenoid production are focussed on Escherichia coli and yeasts. E. coli, as most prokaryotes, use the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway for isoprenoid production. Yeasts on the other hand, use the mevalonate pathway which is commonly found in eukaryotes. However, Lactococcus lactis is an attractive alternative host for heterologous isoprenoid production. Apart from being food-grade, this Gram-positive prokaryote uses the mevalonate pathway for isoprenoid production instead of the MEP pathway. Previous studies have shown that L. lactis is able to produce sesquiterpenes through heterologous expression of plant sesquiterpene synthases. In this work, we analysed the gene expression of the lactococcal mevalonate pathway through RT-qPCR to successfully engineer L. lactis as an efficient host for isoprenoid production. We then overexpressed the mvk gene singly or co-expressed with the mvaA gene as an attempt to increase β-sesquiphellandrene production in L. lactis. It was observed that co-expression of mvk with mvaA doubled the amount of β-sesquiphellandrene produced.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalFEMS Microbiology Letters
Volume355
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Mevalonic Acid
Sesquiterpenes
Terpenes
Yeasts
Escherichia coli
Metabolic Engineering
Lactococcus lactis
Genetic Engineering
Eukaryota
Research
Genes
Gene Expression
Food

Keywords

  • Isoprenoid
  • Lactococcus lactis
  • Metabolic engineering
  • Mevalonate pathway
  • RT-qPCR
  • Sesquiterpene

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Song, A. A., Ong Abdullah, J., Abdullah, M. P., Shafee, N., Othman, R., Mohd. Noor, N., & Rahim, R. A. (2014). Engineering the lactococcal mevalonate pathway for increased sesquiterpene production. FEMS Microbiology Letters, 355(2), 177-184. https://doi.org/10.1111/1574-6968.12469

Engineering the lactococcal mevalonate pathway for increased sesquiterpene production. / Song, Adelene A.; Ong Abdullah, Janna; Abdullah, Mohd P.; Shafee, Norazizah; Othman, Roohaida; Mohd. Noor, Normah; Rahim, Raha A.

In: FEMS Microbiology Letters, Vol. 355, No. 2, 2014, p. 177-184.

Research output: Contribution to journalArticle

Song, AA, Ong Abdullah, J, Abdullah, MP, Shafee, N, Othman, R, Mohd. Noor, N & Rahim, RA 2014, 'Engineering the lactococcal mevalonate pathway for increased sesquiterpene production', FEMS Microbiology Letters, vol. 355, no. 2, pp. 177-184. https://doi.org/10.1111/1574-6968.12469
Song, Adelene A. ; Ong Abdullah, Janna ; Abdullah, Mohd P. ; Shafee, Norazizah ; Othman, Roohaida ; Mohd. Noor, Normah ; Rahim, Raha A. / Engineering the lactococcal mevalonate pathway for increased sesquiterpene production. In: FEMS Microbiology Letters. 2014 ; Vol. 355, No. 2. pp. 177-184.
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