Electrospun Biopolyesters as Drug Screening Platforms for Corneal Keratocytes

Pedram Azari, Ng Sook Luan, Seng Neon Gan, Rosiyah Yahya, Chiow San Wong, Chua Kien Hui, Belinda Pingguan-Murphy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In vitro drug screening techniques provide rapid and easy to analyze data, while saving a lot of animals from being sacrificed. An important part of any in vitro drug screening platform is a biomaterial which promotes cell growth and proliferation. The potential of electrospun scaffolds made of polyhydroxybutyrate (PHB), poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV), and polycaprolactone (PCL) were studied to serve as drug screening platform for corneal keratocyte tissues. The results showed that the proliferation rate was slightly higher for PCL and PHBV on day 7. Gene expression results showed that PCL was the best in maintaining keratocyte genes.

Original languageEnglish
Pages (from-to)785-791
Number of pages7
JournalInternational Journal of Polymeric Materials and Polymeric Biomaterials
Volume64
Issue number15
DOIs
Publication statusPublished - 22 Nov 2015

Fingerprint

Polycaprolactone
Screening
Pharmaceutical Preparations
Acids
Cell proliferation
Cell growth
Biocompatible Materials
Gene expression
Biomaterials
Scaffolds
Animals
Genes
Tissue
polycaprolactone

Keywords

  • Biopolyesters
  • corneal keratocytes
  • electrospinning
  • polycaprolactone
  • polyhydroxybutyrate
  • polyhydroxybutyrate-co-hydroxyvalerate

ASJC Scopus subject areas

  • Polymers and Plastics
  • Analytical Chemistry
  • Chemical Engineering(all)

Cite this

Electrospun Biopolyesters as Drug Screening Platforms for Corneal Keratocytes. / Azari, Pedram; Luan, Ng Sook; Gan, Seng Neon; Yahya, Rosiyah; Wong, Chiow San; Kien Hui, Chua; Pingguan-Murphy, Belinda.

In: International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 64, No. 15, 22.11.2015, p. 785-791.

Research output: Contribution to journalArticle

Azari, Pedram ; Luan, Ng Sook ; Gan, Seng Neon ; Yahya, Rosiyah ; Wong, Chiow San ; Kien Hui, Chua ; Pingguan-Murphy, Belinda. / Electrospun Biopolyesters as Drug Screening Platforms for Corneal Keratocytes. In: International Journal of Polymeric Materials and Polymeric Biomaterials. 2015 ; Vol. 64, No. 15. pp. 785-791.
@article{2ecaf11534144cab8a51b49bdb4d4b1c,
title = "Electrospun Biopolyesters as Drug Screening Platforms for Corneal Keratocytes",
abstract = "In vitro drug screening techniques provide rapid and easy to analyze data, while saving a lot of animals from being sacrificed. An important part of any in vitro drug screening platform is a biomaterial which promotes cell growth and proliferation. The potential of electrospun scaffolds made of polyhydroxybutyrate (PHB), poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV), and polycaprolactone (PCL) were studied to serve as drug screening platform for corneal keratocyte tissues. The results showed that the proliferation rate was slightly higher for PCL and PHBV on day 7. Gene expression results showed that PCL was the best in maintaining keratocyte genes.",
keywords = "Biopolyesters, corneal keratocytes, electrospinning, polycaprolactone, polyhydroxybutyrate, polyhydroxybutyrate-co-hydroxyvalerate",
author = "Pedram Azari and Luan, {Ng Sook} and Gan, {Seng Neon} and Rosiyah Yahya and Wong, {Chiow San} and {Kien Hui}, Chua and Belinda Pingguan-Murphy",
year = "2015",
month = "11",
day = "22",
doi = "10.1080/00914037.2015.1030648",
language = "English",
volume = "64",
pages = "785--791",
journal = "International Journal of Polymeric Materials",
issn = "0091-4037",
publisher = "Taylor and Francis Ltd.",
number = "15",

}

TY - JOUR

T1 - Electrospun Biopolyesters as Drug Screening Platforms for Corneal Keratocytes

AU - Azari, Pedram

AU - Luan, Ng Sook

AU - Gan, Seng Neon

AU - Yahya, Rosiyah

AU - Wong, Chiow San

AU - Kien Hui, Chua

AU - Pingguan-Murphy, Belinda

PY - 2015/11/22

Y1 - 2015/11/22

N2 - In vitro drug screening techniques provide rapid and easy to analyze data, while saving a lot of animals from being sacrificed. An important part of any in vitro drug screening platform is a biomaterial which promotes cell growth and proliferation. The potential of electrospun scaffolds made of polyhydroxybutyrate (PHB), poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV), and polycaprolactone (PCL) were studied to serve as drug screening platform for corneal keratocyte tissues. The results showed that the proliferation rate was slightly higher for PCL and PHBV on day 7. Gene expression results showed that PCL was the best in maintaining keratocyte genes.

AB - In vitro drug screening techniques provide rapid and easy to analyze data, while saving a lot of animals from being sacrificed. An important part of any in vitro drug screening platform is a biomaterial which promotes cell growth and proliferation. The potential of electrospun scaffolds made of polyhydroxybutyrate (PHB), poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV), and polycaprolactone (PCL) were studied to serve as drug screening platform for corneal keratocyte tissues. The results showed that the proliferation rate was slightly higher for PCL and PHBV on day 7. Gene expression results showed that PCL was the best in maintaining keratocyte genes.

KW - Biopolyesters

KW - corneal keratocytes

KW - electrospinning

KW - polycaprolactone

KW - polyhydroxybutyrate

KW - polyhydroxybutyrate-co-hydroxyvalerate

UR - http://www.scopus.com/inward/record.url?scp=84929887548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929887548&partnerID=8YFLogxK

U2 - 10.1080/00914037.2015.1030648

DO - 10.1080/00914037.2015.1030648

M3 - Article

AN - SCOPUS:84929887548

VL - 64

SP - 785

EP - 791

JO - International Journal of Polymeric Materials

JF - International Journal of Polymeric Materials

SN - 0091-4037

IS - 15

ER -