Electrochemical sunset yellow biosensor based on photocured polyacrylamide membrane for food dye monitoring

Normazida Rozi, Amalina Ahmad, Yook Heng Lee, Kee Shyuan Loh, Sharina Abu Hanifah

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An enzyme-based electrochemical biosensor was investigated for the analysis of Sunset Yellow synthetic food dye. A glassy carbon electrode was coated with a poly(acrylamide-co-ethyl methacrylate) membrane to immobilize laccase using a single-step photopolymerization procedure. Poly(acrylamide-co-ethyl methacrylate) membrane was demonstrated to have acceptable water absorption and suitable for biosensor application. Sunset Yellow biosensor exhibited a linear response range from 0.08 to 10.00 µM with a detection limit of 0.02 µM. This biosensor was successfully used to determine Sunset Yellow in soft drinks with recoveries of 99.0-101.6%. The method was validated using high-performance liquid chromatography, indicating the biosensor can be as a promising alternative method for Sunset Yellow detection.

Original languageEnglish
Article number101
JournalSensors (Switzerland)
Volume18
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

sunset
Biosensing Techniques
Polyacrylates
bioinstrumentation
food
Biosensors
Coloring Agents
Dyes
dyes
membranes
Membranes
Food
Monitoring
Methacrylates
Acrylamide
synthetic food
Synthetic foods
Carbonated Beverages
Laccase
Photopolymerization

Keywords

  • Electrochemical biosensor
  • Laccase
  • Photopolymerization
  • Sunset yellow
  • Synthetic food dye

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Electrochemical sunset yellow biosensor based on photocured polyacrylamide membrane for food dye monitoring. / Rozi, Normazida; Ahmad, Amalina; Lee, Yook Heng; Loh, Kee Shyuan; Abu Hanifah, Sharina.

In: Sensors (Switzerland), Vol. 18, No. 1, 101, 01.01.2018.

Research output: Contribution to journalArticle

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