Endogenous and induced angiogenic characteristics of human chorion-derived stem cells

Mohd Manzor Nur Fariha, Chua Kien Hui, Geok Chin Tan, Yun Hsuen Lim, Abdul Rahman Hayati

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cell-based therapy using stem cells has emerged as one of the pro-angiogenic methods to enhance blood vessel growth and sprouting in ischaemic conditions. This study investigated the endogenous and induced angiogenic characteristics of hCDSC (human chorion-derived stem cell) using QPCR (quantitative PCR) method, immunocytochemistry and fibrin-matrigel migration assay. The results showed that cultured hCDSC endogenously expressed angiogenic-endogenicassociated genes (VEGF, bFGF, PGF, HGF, Ang-1, PECAM-1, eNOS, Ve-cad, CD34, VEGFR-2 and vWF), with significant increase in mRNA levels of PGF, HGF, Ang-1, eNOS, VEGFR-2 and vWF following induction by bFGF (basic fibroblastgrowth factor) and VEGF (vascular endothelial growth factor). These enhanced angiogenic properties suggest thatinduced hCDSC provides a stronger angiogenic effect for the treatment of ischaemia. After angiogenic induction, hCDSC showed no reduction in the expression of the stemness genes, but had significantly higher levels of mRNA of Oct-4, Nanog (3), FZD9, ABCG-2 and BST-1. The induced cells were positive for PECAM-1 (platelet/endothelial cell adhesion molecule 1) and vWF (von Willebrand factor) with immunocytochemistry staining. hCDSC also showed endothelial migration behaviourwhen cultured in fibrin-matrigel construct and were capable of forming vessels in vivo after implanting into nude mice. These data suggest that hCDSC could be the cells of choice in the cell-based therapy for pro-angiogenic purpose.

Original languageEnglish
Pages (from-to)1145-1153
Number of pages9
JournalCell Biology International
Volume36
Issue number12
DOIs
Publication statusPublished - 1 Dec 2012

Fingerprint

Chorion
Stem Cells
von Willebrand Factor
CD31 Antigens
Vascular Endothelial Growth Factor Receptor-2
Prostaglandins F
Cell- and Tissue-Based Therapy
Fibrin
Vascular Endothelial Growth Factor A
Immunohistochemistry
Messenger RNA
Nude Mice
Blood Vessels
Ischemia
Staining and Labeling
Gene Expression
Polymerase Chain Reaction
Growth

Keywords

  • Angiogenesis
  • Cell-based therapy
  • Fetal stem cells
  • Growth factors
  • Ischaemia
  • Placenta

ASJC Scopus subject areas

  • Cell Biology

Cite this

Endogenous and induced angiogenic characteristics of human chorion-derived stem cells. / Nur Fariha, Mohd Manzor; Kien Hui, Chua; Tan, Geok Chin; Lim, Yun Hsuen; Hayati, Abdul Rahman.

In: Cell Biology International, Vol. 36, No. 12, 01.12.2012, p. 1145-1153.

Research output: Contribution to journalArticle

Nur Fariha, Mohd Manzor ; Kien Hui, Chua ; Tan, Geok Chin ; Lim, Yun Hsuen ; Hayati, Abdul Rahman. / Endogenous and induced angiogenic characteristics of human chorion-derived stem cells. In: Cell Biology International. 2012 ; Vol. 36, No. 12. pp. 1145-1153.
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