Electrochemical DNA biosensor for potential carcinogen detection in food sample

Nor Diyana Md Sani, Yook Heng Lee, Radha Swathe Priya Malon Marugan, Nor Fadilah Rajab

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The presence of carcinogens in food is a major food safety concern. A nanocomposite-based electrochemical DNA biosensor was constructed for potential carcinogen detection in food samples by immobilizing amine terminated single stranded DNA onto silica nanospheres deposited onto a screen-printed electrode modified using gold nanoparticles. The effect of three different DNA sequences: 15-base guanine, 24-base guanine and 24-base adenine-thymine rich DNA on carcinogen (formaldehyde and acrylamide) detection was evaluated. The competitive binding of the DNA with the carcinogen and electroactive indicator, Methylene blue (MB) was measured using differential pulse voltammetry. Optimization studies were conducted for MB concentration and accumulation time, DNA concentration, buffer concentration, pH and ionic strength. Overall, the 24-base guanine rich DNA yielded the best results with a detection limit of 0.0001 ppm, linear range between 0.0001 ppm and 0.1 ppm and reproducibility below 5% R.S.D. Finally, the results obtained using the biosensor were validated using Ames test.

Original languageEnglish
Pages (from-to)503-510
Number of pages8
JournalFood Chemistry
Volume269
DOIs
Publication statusPublished - 15 Dec 2018

Fingerprint

biosensors
Biosensing Techniques
carcinogens
Biosensors
Carcinogens
foods
guanine
Guanine
Food
DNA
methylene blue
Methylene Blue
sampling
nanospheres
Nanospheres
nanocomposites
Ames test
Nanocomposites
Food safety
thymine

Keywords

  • 23-Aminopropyl-triethoxysilane (PubChem CID: 110154)
  • Acetic acid (PubChem CID: 176)
  • Acetyl acetone (PubChem CID: 31261)
  • Ammonia (PubChem CID: 222)
  • Ammonium Acetate (PubChem CID: 517165)
  • Carcinogens
  • Di-potassium hydrogen phosphate (PubChem CID: 24450)
  • Electrochemical biosensor
  • Ethanol (PubChem CID: 702)
  • Glutaraldehyde (PubChem CID: 3485)
  • Gold nanoparticles
  • Guanine rich DNA
  • Mercaptoethanol (PubChem CID: 1567)
  • Potassium chloride (PubChem CID: 4873)
  • Potassium dihydrogen phosphate (PubChem CID: 516951)
  • Potassium ferricyanide (PubChem CID: 26250)
  • Silica nanospheres
  • Sulfuric acid (PubChem CID: 1118)
  • Tetraethoxysilane (PubChem CID: 6517)
  • Toxicity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Electrochemical DNA biosensor for potential carcinogen detection in food sample. / Sani, Nor Diyana Md; Lee, Yook Heng; Marugan, Radha Swathe Priya Malon; Rajab, Nor Fadilah.

In: Food Chemistry, Vol. 269, 15.12.2018, p. 503-510.

Research output: Contribution to journalArticle

Sani, Nor Diyana Md ; Lee, Yook Heng ; Marugan, Radha Swathe Priya Malon ; Rajab, Nor Fadilah. / Electrochemical DNA biosensor for potential carcinogen detection in food sample. In: Food Chemistry. 2018 ; Vol. 269. pp. 503-510.
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AB - The presence of carcinogens in food is a major food safety concern. A nanocomposite-based electrochemical DNA biosensor was constructed for potential carcinogen detection in food samples by immobilizing amine terminated single stranded DNA onto silica nanospheres deposited onto a screen-printed electrode modified using gold nanoparticles. The effect of three different DNA sequences: 15-base guanine, 24-base guanine and 24-base adenine-thymine rich DNA on carcinogen (formaldehyde and acrylamide) detection was evaluated. The competitive binding of the DNA with the carcinogen and electroactive indicator, Methylene blue (MB) was measured using differential pulse voltammetry. Optimization studies were conducted for MB concentration and accumulation time, DNA concentration, buffer concentration, pH and ionic strength. Overall, the 24-base guanine rich DNA yielded the best results with a detection limit of 0.0001 ppm, linear range between 0.0001 ppm and 0.1 ppm and reproducibility below 5% R.S.D. Finally, the results obtained using the biosensor were validated using Ames test.

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