Electricity generation coupled to oxidation of propionate in a microbial fuel cell

Jae Kyung Jang, In Seop Chang, Hwa Yeon Hwang, Yeng Fung Choo, JiYoung Lee, Kyung Suk Cho, Byung Hong Kim, Kenneth H. Nealson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Propionate was used as fuel to enrich an electrochemically-active microbial consortium in a microbial fuel cell, and the bacterial consortium was analyzed by culture-independent methods including denaturing gradient gel electrophoresis (DGGE) of the 16S rDNA, and by fluorescent in situ hybridization (FISH). MFCs fed with propionate produced a current of 4.88 ± 0.1 mA stably on 100 mg propionate/l as COD within 3 weeks of the enrichment. When the MFCs were fed with H2-saturated fuel containing propionate, the current dropped to 3.82 ± 0.07 mA. The maximum current generated was up to 8.8 mA when MFCs were fed with 200 mg propionate/l as COD. The DGGE of 16S rDNA showed that propionate-enriched MFCs have a different bacterial population from that enriched with acetate and from the inoculum used for enrichment. The major member (42%) of the consortium was an unidentified bacterium followed by γ, β, and δ-proteobacteria.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalBiotechnology Letters
Volume32
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

Fingerprint

Bioelectric Energy Sources
Microbial fuel cells
Electricity
Propionates
Electrophoresis
Gels
Oxidation
Bacteria
Denaturing Gradient Gel Electrophoresis
Ribosomal DNA
Microbial Consortia
Proteobacteria
Fluorescence In Situ Hybridization
Acetates
Population

Keywords

  • 16S rDNA
  • Bacterial community
  • Electricity generation
  • Microbial fuel cell (MFC)
  • Propionate

ASJC Scopus subject areas

  • Biotechnology

Cite this

Jang, J. K., Chang, I. S., Hwang, H. Y., Choo, Y. F., Lee, J., Cho, K. S., ... Nealson, K. H. (2009). Electricity generation coupled to oxidation of propionate in a microbial fuel cell. Biotechnology Letters, 32(1), 79-85. https://doi.org/10.1007/s10529-009-0118-y

Electricity generation coupled to oxidation of propionate in a microbial fuel cell. / Jang, Jae Kyung; Chang, In Seop; Hwang, Hwa Yeon; Choo, Yeng Fung; Lee, JiYoung; Cho, Kyung Suk; Kim, Byung Hong; Nealson, Kenneth H.

In: Biotechnology Letters, Vol. 32, No. 1, 01.2009, p. 79-85.

Research output: Contribution to journalArticle

Jang, JK, Chang, IS, Hwang, HY, Choo, YF, Lee, J, Cho, KS, Kim, BH & Nealson, KH 2009, 'Electricity generation coupled to oxidation of propionate in a microbial fuel cell', Biotechnology Letters, vol. 32, no. 1, pp. 79-85. https://doi.org/10.1007/s10529-009-0118-y
Jang, Jae Kyung ; Chang, In Seop ; Hwang, Hwa Yeon ; Choo, Yeng Fung ; Lee, JiYoung ; Cho, Kyung Suk ; Kim, Byung Hong ; Nealson, Kenneth H. / Electricity generation coupled to oxidation of propionate in a microbial fuel cell. In: Biotechnology Letters. 2009 ; Vol. 32, No. 1. pp. 79-85.
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