Abstract
An enzyme-based electrochemical biosensor was investigated for the analysis of Sunset Yellow synthetic food dye. A glassy carbon electrode was coated with a poly(acrylamide-co-ethyl methacrylate) membrane to immobilize laccase using a single-step photopolymerization procedure. Poly(acrylamide-co-ethyl methacrylate) membrane was demonstrated to have acceptable water absorption and suitable for biosensor application. Sunset Yellow biosensor exhibited a linear response range from 0.08 to 10.00 µM with a detection limit of 0.02 µM. This biosensor was successfully used to determine Sunset Yellow in soft drinks with recoveries of 99.0-101.6%. The method was validated using high-performance liquid chromatography, indicating the biosensor can be as a promising alternative method for Sunset Yellow detection.
| Original language | English |
|---|---|
| Article number | 101 |
| Journal | Sensors (Switzerland) |
| Volume | 18 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2018 |
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Keywords
- Electrochemical biosensor
- Laccase
- Photopolymerization
- Sunset yellow
- Synthetic food dye
ASJC Scopus subject areas
- Analytical Chemistry
- Atomic and Molecular Physics, and Optics
- Biochemistry
- Instrumentation
- Electrical and Electronic Engineering
Cite this
Electrochemical sunset yellow biosensor based on photocured polyacrylamide membrane for food dye monitoring. / Rozi, Normazida; Ahmad, Amalina; Lee, Yook Heng; Loh, Kee Shyuan; Abu Hanifah, Sharina.
In: Sensors (Switzerland), Vol. 18, No. 1, 101, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electrochemical sunset yellow biosensor based on photocured polyacrylamide membrane for food dye monitoring
AU - Rozi, Normazida
AU - Ahmad, Amalina
AU - Lee, Yook Heng
AU - Loh, Kee Shyuan
AU - Abu Hanifah, Sharina
PY - 2018/1/1
Y1 - 2018/1/1
N2 - An enzyme-based electrochemical biosensor was investigated for the analysis of Sunset Yellow synthetic food dye. A glassy carbon electrode was coated with a poly(acrylamide-co-ethyl methacrylate) membrane to immobilize laccase using a single-step photopolymerization procedure. Poly(acrylamide-co-ethyl methacrylate) membrane was demonstrated to have acceptable water absorption and suitable for biosensor application. Sunset Yellow biosensor exhibited a linear response range from 0.08 to 10.00 µM with a detection limit of 0.02 µM. This biosensor was successfully used to determine Sunset Yellow in soft drinks with recoveries of 99.0-101.6%. The method was validated using high-performance liquid chromatography, indicating the biosensor can be as a promising alternative method for Sunset Yellow detection.
AB - An enzyme-based electrochemical biosensor was investigated for the analysis of Sunset Yellow synthetic food dye. A glassy carbon electrode was coated with a poly(acrylamide-co-ethyl methacrylate) membrane to immobilize laccase using a single-step photopolymerization procedure. Poly(acrylamide-co-ethyl methacrylate) membrane was demonstrated to have acceptable water absorption and suitable for biosensor application. Sunset Yellow biosensor exhibited a linear response range from 0.08 to 10.00 µM with a detection limit of 0.02 µM. This biosensor was successfully used to determine Sunset Yellow in soft drinks with recoveries of 99.0-101.6%. The method was validated using high-performance liquid chromatography, indicating the biosensor can be as a promising alternative method for Sunset Yellow detection.
KW - Electrochemical biosensor
KW - Laccase
KW - Photopolymerization
KW - Sunset yellow
KW - Synthetic food dye
UR - http://www.scopus.com/inward/record.url?scp=85039946419&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85039946419&partnerID=8YFLogxK
U2 - 10.3390/s18010101
DO - 10.3390/s18010101
M3 - Article
VL - 18
JO - Sensors (Switzerland)
JF - Sensors (Switzerland)
SN - 1424-8220
IS - 1
M1 - 101
ER -