Embryo apoptosis identification: Oocyte grade or cleavage stage?

Noraina Mohd Bakri, Siti Fatimah Ibrahim, Nurul Atikah Osman, Nurhaslina Hasan, Jaffar Farah Hanan Fathihah, Zulaiha Abdul Rahman, Khairul Osman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Apoptosis is a programed cell death that is vital for tissue homeostasis. However, embryo apoptosis had been known to be related to embryo fragmentation which should be avoided in in vitro fertilization (IVF). The purpose of this study was to evaluate the relationship of embryo apoptosis with the grade of immature oocytes and cleavage stage of in vitro produced (IVP) cattle embryos. This study consisted of 345 oocytes collected through ovary slicing. Immature oocytes were graded as A, B and C. This grading was based on cumulus cell thickness and compactness. All oocytes then underwent an in vitro maturation (IVM) procedure. An IVF was done 24. h after IVM culture. Prior to staining, stage of cleaved embryos was determined and classified as either 2, 4, 8 or >8-cell embryo stage. Apoptosis status of cleaved IVP embryos was determined by using annexin V-FITC staining technique at 48 and 72. h post insemination (hpi). Apoptosis status for each embryo was classified as either early or late. The result showed that there was no significant difference (p >. 0.05) of apoptosis status among grade A, B and C embryos. All grades of oocytes showed embryo apoptosis where 1.5% late apoptosis for grade A, 4.5% and 10.4% of early and late apoptosis for grade B and grade C. Early apoptosis was not seen in grade A embryo. We also noted no significant difference (p >. 0.05) of apoptosis status between 2, 4, 8 and >8-cell embryo stage. Early apoptosis was also not seen in >8-cell stage. Even though there were no differences in apoptosis expression between the three classes, the cleavage rate of grade A oocytes was significantly higher (p

Original languageEnglish
JournalSaudi Journal of Biological Sciences
DOIs
Publication statusAccepted/In press - 27 Jun 2015

Fingerprint

Oocytes
embryo (animal)
oocytes
Embryonic Structures
apoptosis
Apoptosis
in vitro fertilization
Fertilization in Vitro
immatures
cells
Staining and Labeling
Cumulus Cells
slicing
Fluorescein-5-isothiocyanate
Insemination
Annexin A5
insemination
cell death
Ovary
homeostasis

Keywords

  • Apoptosis
  • Cleavage stage
  • Oocyte grading

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Embryo apoptosis identification : Oocyte grade or cleavage stage? / Bakri, Noraina Mohd; Ibrahim, Siti Fatimah; Osman, Nurul Atikah; Hasan, Nurhaslina; Farah Hanan Fathihah, Jaffar; Rahman, Zulaiha Abdul; Osman, Khairul.

In: Saudi Journal of Biological Sciences, 27.06.2015.

Research output: Contribution to journalArticle

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title = "Embryo apoptosis identification: Oocyte grade or cleavage stage?",
abstract = "Apoptosis is a programed cell death that is vital for tissue homeostasis. However, embryo apoptosis had been known to be related to embryo fragmentation which should be avoided in in vitro fertilization (IVF). The purpose of this study was to evaluate the relationship of embryo apoptosis with the grade of immature oocytes and cleavage stage of in vitro produced (IVP) cattle embryos. This study consisted of 345 oocytes collected through ovary slicing. Immature oocytes were graded as A, B and C. This grading was based on cumulus cell thickness and compactness. All oocytes then underwent an in vitro maturation (IVM) procedure. An IVF was done 24. h after IVM culture. Prior to staining, stage of cleaved embryos was determined and classified as either 2, 4, 8 or >8-cell embryo stage. Apoptosis status of cleaved IVP embryos was determined by using annexin V-FITC staining technique at 48 and 72. h post insemination (hpi). Apoptosis status for each embryo was classified as either early or late. The result showed that there was no significant difference (p >. 0.05) of apoptosis status among grade A, B and C embryos. All grades of oocytes showed embryo apoptosis where 1.5{\%} late apoptosis for grade A, 4.5{\%} and 10.4{\%} of early and late apoptosis for grade B and grade C. Early apoptosis was not seen in grade A embryo. We also noted no significant difference (p >. 0.05) of apoptosis status between 2, 4, 8 and >8-cell embryo stage. Early apoptosis was also not seen in >8-cell stage. Even though there were no differences in apoptosis expression between the three classes, the cleavage rate of grade A oocytes was significantly higher (p",
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