Eimeria tenella glucose-6-phosphate isomerase: Molecular characterization and assessment as a target for anti-coccidial control

S. S. Loo, D. P. Blake, A. Mohd-Adnan, R. Mohamed, Kiew Lian Wan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Limitations with current chemotherapeutic and vaccinal control of coccidiosis caused by Eimeria species continue to prompt development of novel controls, including the identification of new drug targets. Glucose-6-phosphate isomerase (G6-PI) has been proposed as a valid drug target for many protozoa, although polymorphism revealed by electrophoretic enzyme mobility has raised doubts for Eimeria. In this study we identified and sequenced the Eimeria tenella G6-PI orthologue (EtG6-PI) from the reference Houghton strain and confirmed its position within the prevailing taxonomic hierarchy, branching with the Apicomplexa and Plantae, distinct from the Animalia including the host, Gallus gallus. Comparison of the deduced 1647 bp EtG6-PI coding sequence with the 9016 bp genomic locus revealed 15 exons, all of which obey the intron-AG-/exon/-GT-intron splicing rule. Comparison with the Weybridge and Wisconsin strains revealed the presence of 33 single nucleotide polymorphisms (SNPs) and 14 insertion/deletion sites. Three SNPs were exonic and all yielded non-synonymous substitutions. Preliminary structural predictions suggest little association between the coding SNPs and key G6-PI catalytic residues or residues thought to be involved in the coordination of the G6-PI's substrate phosphate group. Thus, the significant polymorphism from its host orthologue and minimal intra-specific polymorphism suggest G6-PI remains a valid anti-coccidial drug target.

Original languageEnglish
Pages (from-to)1169-1177
Number of pages9
JournalParasitology
Volume137
Issue number8
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Eimeria tenella
glucose-6-phosphate isomerase
Glucose-6-Phosphate Isomerase
single nucleotide polymorphism
Single Nucleotide Polymorphism
Eimeria
genetic polymorphism
Introns
exons
introns
Exons
Pharmaceutical Preparations
Apicomplexa
Coccidiosis
drugs
Animalia
Gallus gallus
new drugs
coccidiosis
Plantae

Keywords

  • coccidiosis
  • drug target
  • Eimeria tenella
  • G6-PI

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

Eimeria tenella glucose-6-phosphate isomerase : Molecular characterization and assessment as a target for anti-coccidial control. / Loo, S. S.; Blake, D. P.; Mohd-Adnan, A.; Mohamed, R.; Wan, Kiew Lian.

In: Parasitology, Vol. 137, No. 8, 07.2010, p. 1169-1177.

Research output: Contribution to journalArticle

Loo, S. S. ; Blake, D. P. ; Mohd-Adnan, A. ; Mohamed, R. ; Wan, Kiew Lian. / Eimeria tenella glucose-6-phosphate isomerase : Molecular characterization and assessment as a target for anti-coccidial control. In: Parasitology. 2010 ; Vol. 137, No. 8. pp. 1169-1177.
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