Electrochemical biosensor immobilization of formaldehyde dehydrogenase with Nafion for determination of formaldehyde from Indian mackerel (Rastrelliger kanagurta) fish

Nurindang Marzuki, Fatimah Abu Bakar, Abu Bakar Salleh, Yook Heng Lee, Nor Azah Yusof, Shafiquzzaman Siddiquee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An electrochemical biosensor was developed based on formaldehyde dehydrogenase immobilized with Nafion membrane for determination of formaldehyde in fish. The enzyme was immobilized through the entrapment technique and measured based on the reduction of β-nicotinamide adenine dinucleotide. The response time of the formaldehyde biosensor was <1 min, with an optimum pH of 8. The optimum enzyme loading and NAD + concentrations were found at 30 mg/mL and 0.5 mM, respectively. Using the formaldehyde biosensor, a linear response of formaldehyde showed a range of 0.1 to 10 ppm and a detection limit of 0.016 ppm. In application of Nash method, the samples were stored at 4°C ± 1 for 10 days. With the two combined methods, a linear correlation coefficient with R 2 = 0.9982 (y = 0.956x - 0.014) was found. The developed formaldehyde biosensor showed a good reproducibility, long storage stability (more than 6 months stored at 4°C), and also effective monitoring of formaldehyde level in Indian mackerel (Rastrelliger kanagurta) fish.

Original languageEnglish
Pages (from-to)534-542
Number of pages9
JournalCurrent Analytical Chemistry
Volume8
Issue number4
DOIs
Publication statusPublished - 2012

Fingerprint

glutathione-independent formaldehyde dehydrogenase
Biosensors
Fish
Formaldehyde
NAD
Enzymes
perfluorosulfonic acid
Membranes

Keywords

  • Electrochemical biosensor
  • Formaldehyde
  • Formaldehyde dehydrogenase
  • Indian mackerel
  • Nafion

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Electrochemical biosensor immobilization of formaldehyde dehydrogenase with Nafion for determination of formaldehyde from Indian mackerel (Rastrelliger kanagurta) fish. / Marzuki, Nurindang; Bakar, Fatimah Abu; Salleh, Abu Bakar; Lee, Yook Heng; Yusof, Nor Azah; Siddiquee, Shafiquzzaman.

In: Current Analytical Chemistry, Vol. 8, No. 4, 2012, p. 534-542.

Research output: Contribution to journalArticle

Marzuki, Nurindang ; Bakar, Fatimah Abu ; Salleh, Abu Bakar ; Lee, Yook Heng ; Yusof, Nor Azah ; Siddiquee, Shafiquzzaman. / Electrochemical biosensor immobilization of formaldehyde dehydrogenase with Nafion for determination of formaldehyde from Indian mackerel (Rastrelliger kanagurta) fish. In: Current Analytical Chemistry. 2012 ; Vol. 8, No. 4. pp. 534-542.
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